ACAT2 (NM_005891) Human Untagged Clone

CAT#: SC320257

ACAT2 (untagged)-Human acetyl-CoA acetyltransferase 2 (ACAT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACAT2
• Synonyms: acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 2; cytosolic acetoacetyl-CoA thiolase; OTTHUMP00000017527
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005891.1 GGAGAAGCAAGATGAATGCAGGCTCAGATCCTGTGGTCATCGTCTCGGCGGCGCGGACCA
  TCATAGGTTCCTTCAATGGTGCCTTAGCTGCTGTTCCTGTCCAGGACCTGGGCTCCACTG
  TCATCAAAGAAGTCTTGAAGAGGGCCACTGTGGCTCCGGAAGATGTGTCTGAGGTCATCT
  TTGGACATGTCTTGGCAGCAGGCTGTGGGCAGAATCCTGTTAGACAAGCCAGTGTGGGTG
  CAGGAATTCCCTACTCTGTTCCAGCATGGAGCTGCCAGATGATCTGTGGGTCAGGCCTAA
  AAGCTGTGTGCCTTGCAGTCCAGTCAATAGGGATAGGAGACTCCAGCATTGTGGTTGCAG
  GAGGCATGGAAAATATGAGCAAGGCTCCTCACTTGGCTTACTTGAGAACAGGAGTAAAGA
  TAGGTGAGATGCCACTGACTGACAGTATACTCTGTGATGGTCTTACAGATGCATTTCACA
  ACTGTCATATGGGTATTACAGCTGAAAATGTAGCCAAAAAATGGCAAGTGAGTAGAGAAG
  ATCAGGACAAGGTTGCAGTTCTGTCCCAGAACAGGACAGAGAATGCACAGAAAGCTGGCC
  ATTTTGACAAAGAGATTGTACCAGTTTTGGTGTCAACTAGAAGAGGTCTTATTGAAGTTA
  AAACAGATGAGTTTCCTCGCCATGGGAGCAACATAGAAGCCATGTCCAAGCTAAAGCCTT
  ACTTTCTTACTGATGGAACGGGAACAGTCACCCCAGCCAATGCTTCAGGAATAAATGATG
  GTGCTGCAGCTGTCGTTCTTATGAAGAAGTCAGAAGCTGATAAACGTGGACTTACACCTT
  TAGCACGGATAGTTTCCTGGTCCCAAGTGGGTGTGGAGCCTTCCATTATGGGAATAGGAC
  CAATTCCAGCCATAAAGCAAGCTGTTACAAAAGCAGGTTGGTCACTGGAAGATGTTGACA
  TATTTGAAATCAATGAAGCCTTTGCAGCTGTCTCTGCTGCAATAGTTAAAGAACTTGGAT
  TAAACCCAGAGAAGGTCAATATTGAAGGAGGGGCTATAGCCTTGGGCCACCCTCTTGGAG
  CATCTGGCTGTCGAATTCTTGTGACCCTGTTACACACACTGGAGAGAATGGGCAGAAGTC
  GTGGTGTTGCAGCCCTGTGCATTGGGGGTGGGATGGGAATAGCAATGTGTGTTCAGAGAG
  AATGAATTGCTTAAACTTTGAACAACCTCAATTTCTTTTTAAACTAATAAAGTACTAGGT
  TGCAATATGTGAAATCAGAGGACCAAAGTACAGATGGAAACCATTTCCTACATCACAAAA
  ACCCAAGTTTACAGCTTGTACTTTACTTTAATGTGTAATACTCAACTCAAGGTACAAGAC
  AATTGCATTTAACATTGTTATAAATAAAAGGAACATCAGATCAATCAAAAAAAAAAAAAA
  AAA
• Restriction Sites: Please inquire
• ACCN: NM_005891
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005891.1, NP_005882.1
• RefSeq Size: 1490 bp
• RefSeq ORF: 1194 bp
• Locus ID: 39
• Cytogenetics: 6q25.3
• Domains: thiolase
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Fatty acid metabolism, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Tryptophan metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: 'The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the shorter isoform (1).