Legumain (LGMN) (NM_001008530) Human Untagged Clone

CAT#: SC320658

LGMN (untagged)-Human legumain (LGMN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LGMN
• Synonyms: AEP; LGMN1; PRSC1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001008530.1 GAGGCTGCGAGCCGCCGCGAGTTCTCACGGTCCCGCCGGCGCCACCACCGCGGTCACTCA
  CCGCCGCCGCCGCCACCACTGCCACCACGGTCGCCTGCCACAGGTGTCTGCAATTGAACT
  CCAAGGTGCAGAATGGTTTGGAAAGTAGCTGTATTCCTCAGTGTGGCCCTGGGCATTGGT
  GCCATTCCTATAGATGATCCTGAAGATGGAGGCAAGCACTGGGTGGTGATCGTGGCAGGT
  TCAAATGGCTGGTATAATTATAGGCACCAGGCAGACGCGTGCCATGCCTACCAGATCATT
  CACCGCAATGGGATTCCTGACGAACAGATCGTTGTGATGATGTACGATGACATTGCTTAC
  TCTGAAGACAATCCCACTCCAGGAATTGTGATCAACAGGCCCAATGGCACAGATGTCTAT
  CAGGGAGTCCCGAAGGACTACACTGGAGAGGATGTTACCCCACAAAATTTCCTTGCTGTG
  TTGAGAGGCGATGCAGAAGCAGTGAAGGGCATAGGATCCGGCAAAGTCCTGAAGAGTGGC
  CCCCAGGATCACGTGTTCATTTACTTCACTGACCATGGATCTACTGGAATACTGGTTTTT
  CCCAATGAAGATCTTCATGTAAAGGACCTGAATGAGACCATCCATTACATGTACAAACAC
  AAAATGTACCGAAAGATGGTGTTCTACATTGAAGCCTGTGAGTCTGGGTCCATGATGAAC
  CACCTGCCGGATAACATCAATGTTTATGCAACTACTGCTGCCAACCCCAGAGAGTCGTCC
  TACGCCTGTTACTATGATGAGAAGAGGTCCACGTACCTGGGGGACTGGTACAGCGTCAAC
  TGGATGGAAGACTCGGACGTGGAAGATCTGACTAAAGAGACCCTGCACAAGCAGTACCAC
  CTGGTAAAATCGCACACCAACACCAGCCACGTCATGCAGTATGGAAACAAAACAATCTCC
  ACCATGAAAGTGATGCAGTTTCAGGGTATGAAACGCAAAGCCAGTTCTCCCGTCCCCCTA
  CCTCCAGTCACACACCTTGACCTCACCCCCAGCCCTGATGTGCCTCTCACCATCATGAAA
  AGGAAACTGATGAACACCAATGATCTGGAGGAGTCCAGGCAGCTCACGGAGGAGATCCAG
  CGGCATCTGGATGCCAGGCACCTCATTGAGAAGTCAGTGCGTAAGATCGTCTCCTTGCTG
  GCAGCGTCCGAGGCTGAGGTGGAGCAGCTCCTGTCCGAGAGAGCCCCGCTCACGGGGCAC
  AGCTGCTACCCAGAGGCCCTGCTGCACTTCCGGACCCACTGCTTCAACTGGCACTCCCCC
  ACGTACGAGTATGCGTTGAGACATTTGTACGTGCTGGTCAACCTTTGTGAGAAGCCGTAT
  CCACTTCACAGGATAAAATTGTCCATGGACCACGTGTGCCTTGGTCACTACTGAAGAGCT
  GCCTCCTGGAAGCTTTTCCAAGTGTGAGCGCCCCACCGACTGTGTGCTGATCAGAGACTG
  GAGAGGTGGAGTGAGAAGTCTCCGCTGCTCGGGCCCTCCTGGGGAGCCCCCGCTCCAGGG
  CTCGCTCCAGGACCTTCTTCACAAGATGACTTGCTCGCTGTTACCTGCTTCCCCAGTCTT
  TTCTGAAAAACTACAAATTAGGGTGGGAAAAGCTCTGTATTGAGAAGGGTCATATTTGCT
  TTCTAGGAGGTTTGTTGTTTTGCCTGTTAGTTTTGAGGAGCAGGAAGCTCATGGGGGCTT
  CTGTAGCCCCTCTCAAAAGGAGTCTTTATTCTGAGAATTTGAAGCTGAAACCTCTTTAAA
  TCTTCAGAATGATTTTATTGAAGAGGGCCGCAAGCCCCAAATGGAAAACTGTTTTTAGAA
  AATATGATGATTTTTGATTGCTTTTGTATTTAATTCTGCAGGTGTTCAAGTCTTAAAAAA
  TAAAGATTTATAACAGAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001008530
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008530.1, NP_001008530.1
• RefSeq Size: 2166 bp
• RefSeq ORF: 1302 bp
• Locus ID: 5641
• Cytogenetics: 14q32.12
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Antigen processing and presentation, Lysosome
• Gene Summary: 'This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) has an additional exon in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform.