NDUFS2 (NM_004550) Human Untagged Clone

CAT#: SC320283

NDUFS2 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) (NDUFS2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFS2
• Synonyms: CI-49; MC1DN6
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004550.3 GCCCCAGGAGAGGCAGAGAGTGAGGGAAAGGGCCTGGCCGGCATGCACAGATAGGATCAC
  GGTCCTGGGAGAATTCCTGCTCTTATAGTCTAACCTACCATGGCTTCTCTTTTCTCAAGG
  CTCCCTCATGCTGCCCTTTGGCCCTAGTGGCTGGTTTCCAGGGCTGAGGGGACTGAGTGA
  GCTGCCTGAGAAAAGAGGGTAGGGAACAGAAAAGCCAGCCAGGAGCTGTGGGAGGAAACG
  CCCTCAGTAAAGATGACCGCGGTCACTGTTATCTAAACGCAAGTGAAGCCGAGTCACAGG
  ACCCGGATGTTGTCAGTTCGACGGTAAACGACCCTGCCAGCTTCCAAGAGGGCGGCTTCA
  CTGTGCGAATAGGTGAGAAGCCAAGAAGGAGGCGCGCTGGAGTTACTTCCGCCCGGTTCT
  CCTTCCCGCAGTCTGCAGCCGGAGTAAGATGGCGGCGCTGAGGGCTTTGTGCGGCTTCCG
  GGGCGTCGCGGCCCAGGTGCTGCGGCCTGGGGCTGGAGTCCGATTGCCGATTCAGCCCAG
  CAGAGGTGTTCGGCAGTGGCAGCCAGATGTGGAATGGGCACAGCAGTTTGGGGGAGCTGT
  TATGTACCCAAGCAAAGAAACAGCCCACTGGAAGCCTCCACCTTGGAATGATGTGGACCC
  TCCAAAGGACACAATTGTGAAGAACATTACCCTGAACTTTGGGCCCCAACACCCAGCAGC
  GCATGGTGTCCTGCGACTAGTGATGGAATTGAGTGGGGAGATGGTGCGGAAGTGTGATCC
  TCACATCGGGCTCCTGCACCGAGGCACTGAGAAGCTCATTGAATACAAGACCTATCTTCA
  GGCCCTTCCATACTTTGACCGGCTAGACTATGTGTCCATGATGTGTAACGAACAGGCCTA
  TTCTCTAGCTGTGGAGAAGTTGCTAAACATCCGGCCTCCTCCTCGGGCACAGTGGATCCG
  AGTGCTGTTTGGAGAAATCACACGTTTGTTGAACCACATCATGGCTGTGACCACACATGC
  CCTGGACCTTGGGGCCATGACCCCTTTCTTCTGGCTGTTTGAAGAAAGGGAGAAGATGTT
  TGAGTTCTACGAGCGAGTGTCTGGAGCCCGAATGCATGCTGCTTATATCCGGCCAGGAGG
  AGTGCACCAGGACCTACCCCTTGGGCTTATGGATGACATTTATCAGTTTTCTAAGAACTT
  CTCTCTTCGGCTTGATGAGTTGGAGGAGTTGCTGACCAACAATAGGATCTGGCGAAATCG
  GACAATTGACATTGGGGTTGTAACAGCAGAAGAAGCACTTAACTATGGTTTTAGTGGAGT
  GATGCTTCGGGGCTCAGGCATCCAGTGGGACCTGCGGAAGACCCAGCCCTATGATGTTTA
  CGACCAGGTTGAGTTTGATGTTCCTGTTGGTTCTCGAGGGGACTGCTATGATAGGTACCT
  GTGCCGGGTGGAGGAGATGCGCCAGTCCCTGAGAATTATCGCACAGTGTCTAAACAAGAT
  GCCTCCTGGGGAGATCAAGGTTGATGATGCCAAAGTGTCTCCACCTAAGCGAGCAGAGAT
  GAAGACTTCCATGGAGTCACTGATTCATCACTTTAAGTTGTATACTGAGGGCTACCAAGT
  TCCTCCAGGAGCCACATATACTGCCATTGAGGCTCCCAAGGGAGAGTTTGGGGTGTACCT
  GGTGTCTGATGGCAGCAGCCGCCCTTATCGATGCAAGATCAAGGCTCCTGGTTTTGCCCA
  TCTGGCTGGTTTGGACAAGATGTCTAAGGGACACATGTTGGCAGATGTCGTTGCCATCAT
  AGGTACCCAAGATATTGTATTTGGAGAAGTAGATCGGTGAGCAGGGGAGCAGCGTTTGAT
  CCCCCCTGCCTATCAGCTTCTTCTGTGGAGCCTGTTCCTCACTGGAAATTGGCCTCTGTG
  TGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTTCGTGTACACTTGGCTGTCAGGCTTTC
  TGTGCATGTACTAAAAAAGGAGAAATTATAATAAATTAGCCGTCTTGCGGCCCCTAGGCC
  TAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004550
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004550.3, NP_004541.1
• RefSeq Size: 2061 bp
• RefSeq ORF: 1392 bp
• Locus ID: 4720
• Cytogenetics: 1q23.3
• Domains: complex1_49Kd
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).