IDH3A (NM_005530) Human Untagged Clone

CAT#: SC320860

IDH3A (untagged)-Human isocitrate dehydrogenase 3 (NAD+) alpha (IDH3A), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IDH3A
• Synonyms: H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase 3 (NAD+) a; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitric dehydrogenase; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005530.2 CGGAGCCAGGAGGGGAAGCGATGGCTGGGCCCGCGTGGATCTCTAAGGTCTCTCGGCTGC
  TGGGGGCATTCCACAACCCAAAACAGGTGACCAGAGGTTTTACTGGTGGTGTTCAGACAG
  TAACTTTAATTCCAGGAGATGGTATTGGCCCAGAAATTTCAGCTGCAGTTATGAAGATTT
  TTGATGCTGCCAAAGCACCTATTCAGTGGGAGGAGCGGAACGTCACTGCCATTCAAGGAC
  CTGGAGGAAAGTGGATGATCCCTTCAGAGGCTAAAGAGTCCATGGATAAGAACAAGATGG
  GCTTGAAAGGCCCTTTGAAGACCCCAATAGCAGCCGGTCACCCATCTATGAATTTACTGC
  TGCGCAAAACATTTGACCTTTACGCGAATGTCCGACCATGTGTCTCTATCGAAGGCTATA
  AAACCCCTTACACCGATGTAAATATTGTGACCATTCGAGAGAACACAGAAGGAGAATACA
  GTGGAATTGAGCATGTGATTGTTGATGGAGTCGTGCAGAGTATCAAGCTCATCACCGAGG
  GGGCGAGCAAGCGCATTGCTGAGTTTGCCTTTGAGTATGCCCGGAACAACCACCGGAGCA
  ACGTCACGGCGGTGCACAAAGCCAACATCATGCGGATGTCAGATGGGCTTTTTCTACAAA
  AATGCAGGGAAGTTGCAGAAAGCTGTAAAGATATTAAATTTAATGAGATGTACCTTGATA
  CAGTATGTTTGAATATGGTACAAGATCCTTCCCAATTTGATGTTCTTGTTATGCCAAATT
  TGTATGGAGACATCCTTAGTGACTTGTGTGCAGGATTGATCGGAGGTCTCGGTGTGACAC
  CAAGTGGCAACATTGGAGCCAATGGGGTTGCAATTTTTGAGTCGGTTCATGGGACGGCTC
  CAGACATTGCAGGCAAGGACATGGCGAATCCCACAGCCCTCCTGCTCAGTGCCGTGATGA
  TGCTGCGCCACATGGGACTTTTTGACCATGCTGCAAGAATTGAGGCTGCGTGTTTTGCTA
  CAATTAAGGACGGAAAGAGCTTGACAAAAGATTTGGGAGGCAATGCAAAATGCTCAGACT
  TCACAGAGGAAATCTGTCGCCGAGTAAAAGATTTAGATTAACACTTCTACAACTGGCATT
  TACATCAGTCACTCTAAATGGACACCACATGAACCTCTGTTTAGAATACCTACGTATGTA
  TGCATTGGTTTGCTTGTTTCTTGACAGTACATTTTTAGATCTGGCCTTTTCTTAACAAAA
  TCTGTGCAAAAGATGCAGGTGGATGTCCCTAGGTCTGTTTTCAAAGAACTTTTTCCAAGT
  GCTTGTTTTATTTATTAAGTGTCTACCTGGTAAATGTTTTTTTTGTAAACTCTGAGTGGA
  CTGTATCATTTGCTATTCTAAACCATTTTACACTTAAGTTAAAATAGTTTCTCTTCAGCT
  GTAAATAACAGGATACAGAATTAACAAGAGAAAATGTCTAACTTTTTAAGAAAAACCTTA
  TTTTCTTCGGTTTTTGAAAAACATAATGGAAATAAAACAGGATATTGACATAATAGCACA
  AAATGACACTCTTCTAAAACTAAATGGGCACAAGAGAATTTTCCTGGGAAAGTTCACATC
  AAAAAGAGTGAATGTGGTATATTTCTAAATGATATGGAAAATAGAGACAGATTTGTCCTT
  TACAGAAATTACTGAGTGTGAATAAAAACTTCAGATCCAAGAAATATATAATGAGAGATA
  TAATTTTTGTTAATAAGACAAAGGTAATATATTGGATACAAAGACAAAAAAAAAAAAAAA
  AAA
• Restriction Sites: Please inquire
• ACCN: NM_005530
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005530.2, NP_005521.1
• RefSeq Size: 2701 bp
• RefSeq ORF: 1101 bp
• Locus ID: 3419
• Cytogenetics: 15q25.1
• Domains: isodh
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways
• Gene Summary: 'Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]'