ESD (NM_001984) Human Untagged Clone

CAT#: SC320664

ESD (untagged)-Human esterase D (ESD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ESD
• Synonyms: FGH
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001984.1 CTTTTACTTCGGCCCGCTTCTTCTGGTCACTCCGCCACCGTAGAATCGCCTACCATTTGG
  TGCAAGCAAAAAGCAATCAGCAATTGGACAGGAAAAGAATGGCATTGAAGCAGATTTCCA
  GCAACAAGTGCTTTGGGGGATTGCAGAAAGTTTTTGAACATGACAGTGTTGAACTAAACT
  GCAAAATGAAATTTGCTGTCTACTTACCACCAAAGGCAGAAACAGGAAAGTGCCCTGCAC
  TGTATTGGCTCTCAGGTTTAACTTGCACAGAGCAAAATTTTATATCAAAATCTGGTTATC
  ATCAGTCTGCTTCAGAACATGGTCTTGTTGTCATTGCTCCAGATACCAGCCCTCGTGGCT
  GCAATATTAAAGGTGAAGATGAGAGCTGGGACTTTGGCACTGGTGCTGGATTTTATGTTG
  ATGCCACTGAAGATCCTTGGAAAACCAACTACAGAATGTACTCTTATGTCACAGAGGAGC
  TTCCCCAACTCATAAATGCCAATTTTCCAGTGGATCCCCAAAGGATGTCTATTTTTGGCC
  ACTCCATGGGAGGTCATGGAGCTCTGATCTGTGCTTTGAAAAATCCTGGAAAATACAAAT
  CTGTGTCAGCATTTGCTCCAATTTGCAACCCTGTACTCTGTCCCTGGGGCAAAAAAGCCT
  TTAGTGGATATTTGGGAACAGATCAAAGTAAATGGAAGGCTTATGATGCTACCCACCTTG
  TGAAATCCTATCCAGGATCTCAGCTGGACATACTAATTGATCAAGGGAAAGATGACCAGT
  TTCTTTTAGATGGACAGTTACTCCCTGATAACTTCATAGCTGCCTGTACAGAAAAGAAAA
  TCCCCGTTGTTTTTCGATTGCAAGAGGATTATGATCATAGCTACTACTTCATTGCAACCT
  TTATTACTGACCACATCAGACATCATGCTAAATACCTGAATGCATGAAAAAACTCCAAAT
  AAGAGAATCTCTTCAGGATTATAAAAGTTGTAAAATGCAACTGTATTGCTGAGCAAAAAA
  AAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001984
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001984.1, NP_001975.1
• RefSeq Size: 1208 bp
• RefSeq ORF: 849 bp
• Locus ID: 2098
• Cytogenetics: 13q14.2
• Gene Summary: 'This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq, Feb 2009]'