MSH5 (NM_002441) Human Untagged Clone

CAT#: SC320262

MSH5 (untagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSH5
• Synonyms: G7; MUTSH5; NG23; POF13
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002441.3 GCGGTCGGTCAGCGGGGCGTTCTCCCACCTGTAGCGACTCAGAGCCTCCAAGCTCATGGC
  CTCCTTAGGAGCGAACCCAAGGAGGACACCGCAGGGACCGAGACCTGGGGCGGCCTCCTC
  CGGCTTCCCCAGCCCGGCCCCAGTGCCGGGCCCCAGGGAGGCCGAGGAGGAGGAAGTCGA
  GGAGGAGGAGGAGCTGGCCGAGATCCATCTGTGTGTGCTGTGGAATTCAGGATACTTGGG
  CATTGCCTACTATGATACTAGTGACTCCACTATCCACTTCATGCCAGATGCCCCAGACCA
  CGAGAGCCTCAAGCTTCTCCAGAGAGTTCTGGATGAGATCAATCCCCAGTCTGTTGTTAC
  GAGTGCCAAACAGGATGAGAATATGACTCGATTTCTGGGAAAGCTTGCCTCCCAGGAGCA
  CAGAGAGCCTAAAAGACCTGAAATCATATTTTTGCCAAGTGTGGATTTTGGTCTGGAGAT
  AAGCAAACAACGCCTCCTTTCTGGAAACTACTCCTTCATCCCAGACGCCATGACTGCCAC
  TGAGAAAATCCTCTTCCTCTCTTCCATTATTCCCTTTGACTGCCTCCTCACAGTTCGAGC
  ACTTGGAGGGCTGCTGAAGTTCCTGGGTCGAAGAAGAATCGGGGTTGAACTGGAAGACTA
  TAATGTCAGCGTCCCCATCCTGGGCTTTAAGAAATTTATGTTGACTCATCTGGTGAACAT
  AGATCAAGACACTTACAGTGTTCTACAGATTTTTAAGAGTGAGTCTCACCCCTCAGTGTA
  CAAAGTGGCCAGTGGACTGAAGGAGGGGCTCAGCCTCTTTGGAATCCTCAACAGATGCCA
  CTGTAAGTGGGGAGAGAAGCTGCTCAGGCTATGGTTCACACGTCCGACTCATGACCTGGG
  GGAGCTCAGTTCTCGTCTGGACGTCATTCAGTTTTTTCTGCTGCCCCAGAATCTGGACAT
  GGCTCAGATGCTGCATCGGCTCCTGGGTCACATCAAGAACGTGCCTCTGATTCTGAAACG
  CATGAAGTTGTCCCACACCAAGGTCAGCGACTGGCAGGTTCTCTACAAGACTGTGTACAG
  TGCCCTGGGCCTGAGGGATGCCTGCCGCTCCCTGCCGCAGTCCATCCAGCTCTTTCGGGA
  CATTGCCCAAGAGTTCTCTGATGACCTGCACCATATCGCCAGCCTCATTGGGAAAGTAGT
  GGACTTTGAGGGCAGCCTTGCTGAAAATCGCTTCACAGTCCTCCCCAACATAGATCCTGA
  AATTGATGAGAAAAAGCGAAGACTGATGGGACTTCCCAGTTTCCTTACTGAGGTTGCCCG
  CAAGGAGCTGGAGAATCTGGACTCCCGTATTCCTTCATGCAGTGTCATCTACATCCCTCT
  GATTGGCTTCCTTCTTTCTATTCCCCGCCTGCCTTCCATGGTAGAGGCCAGTGACTTTGA
  GATTAATGGACTGGACTTCATGTTTCTCTCAGAGGAGAAGCTGCACTATCGTAGTGCCCG
  AACCAAGGAGCTGGATGCATTGCTGGGGGACCTGCACTGCGAGATCCGGGACCAGGAGAC
  GCTGCTGATGTACCAGCTACAGTGCCAGGTGCTGGCACGAGCAGCTGTCTTAACCCGAGT
  ATTGGACCTTGCCTCCCGCCTGGACGTCCTGCTGGCTCTTGCCAGTGCTGCCCGGGACTA
  TGGCTACTCAAGGCCGCGTTACTCCCCACAAGTCCTTGGGGTACGAATCCAGAATGGCAG
  ACATCCTCTGATGGAACTCTGTGCCCGAACCTTTGTGCCCAACTCCACAGAATGTGGTGG
  GGACAAAGGGAGGGTCAAAGTCATCACTGGACCCAACTCATCAGGGAAGAGCATATACCT
  CAAACAGGTAGGCTTGATCACATTCATGGCCCTGGTAGGCAGCTTTGTGCCAGCAGAGGA
  GGCCGAAATTGGGGCAGTAGACGCCATCTTCACACGAATTCATAGCTGCGAATCCATCTC
  CCTTGGCCTCTCCACCTTCATGATCGACCTCAACCAGCAGGTGGCGAAAGCAGTGAACAA
  TGCCACTGCACAGTCGCTGGTCCTTATTGATGAATTTGGAAAGGGAACCAACACGGTGGA
  TGGGCTCGCGCTTCTGGCCGCTGTGCTCCGACACTGGCTGGCACGTGGACCCACATGCCC
  CCACATCTTTGTGGCCACCAACTTTCTGAGCCTTGTTCAGCTACAACTGCTGCCACAAGG
  GCCCCTGGTGCAGTATTTGACCATGGAGACCTGTGAGGATGGCAACGATCTTGTCTTCTT
  CTATCAGGTTTGCGAAGGTGTTGCGAAGGCCAGCCATGCCTCCCACACAGCTGCCCAGGC
  TGGGCTTCCTGACAAGCTTGTGGCTCGTGGCAAGGAGGTCTCAGACTTGATCCGCAGTGG
  AAAACCCATCAAGCCTGTCAAGGATTTGCTAAAGAAGAACCAAATGGAAAATTGCCAGAC
  ATTAGTGGATAAGTTTATGAAACTGGATTTGGAAGATCCTAACCTGGACTTGAACGTTTT
  CATGAGCCAGGAAGTGCTGCCTGCTGCCACCAGCATCCTCTGAGAGTCCTTCCAGTGTCC
  TCCCCAGCCTCCTGAGACTCCGGTGGGCTGCCATGCCCTCTTTGTTTCCTTATCTCCCTC
  AGACGCAGAGTTTTTAGTTTCTCTAGAAATTTTGTTTCATATTAGGAATAAAGTTTATTT
  TGAAGAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002441
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002441.3, NP_002432.1
• RefSeq Size: 2903 bp
• RefSeq ORF: 2505 bp
• Locus ID: 4439
• Cytogenetics: 6p21.33
• Domains: MutS_V, MutS_III, MutS_IV
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases, including IgA deficiency, common variable immunodeficiency, and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (3) differs in the 5' UTR and uses alternate in-frame splice sites in both the central and 3' coding regions, compared to variant 1, resulting in an isoform (c) that is longer than isoform a. Both variants 3 and 4 encode the same isoform.