EIF4A1 (NM_001416) Human Untagged Clone

CAT#: SC321083

EIF4A1 (untagged)-Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF4A1
• Synonyms: DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001416.2 AGTTTCTAAGGATCATGTCTGCGAGCCAGGATTCCCGATCCAGAGACAATGGCCCCGATG
  GGATGGAGCCCGAAGGCGTCATCGAGAGTAACTGGAATGAGATTGTTGACAGCTTTGATG
  ACATGAACCTCTCGGAGTCCCTTCTCCGTGGCATCTACGCCTATGGTTTTGAGAAGCCCT
  CTGCCATCCAGCAGCGAGCCATTCTACCTTGTATCAAGGGTTATGATGTGATTGCTCAAG
  CCCAATCTGGGACTGGGAAAACGGCCACATTTGCCATATCAATTCTGCAGCAGATTGAAT
  TAGATCTAAAAGCCACCCAGGCCTTGGTCCTAGCACCCACTCGAGAATTGGCTCAGCAGA
  TACAGAAGGTGGTCATGGCACTAGGAGACTACATGGGCGCCTCCTGTCACGCCTGTATCG
  GGGGCACCAACGTGCGTGCTGAGGTGCAGAAACTGCAGATGGAAGCTCCCCACATCATCG
  TGGGTACCCCTGGCCGTGTGTTTGATATGCTTAACCGGAGATACCTGTCCCCCAAATACA
  TCAAGATGTTTGTACTGGATGAAGCTGACGAAATGTTAAGCCGTGGATTCAAGGACCAGA
  TCTATGACATATTCCAAAAGCTCAACAGCAACACCCAGGTAGTTTTGCTGTCAGCCACAA
  TGCCTTCTGATGTGCTTGAGGTGACCAAGAAGTTCATGAGGGACCCCATTCGGATTCTTG
  TCAAGAAGGAAGAGTTGACCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAG
  AGGAGTGGAAGCTGGACACACTATGTGACTTGTATGAAACCCTGACCATCACCCAGGCAG
  TCATCTTCATCAACACCCGGAGGAAGGTGGACTGGCTCACCGAGAAGATGCATGCTCGAG
  ATTTCACTGTATCCGCCATGCATGGAGATATGGACCAAAAGGAACGAGACGTGATTATGA
  GGGAGTTTCGTTCTGGCTCTAGCAGAGTTTTGATTACCACTGACCTGCTGGCCAGAGGCA
  TTGATGTGCAGCAGGTTTCTTTAGTCATCAACTATGACCTTCCCACCAACAGGGAAAACT
  ATATCCACAGAATCGGTCGAGGTGGACGGTTTGGCCGTAAAGGTGTGGCTATTAACATGG
  TGACAGAAGAAGACAAGAGGACTCTTCGAGACATTGAGACCTTCTACAACACCTCCATTG
  AGGAAATGCCCCTCAATGTTGCTGACCTCATCTGAGGGGCTGTCCTGCCACCCAGCCCCA
  GCCAGGGCTCAATCTCTGGGGGCTGAGGAGCAGCAGGAGGGGGGAGGGAAGGGAGCCAAG
  GGATGGACATCTTGTCATTTTTTTTCTTTGAATAAATGTCACTTTTTGAGGCAAAAGAAG
  GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001416
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001416.2, NP_001407.1
• RefSeq Size: 1844 bp
• RefSeq ORF: 1221 bp
• Locus ID: 1973
• Cytogenetics: 17p13.1
• Domains: DEAD, helicase_C
• Gene Summary: ''
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).