DHFR (NM_000791) Human Untagged Clone

CAT#: SC321334

DHFR (untagged)-Human dihydrofolate reductase (DHFR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHFR
• Synonyms: DHFRP1; DYR
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000791.3 CTTGACCGCGCGTTCTGCTGTAACGAGCGGGCTCGGAGGTCCTCCCGCTGCTGTCATGGT
  TGGTTCGCTAAACTGCATCGTCGCTGTGTCCCAGAACATGGGCATCGGCAAGAACGGGGA
  CCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATTTCCAGAGAATGACCACAACCTC
  TTCAGTAGAAGGTAAACAGAATCTGGTGATTATGGGTAAGAAGACCTGGTTCTCCATTCC
  TGAGAAGAATCGACCTTTAAAGGGTAGAATTAATTTAGTTCTCAGCAGAGAACTCAAGGA
  ACCTCCACAAGGAGCTCATTTTCTTTCCAGAAGTCTAGATGATGCCTTAAAACTTACTGA
  ACAACCAGAATTAGCAAATAAAGTAGACATGGTCTGGATAGTTGGTGGCAGTTCTGTTTA
  TAAGGAAGCCATGAATCACCCAGGCCATCTTAAACTATTTGTGACAAGGATCATGCAAGA
  CTTTGAAAGTGACACGTTTTTTCCAGAAATTGATTTGGAGAAATATAAACTTCTGCCAGA
  ATACCCAGGTGTTCTCTCTGATGTCCAGGAGGAGAAAGGCATTAAGTACAAATTTGAAGT
  ATATGAGAAGAATGATTAATATGAAGGTGTTTTCTAGTTTAAGTTGTTCCCCCTCCCTCT
  GAAAAAAGTATGTATTTTTACATTAGAAAAGGTTTTTTGTTGACTTTAGATCTATAATTA
  TTTCTAAGCAACTAGTTTTTATTCCCCACTACTCTTGTCTCTATCAGATACCATTTATGA
  GACATTCTTGCTATAACTAAGTGCTTCTCCAAGACCCCAACTGAGTCCCCAGCACCTGCT
  ACAGTGAGCTGCCATTCCACACCCATCACATGTGGCACTCTTGCCAGTCCTTGACATTGT
  CGGGCTTTTCACATGTTGGTAATATTTATTAAAGATGAAGATCCACATACCCTTCAACTG
  AGCAGTTTCACTAGTGGAAATACCAAAAGCTTCCTACGTGTATATCCAGAGGTTTGTAGA
  TAAATGTTGCCACCTTGTTTGTAACAGTGAAAAATTGAAAACAACCTGGAAGTCCAGTGA
  TGGGAAAATGAGTATGTTTCTGTCTTAGATTGGGGAACCCAAAGCAGATTGCAAGACTGA
  AATTTCAGTGAAAGCAGTGTATTTGCTAGGTCATACCAGAAATCATCAATTGAGGTACGG
  AGAAACTGAACTGAGAAGGTAAGAAAAGCAATTTAAAGTCAGCGAGCAGGTTCTCATTGA
  TAACAAGCTCCATACTGCTGAGATACAGGGAAATGGAGGGGGGAAAGCTGGAGTATTGAT
  CCCGCCCCCCTCCTTGGTTGTCAGCTCCCTGTCCTGTGTGTGGGCGGAACATAGTCCAGC
  TGCTCTATAGCAAGTCTCAGGTGTTTGCAGTAAGAAGCTGCTGGCATGCACGGGAACAGT
  GAATGCCAAACACTTAAAGCAATTCGATGTTTAAGTATGTAAGTTCTTTTTTTTTTAGAC
  AGCGTTTCGCTCTTGTTGCCCAGGCTGGCATGCAGTGGTGTGACCTCGGCTTACTGCAAC
  CTCCGCCTTCCCAGATTCAAGCGATTCTCCTGCCTCAGGCTCCCAAGTAGCTAGGACCAG
  GTGCGCGCCACCACGCCCGGCTAATTTTTGTATTTTGTATTTTTAGTAGAGATGGGGTTT
  CACCATGTTGGTCAGGCTGGTCTCGAACTCGTGACCGCAAGCGATTCACCCACCTCAGCC
  TCCCAAAGTGCTGGGATTACCGGCTTGAGCCACCACACCCGGCACATCTTCATTCTTTTT
  ATGTAGTAAAAAGTATAAGGCCACACATGGTTTATTTGAAGTATTTTATAATTTAAAAAA
  ATACAGAAGCAGGAAAACCAATTATAAGTTCAAGTGAGGGATGATGGTTGCTTGAACCAA
  AGGGTTGCATGTAGTAAGAAATTGTGATTTAAGATATATTTTAAAGTTATAAGTAGCAGG
  ATATTCTGATGGAGTTTGACTTTGGTTTTGGGCCCAGGGAGTTTCAGATGCCTTTGAGAA
  ATGAATGAAGTAGAGAGAAAATAAAAGAAAAACCAGCCAGGCACAGTGGCTCACGCCTGT
  AATCCCAGCGCTTTGGGAGGCTAAGGCAGGCAGATCACTTGAGACCAGCTTGGGCAACAT
  GGCAAAGCCCCATCTCTACAAAAAACACAAAAATTAGCTGGGCATTGTGGCGCACACCTG
  TATTCCCATCTAGTCAGGAAGCTGAGATGGAAGAATTAATTGAGCCCACGAGTTCAAGGC
  TGCAGTGAGTCGTGATTGTGCCACTGCACTCCAGCCGGGGTGACAGAAGAGACCTTGTCT
  CGAAAAGGAATCTGAAAACAATGGAACCATGCCTTCATAATTCTAGAAAGTTATTTTCAA
  CTGATAAATCTATATTCACCCAAATAATCAAGGGTGAAGGTAAAATAATACATTTTTAGA
  CAAGCAAAGACTCAGGGGTTACCTCCATGTGCCCTTTTTAGGGAAGCTGTTGGAGAAAAT
  ACTCCAGCAAAATGAAGGAGTACACAAACCAGAGAATGACATGAATCCAGCAAATAGGAT
  CCAACACAGGCAATATTCCAGCTATGGAGCTAGCTTTAAAAAGGAACAGTAAAAATATTA
  ATCGGTTAGCTGGGTGGAATGGCCCATGCCTGTAGTCCCAGCTACTCAGGAGGCTCAGCA
  GCAGGACGACTTGAGCCCAAGAGTTCCAGACCAGCCTGGCCACCTTAGTGAGATCCCTTC
  TCTTAAAAATAATAACTTATTGCCAGATTTGGGGCATTTGGAAAGAAGTTCATTGAAGAT
  AAAGCAAAAGTAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000791
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000791.3, NP_000782.1
• RefSeq Size: 3932 bp
• RefSeq ORF: 564 bp
• Locus ID: 1719
• Cytogenetics: 5q14.1
• Domains: DiHfolate_red
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Folate biosynthesis, Metabolic pathways, One carbon pool by folate
• Gene Summary: 'Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).