NASP (NM_002482) Human Untagged Clone

CAT#: SC320902

NASP (untagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NASP
• Synonyms: FLB7527; HMDRA1; PRO1999
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002482.2 GTCCCTGAGTGAGTCTCTGGCGTCCCAAATTGCCTGTTTTTCTCGCAGGCTCTATTCCGT
  TCGCTGGTTCGCCACCTCAGGGGAACGATGGCCATGGAGTCCACAGCCACTGCCGCCGTC
  GCCGCGGAGCTGGTTTCTGCCGACAAAATTGAAGATGTTCCTGCTCCTTCTACATCTGCA
  GATAAAGTGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTATTGGGTTTAGGACAG
  AAACATCTGGTGATGGGGGATATTCCAGCAGCTGTCAATGCATTCCAGGAAGCAGCTAGT
  CTTTTAGGTAAGAAGTATGGAGAGACAGCTAATGAGTGTGGAGAAGCCTTCTTTTTCTAT
  GGGAAATCACTTCTGGAGTTGGCAAGAATGGAGAATGGTGTGTTGGGAAACGCCTTGGAA
  GGTGTGCATGTGGAAGAGGAAGAAGGAGAAAAAACAGAAGATGAATCTCTGGTAGAAAAT
  AATGATAACATAGATGAGGAAGCAAGGGAAGAGTTGAGAGAACAGGTTTATGACGCCATG
  GGAGAAAAAGAAGAAGCCAAAAAAACAGAAGACAAGTCTTTGGCAAAGCCTGAAACTGAT
  AAAGAACAGGACAGTGAAATGGAGAAGGGTGGAAGAGAAGATATGGATATAAGTAAATCT
  GCAGAGGAGCCACAGGAAAAAGTTGACTTGACTCTAGATTGGTTAACTGAAACCTCTGAA
  GAGGCAAAAGGAGGAGCAGCACCAGAAGGACCGAATGAAGCTGAGGTCACTTCTGGGAAG
  CCAGAACAGGAAGTACCAGATGCTGAGGAAGAAAAATCAGTTTCTGGAACTGATGTCCAA
  GAAGAGTGCAGAGAAAAAGGAGGTCAGGAGAAGCAGGGAGAGGTAATTGTGAGCATAGAG
  GAGAAGCCAAAAGAAGTTTCAGAAGAGCAGCCTGTGGTGACTCTAGAAAAGCAGGGCACT
  GCAGTGGAGGTAGAAGCAGAGTCTTTAGACCCGACAGTCAAGCCAGTGGATGTGGGTGGG
  GACGAGCCAGAGGAGAAGGTAGTTACCTCTGAAAACGAGGCAGGAAAGGCGGTTCTTGAA
  CAACTGGTAGGTCAAGAAGTACCACCTGCTGAAGAGTCACCAGAGGTGACAACAGAGGCT
  GCAGAGGCCTCAGCTGTAGAGGCTGGATCAGAAGTCTCTGAAAAGCCTGGGCAGGAGGCT
  CCAGTTCTCCCTAAGGATGGTGCAGTCAATGGACCGTCAGTTGTAGGAGATCAGACTCCT
  ATTGAACCACAGACTTCTATAGAAAGACTGACAGAAACAAAAGATGGCTCAGGACTAGAG
  GAGAAGGTCAGGGCAAAGCTGGTTCCTAGTCAGGAGGAGACTAAGCTGTCTGTAGAAGAG
  TCTGAGGCAGCTGGAGATGGGGTTGATACCAAGGTAGCCCAGGGAGCTACTGAGAAATCA
  CCTGAAGACAAAGTTCAGATAGCTGCTAATGAAGAGACACAAGAGAGAGAAGAACAGATG
  AAAGAGGGTGAAGAAACTGAAGGCTCAGAAGAGGATGATAAAGAAAATGATAAGACCGAA
  GAAATGCCAAATGATTCAGTCCTTGAAAACAAGTCTCTTCAAGAAAATGAGGAGGAGGAG
  ATTGGGAACCTAGAGCTTGCCTGGGATATGCTGGATTTAGCAAAGATCATTTTTAAAAGG
  CAAGAAACAAAAGAAGCACAGCTTTATGCTGCCCAGGCACATCTTAAACTCGGAGAAGTT
  AGTGTTGAATCTGAAAACTATGTGCAAGCTGTGGAGGAGTTCCAGTCCTGCCTTAACCTG
  CAGGAACAGTACCTGGAAGCCCACGACCGTCTCCTTGCAGAGACCCACTACCAGCTGGGC
  TTGGCTTATGGGTACAACTCTCAGTATGATGAGGCAGTGGCACAGTTCAGCAAATCTATT
  GAAGTCATTGAGAACAGAATGGCTGTACTAAACGAGCAGGTGAAGGAGGCTGAAGGATCG
  TCTGCTGAATACAAGAAAGAAATTGAGGAACTAAAGGAACTGCTACCCGAAATTAGAGAG
  AAGATAGAAGATGCAAAGGAGTCTCAGCGTAGTGGGAATGTAGCTGAACTGGCTCTGAAA
  GCTACTCTGGTGGAGAGTTCTACTTCAGGTTTCACTCCTGGTGGAGGAGGCTCTTCAGTC
  TCCATGATTGCCAGTAGAAAGCCAACAGACGGTGCTTCCTCATCAAATTGTGTGACTGAT
  ATTTCCCACCTTGTCAGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAAGATGAT
  GCAAAGAAAGCCAAACAAGAGCCGGAGGTGAACGGAGGCAGTGGGGATGCTGTCCCCAGT
  GGAAATGAAGTTTCGGAAAACATGGAGGAGGAGGCTGAGAATCAGGCTGAAAGCCGGGCA
  GCAGTGGAGGGGACAGTGGAGGCTGGAGCTACAGTTGAAAGCACTGCATGTTAAGAGGGG
  GCACAGCCCTCCTCCCAAGGGAAAGTGTTTTTGTATATAATGTATTTTTTCACTTTTGGA
  GGATTCTTTTTGTATAACTTCAATAAAGATTGTAAGCAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002482
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002482.2, NP_002473.2
• RefSeq Size: 3323 bp
• RefSeq ORF: 2367 bp
• Locus ID: 4678
• Cytogenetics: 1p34.1
• Domains: TPR
• Gene Summary: 'This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).