MRPL4 (NM_146388) Human Untagged Clone

CAT#: SC320600

MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL4
• Synonyms: CGI-28; L4mt
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_146388.1 GTCGCGTTCCCCAGTGTTACGGAGGGTCCTTGAGGCAGGAGTGAAAATTGGGTCTGGGGG
  TTAGTCCTGGGGTGGAGGTCTGGGCACGCCGGGTCGGACCCCCTCCATCTTCGGTTTTGC
  ACACCCCGCTTTCCAGCGCGGAGTCGCGGCGGGTAGGGCGGCGTCGCGTGCGTGACGTCA
  TCCAGCGGCGCCTCGCGAGGCTCCAGTGGCCTTGACCTCCCGCGGCGTGGGAGGCTGCGC
  GGCGATGCTGCAGTTCGTCCGGGCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCA
  GGGCCTGAGTTCCCTGGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGC
  GAGCGAGGGTCTCCCGGAGCCCGTGCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCG
  ACGCCCAGTGCAGGCCTGGGTCGAGTCCTTGCGGGGCTTCGAGCAGGAGCGCGTGGGCCT
  GGCCGACCTGCACCCCGATGTTTTCGCCACCGCGCCCAGGCTGGACATACTGCACCAGGT
  TGCTATGTGGCAGAAGAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAGAGCCGA
  GGTGCGGGGCGGTGGCCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGG
  CAGCATCCGCTCTCCGCTCTGGCGAGGAGGAGGTGTTGCCCATGGCCCCCGGGGCCCCAC
  AAGTTACTACTACATGCTGCCCATGAAGGTGCGGGCGCTGGGTCTCAAAGTGGCACTGAC
  CGTCAAGCTGGCCCAGGACGACCTGCACATCATGGACTCCCTAGAGCTGCCCACCGGAGA
  CCCACAGTACCTGACAGAGCTGGCGCACTACCGCCGCTGGGGGGACTCCGTACTCCTCGT
  GGACTTAACACACGAGGAGATGCCACAGAGCATCGTGGAGGCCACCTCTAGGCTTAAGAC
  CTTCAACTTGATCCCGGCTGTTGGTGAGCAAAGAGCCCAGGCCCCTAGAGTGCGCATGTG
  CAGGCTCCGCTGTTAGAATCACAGCGGTTCAAATCCGGCATCTGGTCGCTGAGTGGCCTC
  AGGCAGTGACCACGCTCCCGGACCCAACCTTCAGCTTGCCCAAAGCAATAATCTTTCCTA
  AAGAAGTGCTTGGCTGGGGATGGTGGCTCACGCTTGTAATCCCAGTACTTTGGGAGGCCA
  AGGCAGTCTGGGCAATATAGTGAGGCTCCCATCTGTACTAAAAATAAAAAAGTTAGGCGT
  GGCGATGTGCACCTGTAGTCCCAGCTACTCGGGGGCTGAGCCAGGCGGATAGCTTGAGCT
  CAGGGGGCCAAGGCTGCAGTGAGTCATGATCGCACCACTGCACCCAAACCTGGGGAGAGA
  GCTAGACTCTTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_146388.1 unedited
  GCATTGTATACGACTCCTATAGGGCGGCCGGGAATCGGCACGAGGGTCGCGTTCCCCAGT
  GTTACGGAGGGTCCTTGAGGCAGGAGTGAAAATTGGGTCTGGGGGTTAGTCCTGGGGTGG
  AGGTCTGGGCACGCCGGGTCGGACCCCCTCCATCTTCGGTTTTGCACACCCCGCTTTCCA
  GCGCGGAGTCGCGGCGGGTAGGGCGGCGTCGCGTGCGTGACGTCATCCAGCGGCGCCTCG
  CGAGGCTCCAGTGGCCTTGACCTCCCGCGGCGTGGGAGGCTGCGCGGCGATGCTGCAGTT
  CGTCCGGGCCGGGGCGCGGGCCTGGCTTCTGTCTACCGGCAGCCAGGGCCTGAGTTCCCT
  GGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTCCC
  GGAGCCCGTGCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAGGC
  CTGGGTCGAGTCCTTGCGGGGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCACCC
  CGATGTTTTCGCCACCGCGCCCAGGCTGGACATACTGCACCAGGTTGCTATGTGGCAGAA
  GAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAGAGCCGAGGTGCGGGGCGGTGG
  CCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGGCAGCATCCGCTCTCC
  GCTCTGGCGAGGAGGAGGTGTTGCCAATGGCCCCGGGGCCCAACAAGGTACTACTACATG
  CTGCCCATGAAGGTGCGGGGCGCTGGGTCTTCAAAGTGGCACTGACCGTCAAGCTGGCCA
  GGACAACCTGCACATCATGGACTCCTAAGACTGCCCACGGAAACCACAGTAACTTGACAA
  GACCTGGCGCATACGCGCTGGGGGACCTCGTACTGCCTCTGGACTTACTCTCGTGAGGAT
  TGCCA
• Restriction Sites: Please inquire
• ACCN: NM_146388
• ORF Size: 792 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_146388.1, NP_666500.1
• RefSeq Size: 2404
• RefSeq ORF: 792
• Locus ID: 51073
• Domains: Ribosomal_L4
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes an isoform (b) which has a shorter and distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.