HCLS1 (NM_005335) Human Untagged Clone

CAT#: SC321204

HCLS1 (untagged)-Human hematopoietic cell-specific Lyn substrate 1 (HCLS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HCLS1
• Synonyms: CTTNL; HS1; lckBP1; p75
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005335.3 GTGGACGCGAGGAGCCGGGCGCTTAGAACAGAGGCTTGCACAGGTGGAGATGTGGAAGTC
  TGTAGTGGGCCATGATGTGTCTGTTTCCGTGGAGACCCAGGGTGATGATTGGGACACAGA
  TCCTGACTTTGTGAATGACATCTCTGAAAAGGAGCAACGATGGGGAGCCAAGACCATCGA
  GGGGTCTGGACGCACAGAACACATCAACATCCACCAGCTGAGGAACAAAGTATCAGAGGA
  GCATGATGTTCTCAGGAAGAAAGAGATGGAGTCAGGGCCCAAAGCATCCCATGGCTATGG
  AGGTCGGTTTGGAGTAGAAAGAGACCGAATGGACAAGAGTGCAGTGGGCCATGAGTATGT
  TGCCGAGGTGGAGAAGCACTCTTCTCAGACGGATGCTGCCAAAGGCTTTGGGGGCAAGTA
  CGGAGTTGAGAGGGACAGGGCAGACAAGTCAGCAGTCGGCTTTGATTATAAAGGAGAAGT
  GGAGAAGCATACATCTCAGAAAGATTACTCTCGTGGCTTTGGTGGCCGGTACGGGGTGGA
  GAAGGATAAATGGGACAAAGCAGCTCTGGGATATGACTACAAGGGAGAGACGGAGAAACA
  CGAGTCCCAGAGAGATTATGCCAAGGGCTTTGGTGGCCAGTATGGAATCCAGAAGGACCG
  AGTGGATAAGAGCGCTGTCGGCTTCAATGAAATGGAGGCCCCGACCACAGCTTATAAGAA
  GACGACGCCCATAGAAGCCGCTTCTAGTGGTGCCCGTGGGCTGAAGGCGAAATTTGAGTC
  CATGGCTGAGGAGAAGAGGAAGCGAGAGGAAGAGGAGAAGGCACAGCAGGTGGCCAGGAG
  GCAACAGGAGCGAAAGGCTGTGACAAAGAGGAGCCCTGAGGCTCCACAGCCAGTGATAGC
  TATGGAAGAGCCAGCAGTACCGGCCCCACTGCCCAAGAAAATCTCCTCAGAGGCCTGGCC
  TCCAGTTGGGACTCCTCCATCATCAGAGTCTGAGCCTGTGAGAACCAGCAGGGAACACCC
  AGTGCCCTTGCTGCCCATTAGGCAGACTCTCCCGGAGGACAATGAGGAGCCCCCAGCTCT
  GCCCCCTAGGACTCTGGAAGGCCTCCAGGTGGAGGAAGAGCCAGTGTACGAAGCAGAGCC
  TGAGCCTGAGCCCGAGCCTGAGCCCGAGCCTGAGAATGACTATGAGGACGTTGAGGAGAT
  GGACAGGCATGAGCAGGAGGATGAACCAGAGGGGGACTATGAGGAGGTGCTCGAGCCTGA
  AGATTCTTCTTTTTCTTCTGCTCTGGCTGGATCATCAGGCTGCCCGGCTGGGGCTGGGGC
  TGGGGCTGTGGCTCTGGGGATCTCAGCTGTGGCTCTATATGATTACCAAGGAGAGGGAAG
  TGATGAGCTTTCCTTTGATCCGGACGACGTAATCACTGACATTGAGATGGTGGACGAGGG
  CTGGTGGCGGGGACGTTGCCATGGCCACTTTGGACTCTTCCCTGCAAATTATGTCAAGCT
  TCTGGAGTGACTAGAGCTCACTGTCTACTGCAACTGTGATTTCCCATGTCCAAAGTGGCT
  CTGCCTCCACCCCCTCCCTATTCCTGATGCAAATGTCTAACCAGATGAGTTTCTGGACAG
  ACTTCCCTCTCCTGCTTCATTAAGGGCTTGGGGCAGAGACAGCATGGGGAAGGAGGTCCC
  CTTCCCCAAGAGTCCTCTCTATCCTGGATGAGCTCATGAACATTTCTCTTGTGTTCCTGA
  CTCCTTCCCAATGAACACCTCTCTGCCACCCCAAGCTCTGCTCTCCTCCTCTGTGAGCTC
  TGGGCTTCCCAGTTTGTTTACCCGGGAAAGTACGTCTAGATTGTGTGGTTTGCCTCATTG
  TGCTATTTGCCCACTTTCCTTCCCTGAAGAAATATCTGTGAACCTTCTTTCTGTTCAGTC
  CTAAAATTCGAAATAAAGTGAGACTATGGTTCACCTGTAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005335
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005335.3, NP_005326.1
• RefSeq Size: 2029 bp
• RefSeq ORF: 1461 bp
• Locus ID: 3059
• Cytogenetics: 3q13.33
• Domains: SH3, HS1_rep
• Protein Families: Transcription Factors
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction
• Gene Summary: ''
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).