PAK4 (NM_001014831) Human Untagged Clone

CAT#: SC320565

PAK4 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAK4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001014831.2 GCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAGGGAGGCGCGAGGGCGCGGAG
  TTCCAGGTCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAGCCGATGAGTAACCCGAA
  GCCCCTAGAGGAGTGGTCACCTGCCTGAGGGCACTTCTGTCCCACCAGCATCAGACCAGG
  TGTGTGCAGGCTCTCTATGGGATGCCGCCATCAGAATGTCTCTTCCTCAGCCCCTTTGTT
  TTTTAAAGTGGCTTTGGTCGACCATTTGCTCTGTGCTTGGTACTGTGTCAAGGGCCCGAT
  TGAAGACCACAGATCCTAAAGATAGGGGAGTGTCGCGACAAGGCTGCCTGGGGTGCCCTC
  TTAACCCAGGGGATCAGTAACCCTGTGCTGAGTCAATATCCAAAGGCCGCACCGAGTCCC
  CGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGA
  GCACCGCGTGCACACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCA
  GTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGACCCCGCCTG
  CATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAA
  AGATGGGGCCCTCACGCTGCTGCTGGACGAGTTTGAGAACATGTCGGTGACACGCTCCAA
  CTCCCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCC
  AGAGGAGCCGGCCACCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCAGCCGAGGCCGGTT
  CGCCGGTCACAGCGAGGCGGGTGGCGGCAGTGGTGACAGGCGACGGGCGGGGCCAGAGAA
  GAGGCCCAAGTCTTCCAGGGAGGGCTCAGGGGGTCCCCAGGAGTCCTCCCGGGACAAACG
  CCCCCTCTCCGGGCCTGATGTCGGCACCCCCCAGCCTGCTGGTCTGGCCAGTGGGGCGAA
  ACTGGCAGCTGGCCGGCCCTTTAACACCTACCCGAGGGCTGACACGGACCACCCATCCCG
  GGGTGCCCAGGGGGAGCCTCATGACGTGGCCCCTAACGGGCCATCAGCGGGGGGCCTGGC
  CATCCCCCAGTCCTCCTCCTCCTCCTCCCGGCCTCCCACCCGAGCCCGAGGTGCCCCCAG
  CCCTGGAGTGCTGGGACCCCACGCCTCAGAGCCCCAGCTGGCCCCTCCAGCCTGCACCCC
  CGCCGCCCCTGCTGTTCCTGGGCCCCCTGGCCCCCGCTCACCACAGCGGGAGCCACAGCG
  AGTATCCCATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGCGACCCCCG
  CTCCTACCTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCATCGC
  CACCGTGCGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACCTGCGCAAGCAGCA
  GAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACCAGCACGAGAATGT
  GGTGGAGATGTACAACAGCTACCTGGTGGGGGACGAGCTCTGGGTGGTCATGGAGTTCCT
  GGAAGGAGGCGCCCTCACCGACATCGTCACCCACACCAGGATGAACGAGGAGCAGATCGC
  GGCCGTGTGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCATCCA
  CCGGGACATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCAGGGTGAAGCTGTCAGA
  CTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGTCGCTGGTCGGCAC
  GCCCTACTGGATGGCCCCAGAGCTCATCTCCCGCCTTCCCTACGGGCCAGAGGTAGACAT
  CTGGTCGCTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGCCCCCCTACTTCAACGA
  GCCACCCCTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAACCT
  GCACAAGGTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGGTGCGAGACCCTGC
  CCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCAAGGCAGGGCCGCC
  TGCCAGCATCGTGCCCCTCATGCGCCAGAACCGCACCAGATGAGGCCCAGCGCCCTTCCC
  CTCAACCAAAGAGCCCCCCGGGTCACCCCCGCCCCACTGAGGCCAGTAGGGGGCCAGGCC
  TCCCACTCCTCCCAGCCCGGGAGATGCTCCGCGTGGCACCACCCTCCTTGCTGGGGGTAG
  ATGAGACCCTACTACTGAACTCCAGTTTTGATCTCGTGACTTTTAGAAAAACACAGGGAC
  TCGTGGGAGCAAGCGAGGCTCCCAGGACCCCCACCCTCTGGGACAGGCCCTCCCCCATGT
  TCTTCTGTCTCCAGGAAGGGCAGCGGCCCTCCCATCACTGGAAGTCTGCAGTGGGGGTCG
  CTGGGGGTGGAGAGAACACTAAGAGGTGAACATGTATGAGTGTGTGCACGCGTGTGAGTG
  TGCATGTGTGTGTGTGCAAAGGTCCAGCCACCCCGTCCTCCAGCCTGCAAGGGGTGTCTG
  GCGCCTTGCCTGACACCCAGCCCCCTCTCCCCCTGAGCCATTGTGGGGGTCGATCATGAA
  TGTCCGAAGAGTGGCCTTTTCCCGTAGCCCTGCGCCCCCTTTCTGTGGCTGGATGGGGAG
  ACAGGTCAGGGCCCCCCACCCTCTCCAGCCCCTGCAGCAAATGACTACTGCACCTGGACA
  GCCTCCTCTTTTCTAGAAGTCTATTTATATTGTCATTTTATAACACTCTAGCCCCTGCCC
  TTATTGGGGGACAGATGGTCCCTGTCCTGCGGGGTGGCCCTGGCAGAACCACTGCCTGAA
  GAACCAGGTTCCTGCCCGGTCAGCGCAGCCCCAGCCCGCCCACCCCTGCCTCGAGTTAGT
  TTTACAATTAAAACATTGTCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001014831
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014831.2, NP_001014831.1
• RefSeq Size: 3064 bp
• RefSeq ORF: 1776 bp
• Locus ID: 10298
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has an additional segment in the 5' UTR, and encodes the same isoform (1), as compared to variant 1.