SLC12A4 (NM_005072) Human Untagged Clone

CAT#: SC320286

SLC12A4 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC12A4
• Synonyms: CTC-479C5.17; hKCC1; KCC1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005072.3 GGCGGGGACAGCGGCGGGGACAGCGGCGGGCGGCTGGGACGGCGGGTGCGGCGGGGCCGA
  GCCCGCACGATGCCTCACTTCACCGTGGTGCCAGTGGACGGGCCGAGGCGCGGCGACTAT
  GACAACCTCGAGGGGCTCAGTTGGGTGGACTACGGGGAGCGCGCCGAGCTGGATGACTCG
  GACGGACATGGCAACCACAGAGAGAGCAGCCCTTTTCTTTCCCCCTTGGAGGCTTCCAGA
  GGAATTGACTACTATGACAGGAACCTGGCACTGTTTGAGGAAGAGCTGGACATCCGCCCA
  AAGGTATCGTCTCTTCTGGGAAAGCTCGTCAGCTACACCAACCTCACCCAGGGCGCCAAA
  GAGCATGAGGAGGCCGAGAGTGGGGAGGGCACCCGCCGGAGGGCAGCCGAGGCACCCAGC
  ATGGGCACCCTCATGGGGGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTTATCCTC
  TTCCTGCGGCTGACCTGGATGGTGGGCACAGCAGGTGTGCTACAGGCCCTCCTCATCGTG
  CTTATCTGCTGCTGTTGTACCCTGCTGACGGCCATCTCCATGAGTGCCATCGCCACCAAC
  GGTGTGGTTCCAGCTGGGGGCTCCTATTTCATGATCTCTCGTTCACTGGGGCCAGAATTT
  GGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGAACAACATTCGCAGCAGCCATGTACATC
  CTGGGGGCCATCGAGATCTTGCTGACCTACATTGCCCCACCAGCTGCCATTTTTTACCCA
  TCGGGTGCTCATGACACGTCGAATGCCACTTTGAACAATATGCGTGTGTATGGGACCATT
  TTCCTGACCTTCATGACCCTGGTGGTGTTTGTGGGGGTCAAGTATGTGAACAAATTTGCC
  TCGCTCTTCCTGGCCTGTGTGATCATCTCCATCCTCTCCATCTATGCTGGGGGCATAAAG
  TCTATATTTGACCCTCCCGTGTTTCCGGTATGCATGCTGGGCAACAGGACCCTGTCCCGG
  GACCAGTTTGACATCTGTGCCAAGACAGCTGTAGTGGACAATGAGACAGTGGCCACCCAG
  CTATGGAGTTTCTTCTGCCACAGCCCCAACCTTACGACCGACTCCTGTGACCCCTACTTC
  ATGCTCAACAATGTGACCGAGATCCCTGGCATCCCCGGGGCAGCTGCTGGTGTGCTCCAG
  GAAAACCTGTGGAGCGCCTACCTGGAGAAGGGTGACATCGTGGAGAAGCATGGGCTGCCC
  TCCGCAGATGCCCCGAGCCTGAAGGAGAGCCTGCCTCTGTACGTGGTCGCTGACATCGCC
  ACATCCTTCACCGTGCTGGTCGGCATCTTCTTCCCTTCTGTAACAGGCATCATGGCTGGC
  TCAAACCGCTCTGGGGACCTTCGTGACGCCCAGAAGTCTATCCCTGTGGGGACCATTCTG
  GCCATCATTACAACTTCCCTCGTGTACTTCAGCAGTGTGGTTCTCTTTGGTGCCTGCATT
  GAGGGTGTGGTTCTCCGGGACAAGTATGGCGATGGTGTCAGCAGGAACTTGGTGGTGGGC
  ACACTGGCCTGGCCTTCACCCTGGGTCATCGTCATCGGCTCCTTCTTTTCAACGTGTGGC
  GCTGGCCTCCAGAGCCTCACAGGGGCACCACGCCTATTGCAGGCCATTGCCAAGGACAAC
  ATCATCCCCTTCCTCCGGGTGTTTGGCCACGGGAAGGTGAATGGTGAACCCACATGGGCA
  CTCCTCCTGACGGCACTCATCGCCGAGCTGGGCATCCTCATCGCCTCCCTCGACATGGTG
  GCCCCCATCTTATCCATGTTCTTTCTGATGTGCTACCTGTTCGTGAACCTCGCCTGTGCG
  GTGCAGACACTCCTGAGGACCCCCAACTGGCGGCCCCGGTTCAAGTACTATCACTGGGCG
  CTGTCCTTCCTGGGCATGAGTCTCTGCCTGGCCCTTATGTTTGTCTCCTCCTGGTACTAT
  GCCCTGGTGGCCATGCTCATCGCCGGCATGATCTACAAATACATCGAGTACCAAGGGGCT
  GAGAAGGAGTGGGGTGACGGGATCCGAGGCCTGTCCCTGAGCGCTGCCCGCTACGCGCTG
  TTGCGGCTGGAGGAGGGGCCTCCTCACACCAAGAACTGGCGGCCGCAGCTGCTGGTGCTG
  CTGAAGCTGGACGAGGACCTCCACGTGAAGTACCCGCGGCTCCTCACCTTCGCCTCCCAG
  CTCAAGGCTGGCAAGGGCCTGACCATTGTTGGTTCTGTCATCCAGGGGAGCTTCTTGGAG
  AGCTATGGCGAGGCTCAGGCCGCCGAGCAGACCATCAAGAACATGATGGAAATTGAGAAG
  GTGAAGGGCTTCTGCCAGGTGGTGGTGGCCAGCAAGGTGCGGGAGGGGCTGGCCCACCTC
  ATCCAGTCCTGTGGCCTGGGAGGCATGCGGCATAACTCCGTGGTGCTGGGCTGGCCCTAC
  GGCTGGCGACAGAGCGAGGACCCCCGTGCCTGGAAGACCTTCATTGACACCGTGCGCTGC
  ACTACGGCTGCCCACCTGGCCCTGCTCGTGCCCAAGAACATCGCCTTCTACCCCAGCAAC
  CACGAGCGCTACCTGGAGGGCCACATAGACGTGTGGTGGATCGTGCACGATGGTGGCATG
  CTCATGCTTCTGCCCTTCCTGCTGCGCCAGCATAAGGTCTGGAGGAAGTGCCGGATGCGC
  ATCTTCACAGTGGCCCAGATGGATGACAACAGCATCCAGATGAAGAAGGACCTGGCTGTC
  TTTCTGTACCATCTGCGCCTTGAGGCCGAGGTGGAGGTGGTGGAGATGCATAACAGTGAC
  ATCTCTGCATACACCTACGAGCGGACGCTGATGATGGAGCAGCGGTCGCAGATGCTGCGG
  CAGATGAGACTGACCAAGACTGAGCGGGAGCGAGAAGCCCAGCTGGTCAAGGATCGGCAC
  TCGGCCCTGAGGCTGGAGAGCCTGTACTCGGACGAGGAAGATGAGTCTGCAGTGGGGGCT
  GACAAGATCCAGATGACGTGGACCAGGGACAAGTACATGACTGAGACCTGGGACCCCAGC
  CATGCCCCTGACAATTTCCGGGAGCTGGTGCACATTAAGCCGGACCAATCCAATGTGCGG
  CGCATGCACACTGCTGTGAAGCTCAATGAAGTCATTGTCACGCGCTCCCACGACGCCCGC
  CTGGTTCTCCTAAACATGCCTGGCCCACCCAGGAACAGTGAGGGCGACGAGAACTACATG
  GAGTTCCTCGAGGTGCTGACCGAGGGCCTTGAGCGGGTGCTGTTGGTGCGCGGTGGTGGC
  CGTGAAGTCATCACCATCTACTCCTGAGCCCAGTGTCATCTTGTGGCCTGGAGTCGAGGT
  CTTGGCCAGGACATAACAAGCTGTGGTCTGGGGTAACAGCCTCTTCCCAGCACCCACCTG
  CCAGCCCTGCTTGCCTGGCCCTGTCCTGGACCCAGCTTTGCTAGGTCTCCTTGGAAACCA
  GGCCTGGGCCTCAAAATGGAGATGGATCCCAGGTCTTGTGGGACCCTGGGATGTTTGGGG
  ACTTTACTATCTAGCACCCCAGTAGGCCTGTCCTGGCCAGAGAAGACTGGTAGGGGCCGA
  GTGGGGTTTGAAGGCAGCCGGCCCGGCCCAGCCCAGGAGCGCTATTTATTGCATATTTAT
  TGTTTGGATGTCACCATCAGAGACGAAGGGAAGGGTAGCCAGGGAGGGAGTCCAGCCCAG
  CTGCCTGCAGGAAGATCTGGCTCAGTCTACTATGGGCAGGGCCCCCCACCAAGCTGAGCC
  GAATGGAGACAGCTGAGCTGAGGCCTGACTTTTTCAATAAAACATTGTGTAGTTCTGGGC
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005072
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005072.3, NP_005063.1
• RefSeq Size: 3884 bp
• RefSeq ORF: 3258 bp
• Locus ID: 6560
• Cytogenetics: 16q22.1
• Domains: KCl_Cotrans_1
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a.