S4 (PSMC1) (NM_002802) Human Untagged Clone

CAT#: SC321319

PSMC1 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 1 (PSMC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMC1
• Synonyms: P26S4; p56; S4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002802.2 TTCCGGCAGCGGCAGCTCAAGTGGCCAAGGCAAGATGGGTCAAAGTCAGAGTGGTGGTCA
  TGGTCCTGGAGGTGGCAAGAAGGATGACAAGGACAAGAAAAAGAAATATGAACCTCCTGT
  ACCAACTAGAGTGGGGAAAAAGAAGAAGAAAACAAAGGGACCAGATGCTGCCAGCAAACT
  GCCACTGGTGACACCTCACACTCAGTGCCGGTTAAAATTACTGAAGTTAGAGAGAATTAA
  AGACTATCTTCTCATGGAGGAAGAATTCATTAGAAATCAGGAACAAATGAAACCATTAGA
  AGAAAAGCAAGAGGAGGAAAGATCAAAAGTGGATGATCTGAGGGGGACCCCGATGTCAGT
  AGGAACCTTGGAAGAGATTATTGATGACAATCATGCCATCGTGTCTACATCTGTGGGCTC
  AGAACACTACGTCAGCATTCTTTCATTTGTAGACAAGGATCTGCTGGAACCTGGCTGCTC
  GGTCCTGCTCAACCACAAGGTGCATGCCGTGATAGGGGTGCTGATGGATGACACGGATCC
  CCTGGTCACAGTGATGAAGGTAGAAAAGGCCCCCCAGGAGACCTATGCAGATATTGGGGG
  GTTGGACAACCAAATTCAGGAAATTAAGGAATCTGTGGAGCTTCCTCTCACCCATCCTGA
  ATATTATGAAGAGATGGGTATAAAGCCTCCTAAGGGGGTCATTCTCTATGGTCCACCTGG
  CACAGGTAAAACCTTGTTAGCCAAAGCAGTAGCAAACCAAACCTCAGCCACTTTCTTGAG
  AGTGGTTGGCTCTGAACTTATTCAGAAGTACCTAGGTGATGGGCCCAAACTCGTACGGGA
  ATTGTTCCGAGTTGCTGAAGAACATGCACCGTCCATCGTGTTTATTGATGAAATTGACGC
  CATTGGGACAAAAAGATATGACTCCAATTCTGGTGGTGAGAGAGAAATTCAGCGAACAAT
  GTTGGAACTGCTGAACCAGTTGGATGGATTTGATTCTAGGGGAGATGTGAAAGTTATCAT
  GGCCACAAACCGAATAGAAACTTTGGATCCAGCACTTATCAGACCAGGCCGCATTGACAG
  GAAGATTGAGTTCCCCCTGCCTGATGAAAAGACGAAGAAGCGCATCTTTCAGATTCACAC
  AAGCAGGATGACGCTGGCTGATGATGTAACCCTGGACGACCTGATCATGGCTAAAGATGA
  CCTCTCTGGTGCTGACATCAAGGCAATCTGTACAGAAGCTGGTCTGATGGCCTTAAGAGA
  ACGTAGAATGAAAGTAACAAATGAAGACTTCAAAAAATCTAAAGAAAATGTTCTTTATAA
  GAAACAGGAAGGCACCCCTGAGGGGCTGTATCTCTAATGAACCATGGCTGTCATCAGGAA
  AATGGTTGGGAGATTTCTCAATCCCTGAAAGGGATGAGGTTGGGGGAGTTGCCCAGAGGA
  ATCCCTGTTCCCACTGATTTTTATTAGCAAAACATCCTGTGTCTTTTGGAGTACGATGTG
  TAAGTGCCCATTGGGTGGCCTGTTGGTCACTGTGCAGCAGTCTGCTTCCCAATAAAGCGT
  GCTCTTTCACAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002802
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002802.2, NP_002793.2
• RefSeq Size: 1586 bp
• RefSeq ORF: 1323 bp
• Locus ID: 5700
• Cytogenetics: 14q32.11
• Domains: AAA, AAA
• Protein Pathways: Proteasome
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. This subunit also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome. Finally, this subunit interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (a).