RPL34 (NM_000995) Human Untagged Clone

CAT#: SC320659

RPL34 (untagged)-Human ribosomal protein L34 (RPL34), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL34
• Synonyms: L34
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000995.3 CCGAGGAGCTGAGGAAGCCTAGAGCTCTCAGAAGCAGTCCTTTGAGCTGGTGTAGGGGCA
  CTCAGAATGGTCCAGCGTTTGACATACCGACGTAGGCTTTCCTACAATACAGCCTCTAAC
  AAAACTAGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACCAAGAAGGTT
  GGGAAAGCACCAAAATCTGCATGTGGTGTGTGCCCAGGCAGACTTCGAGGGGTTCGTGCT
  GTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGCAGGGCCTAT
  GGTGGTTCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGTGCTTTCCTTATCGAG
  GAGCAGAAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGAGTCAGAAAGCTAAATAA
  AAAAATGAAACTTTTTTGAGTAATAAAAATGAAAAGACGCTGTCCAATAGAAAAAGTTGG
  TGTGCTGGAGCTACCTCACCTCAGCTTGAGAGAGCCAGTTGTGTGCATCTCTTTCCAGTT
  TTGCATCCAGTGACGTCTGCTTGGCATCTTGAGATTGTTATGGTGAGAGTATTTACACCT
  CAGCAAATGCTGCAAAATCCTGTTTTCCCCCAGAGAGCTGGAGGTTAAATACTACCAGCA
  CATCCCTAGATACTACTCAAGTTACAGTATATGATCACTAATATAGTATGCTCTTGGTAC
  CAGGAGCTCTGATATATATCTGGTACATGTTTGATAATGACTTGATTGTTATTATAAGTA
  CTTATTAATACTTCGATTCTGTAAAGAGTTTAGGGTTTGATTTTATAAAATCCAAAATGA
  GCCTTTTATTGAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000995
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000995.3, NP_000986.2
• RefSeq Size: 918 bp
• RefSeq ORF: 354 bp
• Locus ID: 6164
• Cytogenetics: 4q25
• Domains: Ribosomal_L34e
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Feb 2016]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1-6 all encode the same protein.