Fibrinogen beta chain (FGB) (NM_005141) Human Untagged Clone

CAT#: SC321032

FGB (untagged)-Human fibrinogen beta chain (FGB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGB
• Synonyms: HEL-S-78p
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005141.2 GGGGCTACATGAAAAGGATGGTTTCTTGGAGCTTCCACAAACTTAAAACCATGAAACATC
  TATTATTGCTACTATTGTGTGTTTTTTTAGTTAAGTCCCAAGGTGTCAACGACAATGAGG
  AGGGTTTCTTCAGTGCCCGTGGTCATCGACCCCTTGACAAGAAGAGAGAAGAGGCTCCCA
  GCCTGAGGCCTGCCCCACCGCCCATCAGTGGAGGTGGCTATCGGGCTCGTCCAGCCAAAG
  CAGCTGCCACTCAAAAGAAAGTAGAAAGAAAAGCCCCTGATGCTGGAGGCTGTCTTCACG
  CTGACCCAGACCTGGGGGTGTTGTGTCCTACAGGATGTCAGTTGCAAGAGGCTTTGCTAC
  AACAGGAAAGGCCAATCAGAAATAGTGTTGATGAGTTAAATAACAATGTGGAAGCTGTTT
  CCCAGACCTCCTCTTCTTCCTTTCAGTACATGTATTTGCTGAAAGACCTGTGGCAAAAGA
  GGCAGAAGCAAGTAAAAGATAATGAAAATGTAGTCAATGAGTACTCCTCAGAACTGGAAA
  AGCACCAATTATATATAGATGAGACTGTGAATAGCAATATCCCAACTAACCTTCGTGTGC
  TTCGTTCAATCCTGGAAAACCTGAGAAGCAAAATACAAAAGTTAGAATCTGATGTCTCAG
  CTCAAATGGAATATTGTCGCACCCCATGCACTGTCAGTTGCAATATTCCTGTGGTGTCTG
  GCAAAGAATGTGAGGAAATTACCAGGAAAGGAGGTGAAACATCTGAAATGTATCTCATTC
  AACCTGACAGTTCTGTCAAACCGTATAGAGTATACTGTGACATGAATACAGAAAATGGAG
  GATGGACAGTGATTCAGAACCGTCAAGACGGTAGTGTTGACTTTGGCAGGAAATGGGATC
  CATATAAACAGGGATTTGGAAATGTTGCAACCAACACAGATGGGAAGAATTACTGTGGCC
  TACCAGGTGAATATTGGCTTGGAAATGATAAAATTAGCCAGCTTACCAGGATGGGACCCA
  CAGAACTTTTGATAGAAATGGAGGACTGGAAAGGAGACAAAGTAAAGGCTCACTATGGAG
  GATTCACTGTACAGAATGAAGCCAACAAATACCAGATCTCAGTGAACAAATACAGAGGAA
  CAGCCGGTAATGCCCTCATGGATGGAGCATCTCAGCTGATGGGAGAAAACAGGACCATGA
  CCATTCACAACGGCATGTTCTTCAGCACGTATGACAGAGACAATGACGGCTGGTTAACAT
  CAGATCCCAGAAAACAGTGTTCTAAAGAAGACGGTGGTGGATGGTGGTATAATAGATGTC
  ATGCAGCCAATCCAAACGGCAGATACTACTGGGGTGGACAGTACACCTGGGACATGGCAA
  AGCATGGCACAGATGATGGTGTAGTATGGATGAATTGGAAGGGGTCATGGTACTCAATGA
  GGAAGATGAGTATGAAGATCAGGCCCTTCTTCCCACAGCAATAGTCCCCAATACGTAGAT
  TTTTGCTCTTCTGTATGTGACAACATTTTTGTACATTATGTTATTGGAATTTTCTTTCAT
  ACATTATATTCCTCTAAAACTCTCAAGCAGACGTGAGTGTGACTTTTTGAAAAAAGTATA
  GGATAAATTACATTAAAATAGCACATGATTTTCTTTTGTTTTCTTCATTTCTCTTGCTCA
  CCAAGAAGTAACAAAAGTATAGTTTTGACAGAGTTGGTGTTCATAATTTCAGTTCTAGTT
  GATTGCGAGAATTTTCAAATAAGGAAGAGGGGTCTTTTATCCTTGTCGTAGGAAAACCAT
  GACGGAAAGGAAAAACTGATGTTTAAAAGTCCACTTTTAAAACTATATTTATTTATGTAG
  GATCTGTCAAAGAAAACTTCCAAAAAGATTTATTAATTAAACCAGCCTCTGTTGCAATAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005141
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005141.2, NP_005132.2
• RefSeq Size: 1949 bp
• RefSeq ORF: 1476 bp
• Locus ID: 2244
• Cytogenetics: 4q31.3
• Domains: FBG
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: 'The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Fibrinogen serves key roles in hemostasis and antimicrobial host defense. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.