NANS (NM_018946) Human Untagged Clone

CAT#: SC321367

NANS (untagged)-Human N-acetylneuraminic acid synthase (NANS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NANS
• Synonyms: HEL-S-100; SAS; SEMDCG; SEMDG
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018946.2 CACAGAACAGAGTAGAGGCGGCGGCGGCGGCGGCCGGACCCAGACTGGTAGTGAGGCGTT
  GGACCCCGAGCCGCTGCAATGCCGCTGGAGCTGGAGCTGTGTCCCGGGCGCTGGGTGGGC
  GGGCAACACCCGTGCTTCATCATTGCCGAGATCGGCCAGAACCACCAGGGCGACCTGGAT
  GTAGCCAAGCGCATGATCCGCATGGCCAAGGAGTGTGGGGCTGATTGTGCCAAGTTCCAG
  AAGAGTGAGCTAGAATTCAAGTTTAATCGGAAAGCCTTGGAGAGGCCATACACCTCGAAG
  CATTCCTGGGGGAAGACGTACGGGGAGCACAAACGACATCTGGAGTTCAGCCATGACCAG
  TACAGGGAGCTGCAGAGGTACGCCGAGGAGGTTGGGATCTTCTTCACTGCCTCTGGCATG
  GATGAGATGGCAGTTGAATTCCTGCATGAACTGAATGTTCCATTTTTCAAAGTTGGATCT
  GGAGACACTAATAATTTTCCTTATCTGGAAAAGACAGCCAAAAAAGGTCGCCCAATGGTG
  ATCTCCAGTGGGATGCAGTCAATGGACACCATGAAGCAAGTTTATCAGATCGTGAAGCCC
  CTCAACCCCAACTTCTGCTTCTTGCAGTGTACCAGCGCATACCCGCTCCAGCCTGAGGAC
  GTCAACCTGCGGGTCATCTCGGAATATCAGAAGCTCTTTCCTGACATTCCCATAGGGTAT
  TCTGGGCATGAAACAGGCATAGCGATATCTGTGGCCGCAGTGGCTCTGGGGGCCAAGGTG
  TTGGAACGTCACATAACTTTGGACAAGACCTGGAAGGGGAGTGACCACTCGGCCTCGCTG
  GAGCCTGGAGAACTGGCCGAGCTGGTGCGGTCAGTGCGTCTTGTGGAGCGTGCCCTGGGC
  TCCCCAACCAAGCAGCTGCTGCCCTGTGAGATGGCCTGCAATGAGAAGCTGGGCAAGTCT
  GTGGTGGCCAAAGTGAAAATTCCGGAAGGCACCATTCTAACAATGGACATGCTCACCGTG
  AAGGTGGGTGAGCCCAAAGGCTATCCTCCTGAAGACATCTTTAATCTAGTGGGCAAGAAG
  GTCCTGGTCACTGTTGAAGAGGATGACACCATCATGGAAGAATTGGTAGATAATCATGGC
  AAAAAAATCAAGTCTTAAAAATAAAGTGCCATTCTCTGAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018946.2 unedited
  GACGATTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGA
  GGAGATCTGCCGCCGCGATCGCGGCGCGCCAGATCTCAAGCTTAACTAGTTAGCGGACCG
  ACGCGTTAAGCGGCCGCGAATTCAGATCCACAAGTTTGTACAAAAAAGCAGGCTTGTAAA
  ACGACGGCCAGTAACTATAACGGTCCTAAGGTAGCGAGGCCTGGGTGGCGAATTCGGCAC
  GAGGCACAGAACAGAGTAGAGGCGGCGGCGGCGGCGGCCGGACCCAGACTGGTAGTGAGG
  CGTTGGACCCCGAGCCGCTGCAATGCCGCTTGATCTGGAGCTGTGTCCCGGGCGCTGGGT
  GGGCGGGCAACACCCGTGCTTCATCATTGCCGAGATCGGCCAGAACCACCAGGGCGACCT
  GGATGTAGCCAAGCGCATGATCCGCATGGCCAAGGAGTGTGGGGCTGATTGTGCCAAGTT
  CCAGAAGAGTGAGCTAGAATTCAAGTTTAATCGGAAAGCCTTGGAGAGGCCATACACCTC
  GAAGCATTCCTGGGGGAAGACGTACGGGGAGCACAAACGACATCTGGAGTTCAGCCATGA
  CCAGTACAGGGAGCTGCAGAGGTACGCCGAGGAGGTTGGGATCTTCTTCACTGCCTCTGG
  CATGGATGAGATGGCAGTTGAATTCCTGCATGAACTGAATGTTCCATTTTTCAAAGTTGG
  ATCTGGAGACACTAATAATTTTCCTTATCTGGAAAAGACAGCCAAAAAAGGTCGCCCAAT
  GGTGATCTCCAGTGGGATGCAGTCAATGGACACCATGAAGCAAGTTTATCAGATCGTGAA
  GCCCTTCACCCCCAACTTCTGCTCCTTGCAGTGTATCAGCGCATAACCCGCCTCCAGCCT
  CGAGGACGTTCATCTGCGGTCATCTTCGGAATATTCAAAAGCCTCTTTTACTGACCTTCC
  CCTAAGGTTATTTCT
• Restriction Sites: Please inquire
• ACCN: NM_018946
• ORF Size: 1080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_018946.2, NP_061819.2
• RefSeq Size: 1170
• RefSeq ORF: 1080
• Locus ID: 54187
• Domains: NeuB, Antifreeze
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways
• Gene Summary: This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]