SC5DL (SC5D) (NM_001024956) Human Untagged Clone

CAT#: SC320865

SC5DL (untagged)-Human sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like (SC5DL), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SC5D
• Synonyms: ERG3; S5DES; SC5DL
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001024956.2 AGAGACTTCAGCGCCTGGGACTCGGGTGGGCGAGGCGGAAGGTGTCCTCGCAGCACGGCT
  TTTCTCCGCGCCGCGGTTGGTTAGCGAGTGCCCTCTGGGTGCTAGGCGTTGGGCGGATGG
  TAGGATCGCGGTAGCATACGGATCCGAGTCCTGCGCCGAGTGAGAGGAGAGGCTGGCAGG
  GGCTAAGTGATGGATCTTGTACTCCGTGTTGCAGATTACTATTTTTTTACACCATACGTG
  TATCCAGCCACATGGCCAGAAGATGACATCTTCCGACAAGCTATTAGTCTTCTGATTGTA
  ACAAATGTTGGTGCTTACATCCTTTATTTCTTCTGTGCAACACTGAGCTATTATTTTGTC
  TTCGATCATGCATTAATGAAACATCCACAATTTTTAAAGAATCAAGTCCGTCGAGAGATT
  AAGTTTACTGTCCAGGCATTGCCATGGATAAGTATTCTTACTGTTGCACTGTTCTTGCTG
  GAGATAAGAGGTTACAGCAAATTACATGATGACCTAGGAGAGTTTCCATATGGATTGTTT
  GAACTTGTCGTTAGTATAATATCTTTCCTCTTTTTCACTGACATGTTCATCTACTGGATT
  CACAGAGGCCTTCATCATAGACTGGTATATAAGCGCCTACATAAACCTCACCATATTTGG
  AAGATTCCTACTCCATTTGCAAGTCATGCTTTTCACCCTATTGATGGCTTTCTTCAGAGT
  CTACCTTACCATATATACCCTTTTATCTTTCCATTACACAAGGTGGTTTATTTAAGTCTG
  TACATCTTGGTTAATATCTGGACAATTTCCATTCATGACGGTGATTTTCGTGTCCCCCAA
  ATCTTACAGCCATTTATTAATGGCTCAGCTCATCATACAGACCACCATATGTTCTTTGAC
  TATAATTATGGACAATATTTCACTTTGTGGGATAGGATTGGCGGCTCATTCAAAAATCCT
  TCATCCTTTGAGGGGAAGGGACCGCTCAGTTATGTGAAGGAGATGACAGAGGGAAAGCGC
  AGCAGCCATTCAGGAAATGGCTGTAAGAATGAAAAATTATTCAATGGAGAGTTTACAAAG
  ACTGAATAGATTATTGCCCAGTTATTCTTAAGTAAGGACAAAGAAGGAAATATCATCGTA
  TTTCTTTTTTTTAATAAGGAAAAAATAATATCCATACAGTCAAGATACATAGTAAATGGT
  ATCATTTGGAAATCAGCATCGTGGGCACTGCTGAGGAATGATCCTAGTGGTAGGTCAGAA
  GAAGATGCTGTGAACACCAGGACTTTAATCTTATGCTTAAAATGCCAGATGTTGTTCGGG
  GGACAACTTGTATCTTTCTAGCAGCAGATCTGTAGTTTGTATAGCCTCAACAACAATTTT
  AAATAAGATGGAGAATAAATTATTGAGGGGACTAGGCTATATGCATTTGCCTTCATCCAC
  CCATGTTTATTAAGAATCATTGTGCTTAATAATACCAAGACTAAGCACCATAACCAAGAA
  ATACTAATGTAAAGATTGTTTCTTGTTTCAGGAATGGTTAATTCTTCAACGTTGGTATGA
  TAATGATAACTTGTTTTGACTTGAATAAAGTACTACATCAGTGTGGAAAAAAATTCTGAT
  ACATTAGCAGCTATGTAAATGACCTAATTGATAGCAGGTGTAATAAGACTATCGTCTTCC
  TACACATAGGAGGCTCATTCTCTGGACACACTATCACCTATTACATTTTACTGATTAACA
  AATAAATTGGAATTTAAAAATATCGATATCACCATGATTTAATCCAGATCTGGGATTATG
  TAGCTAAACATTGTGATGATTATTATTTAAAACCATTATTTAATAAGAGTAAAAATATGT
  GAATCTGGATATATTTAAAAAAAGAAATTTGATGCCCAGATAATATATTAGGCACTACTG
  ATTTTTTAGTTAAATTGATGCACTACACTTTTGATGTTTGAAGTTACAAACCTGTAATTT
  TTTTGTAAAGGAAATAATTGCCAAATACCTAGGCCCATTGCTGACGATTAGTTCTAAAAT
  CTTATTCCTCCTCTTCTCCCCTCACTTTTCCCTACTTCCTCTGCAAAAAGATTTAACAAA
  TACATTCATAAGGAAATGTGTGTTGTAACAAATATATTGCAAAAACATAGTTTGTAAAGG
  CATTCTATAAGCTATTTATGTAAAATCAATAAAAGTTGATCATAATTAAAAAAAAAAAAA
  AAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001024956
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024956.2, NP_001020127.1
• RefSeq Size: 7019 bp
• RefSeq ORF: 900 bp
• Locus ID: 6309
• Cytogenetics: 11q23.3-q24.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: 'This gene encodes an enzyme of cholesterol biosynthesis. The encoded protein catalyzes the conversion of lathosterol into 7-dehydrocholesterol. Mutations in this gene have been associated with lathosterolosis. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate 5' exon compared to variant 1. Variants 1 and 2 encode the same protein.