GBE1 (NM_000158) Human Untagged Clone

CAT#: SC320912

GBE1 (untagged)-Human glucan (1,4-alpha-), branching enzyme 1 (GBE1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GBE1
• Synonyms: APBD; GBE; GSD4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000158.2 GCTCCTCCCCGCTGGCGGGGGGAGAAAGGGCAGGAGGCCTTCCGTCCCGGCTATAAAGGG
  CCCCGGACCGCCGCGGCTCGCCTCGGCTTGCCTCGACACGCCTAGGCGCCCTCCGGCTCC
  GCCCTAGCCGCCGCGTCCCAGCTAGAGCTCCAGCGCCCGCTCAGGCCCCACTCGACCCTC
  TCGGGCCTCGGCTACTTGGACTGCGGCGGAATATGGCGGCTCCGATGACTCCCGCGGCTC
  GGCCCGAGGACTACGAGGCGGCGCTCAATGCCGCCCTGGCTGACGTGCCCGAACTGGCCA
  GACTCCTGGAGATCGACCCGTACTTGAAGCCCTACGCCGTGGACTTCCAGCGCAGGTATA
  AGCAGTTTAGCCAAATTTTGAAGAACATTGGAGAAAATGAAGGTGGTATTGATAAGTTTT
  CCAGAGGCTATGAATCATTTGGCGTCCACAGATGTGCTGATGGTGGTTTATACTGCAAAG
  AATGGGCCCCGGGAGCAGAAGGAGTTTTTCTTACTGGAGATTTTAATGGTTGGAATCCAT
  TTTCGTACCCATACAAAAAACTGGATTATGGAAAATGGGAGCTGTATATCCCACCAAAGC
  AGAATAAATCTGTACTCGTGCCTCATGGATCCAAATTAAAGGTAGTTATTACTAGTAAAA
  GCGGAGAGATCTTGTATCGTATTTCACCGTGGGCAAAGTATGTGGTTCGTGAAGGTGATA
  ATGTGAATTATGATTGGATACACTGGGATCCAGAACACTCATATGAGTTTAAGCATTCCA
  GACCAAAGAAGCCACGGAGTCTAAGAATTTATGAATCTCATGTGGGAATTTCTTCCCATG
  AAGGAAAAGTAGCTTCTTATAAACATTTTACATGCAATGTACTACCAAGAATCAAAGGCC
  TTGGATACAACTGCATTCAGTTGATGGCAATCATGGAGCATGCTTACTATGCCAGCTTTG
  GTTACCAAATCACAAGCTTCTTTGCAGCTTCCAGCCGTTATGGATCACCTGAAGAGCTAC
  AAGAACTGGTAGACACAGCTCATTCCATGGGTATCATAGTCCTCTTAGATGTGGTACACA
  GCCATGCTTCAAAAAATTCAGCAGATGGATTGAATATGTTTGATGGGACAGATTCCTGTT
  ATTTTCATTCTGGACCTAGAGGGACTCATGATCTTTGGGATAGCAGATTGTTTGCCTACT
  CCAGCTGGGAAGTTTTAAGATTCCTTCTGTCAAACATAAGATGGTGGTTGGAAGAATATC
  GCTTTGATGGATTTCGTTTTGATGGTGTTACGTCCATGCTTTATCATCACCATGGAGTGG
  GTCAAGGTTTCTCAGGTGATTACAGTGAATATTTCGGACTACAAGTAGATGAAGATGCCT
  TGACTTACCTCATGTTGGCAAATCATTTGGTTCACACGCTGTGTCCCGATTCTATAACAA
  TAGCTGAGGATGTATCAGGAATGCCAGCTCTGTGCTCTCCAATTTCCCAGGGAGGGGGTG
  GTTTTGACTATCGACTAGCCATGGCAATTCCAGATAAGTGGATTCAGCTACTTAAAGAGT
  TTAAAGATGAAGACTGGAACATGGGCGATATAGTATACACGCTCACAAACAGGCGCTACC
  TTGAAAAGTGCATTGCTTATGCAGAGAGCCATGATCAGGCATTGGTTGGGGATAAGTCGC
  TGGCATTTTGGTTGATGGATGCCGAAATGTATACAAACATGAGTGTCCTGACTCCTTTTA
  CTCCAGTTATTGATCGTGGAATACAGCTTCATAAAATGATTCGACTCATTACGCATGGGC
  TTGGTGGAGAAGGCTATCTCAATTTCATGGGTAATGAATTTGGGCATCCTGAATGGTTAG
  ACTTCCCAAGAAAAGGAAATAATGAGAGTTACCATTATGCCAGGCGGCAGTTTCATTTAA
  CTGACGACGACCTTCTTCGCTACAAGTTCCTAAATAATTTTGACAGGGATATGAATAGAT
  TGGAAGAAAGATATGGTTGGCTTGCAGCTCCACAGGCCTACGTGAGTGAAAAACATGAAG
  GCAATAAGATCATTGCTTTTGAAAGAGCAGGTCTTCTTTTCATTTTCAACTTCCATCCAA
  GCAAGAGCTACACTGACTACCGAGTTGGAACAGCATTGCCAGGGAAATTCAAAATTGTGC
  TAGATTCAGATGCAGCGGAATATGGAGGGCATCAGAGACTGGACCACAGCACTGACTTTT
  TTTCTGAGGCTTTTGAACATAATGGGCGTCCCTATTCTCTTTTGGTGTACATTCCAAGCA
  GAGTGGCCCTCATCCTTCAGAATGTGGATCTGCCGAATTGAAGAGGCCTGATTTCAGCTC
  CACCAGATGCAGATTTGTGTTTTGTTTTCTTGTTATCACTGTCACACAGCTTATAACATG
  TATGCTTTTCAGAATACAGTTGTCTAGCCAAGCCATCAAGTGTCTGAAATTCAATATTGG
  TTTATGCAAATACAGCAAACTTTTATTTAAGTAGATAGGAGAATATGTTTAAAATATTAG
  GAATCCTAGACCATATTTTCAAGTCATCTTAGCAGCTAGGATTCTCAAATGGAAGTGTTA
  TATATAATATGTTAAAAACATTTTGCTTTCCTGGCTAATTATTTGATCCTTTTAAATTCA
  AATTTGAATCATTTGTCATGTATGATTATTTCTGTTAAATGTACACAGTATTTAAGATGG
  ATATTTGGTGGCTCTATTTGTTCTGATATCTTTTGGTCTAAATTATGAGGTACCAAGATT
  GTTTCTTTGTTTCTTTTTTTCAAATTGTGTTTAGAAATACTGTAATAAATATGCAGTAGT
  GATATAAAGAATTATATCCAAGGTAATATAAAAGCCATTACGTATGAACTCAAAAAAAAA
  AAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000158
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000158.2, NP_000149.2
• RefSeq Size: 3099 bp
• RefSeq ORF: 2109 bp
• Locus ID: 2632
• Cytogenetics: 3p12.2
• Domains: isoamylase_N, alpha-amylase, Aamy
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism
• Gene Summary: 'The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq, Jul 2008]'