IDH3A (NM_005530) Human Untagged Clone

CAT#: SC320860

IDH3A (untagged)-Human isocitrate dehydrogenase 3 (NAD+) alpha (IDH3A), nuclear gene encoding mitochondrial protein


  "NM_005530" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol IDH3A
Synonyms H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase 3 (NAD+) a; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitric dehydrogenase; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_005530.2 CGGAGCCAGGAGGGGAAGCGATGGCTGGGCCCGCGTGGATCTCTAAGGTCTCTCGGCTGC
TGGGGGCATTCCACAACCCAAAACAGGTGACCAGAGGTTTTACTGGTGGTGTTCAGACAG
TAACTTTAATTCCAGGAGATGGTATTGGCCCAGAAATTTCAGCTGCAGTTATGAAGATTT
TTGATGCTGCCAAAGCACCTATTCAGTGGGAGGAGCGGAACGTCACTGCCATTCAAGGAC
CTGGAGGAAAGTGGATGATCCCTTCAGAGGCTAAAGAGTCCATGGATAAGAACAAGATGG
GCTTGAAAGGCCCTTTGAAGACCCCAATAGCAGCCGGTCACCCATCTATGAATTTACTGC
TGCGCAAAACATTTGACCTTTACGCGAATGTCCGACCATGTGTCTCTATCGAAGGCTATA
AAACCCCTTACACCGATGTAAATATTGTGACCATTCGAGAGAACACAGAAGGAGAATACA
GTGGAATTGAGCATGTGATTGTTGATGGAGTCGTGCAGAGTATCAAGCTCATCACCGAGG
GGGCGAGCAAGCGCATTGCTGAGTTTGCCTTTGAGTATGCCCGGAACAACCACCGGAGCA
ACGTCACGGCGGTGCACAAAGCCAACATCATGCGGATGTCAGATGGGCTTTTTCTACAAA
AATGCAGGGAAGTTGCAGAAAGCTGTAAAGATATTAAATTTAATGAGATGTACCTTGATA
CAGTATGTTTGAATATGGTACAAGATCCTTCCCAATTTGATGTTCTTGTTATGCCAAATT
TGTATGGAGACATCCTTAGTGACTTGTGTGCAGGATTGATCGGAGGTCTCGGTGTGACAC
CAAGTGGCAACATTGGAGCCAATGGGGTTGCAATTTTTGAGTCGGTTCATGGGACGGCTC
CAGACATTGCAGGCAAGGACATGGCGAATCCCACAGCCCTCCTGCTCAGTGCCGTGATGA
TGCTGCGCCACATGGGACTTTTTGACCATGCTGCAAGAATTGAGGCTGCGTGTTTTGCTA
CAATTAAGGACGGAAAGAGCTTGACAAAAGATTTGGGAGGCAATGCAAAATGCTCAGACT
TCACAGAGGAAATCTGTCGCCGAGTAAAAGATTTAGATTAACACTTCTACAACTGGCATT
TACATCAGTCACTCTAAATGGACACCACATGAACCTCTGTTTAGAATACCTACGTATGTA
TGCATTGGTTTGCTTGTTTCTTGACAGTACATTTTTAGATCTGGCCTTTTCTTAACAAAA
TCTGTGCAAAAGATGCAGGTGGATGTCCCTAGGTCTGTTTTCAAAGAACTTTTTCCAAGT
GCTTGTTTTATTTATTAAGTGTCTACCTGGTAAATGTTTTTTTTGTAAACTCTGAGTGGA
CTGTATCATTTGCTATTCTAAACCATTTTACACTTAAGTTAAAATAGTTTCTCTTCAGCT
GTAAATAACAGGATACAGAATTAACAAGAGAAAATGTCTAACTTTTTAAGAAAAACCTTA
TTTTCTTCGGTTTTTGAAAAACATAATGGAAATAAAACAGGATATTGACATAATAGCACA
AAATGACACTCTTCTAAAACTAAATGGGCACAAGAGAATTTTCCTGGGAAAGTTCACATC
AAAAAGAGTGAATGTGGTATATTTCTAAATGATATGGAAAATAGAGACAGATTTGTCCTT
TACAGAAATTACTGAGTGTGAATAAAAACTTCAGATCCAAGAAATATATAATGAGAGATA
TAATTTTTGTTAATAAGACAAAGGTAATATATTGGATACAAAGACAAAAAAAAAAAAAAA
AAA
Restriction Sites Please inquire     
ACCN NM_005530
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005530.2, NP_005521.1
RefSeq Size 2701 bp
RefSeq ORF 1101 bp
Locus ID 3419
Cytogenetics 15q25.1
Domains isodh
Protein Pathways Citrate cycle (TCA cycle), Metabolic pathways
Gene Summary 'Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]'

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