DAB2 (NM_001343) Human Untagged Clone

CAT#: SC321375

DAB2 (untagged)-Human disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila) (DAB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAB2
• Synonyms: DOC-2; DOC2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001343.1 CGCTTCACGGAGGCAATAGCTAGCCGGTGTCTGTGGGAGGTTATGTTTATTTGAGAGTTC
  TCCATCGGGATCGCCTGGTGTCACCAAGTGTCCACTGGTACTGAGGTTTGCTGCCTGCCT
  TCTTGCCATGTCTAACGAAGTAGAAACAAGTGCAACCAATGGTCAGCCCGACCAACAGGC
  CGCACCAAAAGCACCCTCAAAGAAGGAAAAAAAGAAAGGCCCTGAAAAGACAGATGAATA
  TCTCTTAGCAAGGTTCAAAGGCGATGGTGTAAAATATAAGGCCAAGCTGATTGGCATTGA
  TGATGTGCCAGATGCAAGAGGGGATAAAATGAGCCAAGACTCTATGATGAAACTAAAGGG
  AATGGCGGCAGCTGGTCGGTCTCAGGGACAACACAAACAAAGGATCTGGGTCAACATTTC
  CCTTTCTGGGATAAAAATAATTGATGAGAAAACTGGGGTAATAGAGCATGAACATCCAGT
  AAATAAGATTTCTTTCATTGCCCGTGATGTGACAGACAACCGGGCATTTGGTTACGTGTG
  TGGAGGAGAAGGCCAGCATCAGTTTTTTGCCATAAAAACCGGGCAACAGGCTGAACCATT
  AGTTGTTGATCTTAAAGACCTTTTTCAAGTTATCTATAATGTAAAGAAAAAGGAAGAAGA
  AAAGAAAAAGATAGAGGAAGCCAGCAAAGCAGTTGAGAATGGGAGTGAGGCCCTAATGAT
  TCTAGATGACCAAACTAACAAACTGAAATCGGGTGTTGACCAGATGGATTTGTTTGGGGA
  CATGTCTACACCTCCTGACCTAAATAGTCCAACAGAAAGCAAAGATATCCTGTTAGTGGA
  TCTAAACTCTGAAATCGACACCAATCAGAATTCTTTAAGAGAAAATCCATTCTTAACAAA
  CGGCATCACCTCCTGTTCTCTTCCTCGACCAACGCCTCAGGCATCCTTCTTGCCTGAAAA
  TGCCTTTTCTGCCAATCTCAACTTCTTTCCCACCCCTAATCCTGATCCTTTCCGTGACGA
  TCCTTTCACACAGCCAGACCAATCGACACCTTCTTCGTTTGATTCTCTCAAATCTCCAGA
  TCAGAAGAAAGAGAATTCGAGTAGCTCGTCTACTCCGCTGAGTAATGGGCCCCTGAATGG
  TGATGTTGACTACTTTGGTCAGCAATTTGACCAGATCTCTAACCGGACTGGCAAACAGGA
  AGCTCAGGCAGGCCCATGGCCCTTTTCAAGTTCGCAAACCCAGCCAGCAGTGAGAACTCA
  AAATGGGGTATCTGAAAGAGAACAGAACGGCTTCTCTGTCAAATCCTCCCCGAACCCTTT
  TGTGGGAAGCCCTCCCAAAGGACTGTCCATACAGAATGGCGTAAAGCAGGACTTGGAAAG
  CTCTGTCCAGTCCTCACCACATGACTCCATAGCCATTATCCCACCTCCACAAAGTACCAA
  ACCAGGAAGAGGCAGAAGGACTGCTAAGTCTTCAGCCAATGACTTGCTTGCATCAGACAT
  CTTTGCTCCTCCCGTCTCAGAACCTTCAGGCCAGGCGTCACCCACAGGACAACCTACAGC
  CCTGCAGCCCAACCCTCTGGATCTCTTCAAAACAAGTGCTCCTGCCCCAGTGGGGCCCCT
  GGTGGGTCTAGGTGGTGTAACTGTCACACTCCCTCAGGCAGGACCATGGAACACAGCATC
  TTTGGTCTTCAATCAGTCCCCTTCAATGGCTCCGGGAGCCATGATGGGTGGTCAACCTTC
  AGGTTTTAGTCAGCCCGTCATTTTTGGTACAAGTCCAGCTGTTTCAGGTTGGAACCAGCC
  TTCACCCTTTGCAGCCTCAACTCCCCCTCCAGTGCCTGTTGTCTGGGGCCCTTCTGCATC
  TGTGGCACCCAATGCTTGGTCAACAACAAGCCCTTTGGGGAATCCTTTTCAGAGCAATAT
  TTTTCCAGCTCCTGCTGTGTCCACTCAGCCCCCATCCATGCACTCCTCTCTCCTGGTCAC
  TCCTCCTCAGCCACCTCCCAGAGCTGGCCCTCCCAAGGACATCTCCAGTGATGCCTTCAC
  TGCCTTAGACCCACTTGGGGATAAAGAGATCAAGGATGTGAAAGAAATGTTTAAGGATTT
  CCAACTGCGGCAGCCACCTGCTGTGCCCGCGCGGAAGGGAGAGCAGACTTCTTCTGGGAC
  TTTGAGTGCCTTTGCCAGTTATTTCAACAGCAAGGTTGGCATTCCTCAGGAGAATGCAGA
  CCATGATGACTTTGATGCTAATCAACTATTGAACAAGATCAATGAACCACCAAAGCCAGC
  TCCCAGACAAGTTTCCCTGCCAGTTACCAAATCTACTGACAATGCATTTGAGAACCCTTT
  CTTTAAAGATTCTTTTGGTTCATCACAAGCCTCTGTGGCTTCTTCTCAACCTGTATCTTC
  TGAGATGTATAGGGATCCATTTGGAAATCCTTTTGCCTAAATTCTGAACTTGGTCTGCAG
  ACCATCCAGAGGAATAAAAAGGTTGGCCTTAGTAGTCAAAAACAAAGCTGATAGCCAGAC
  ACGTTCTGATTTCTGCCCTTGTTCCAGCTTTGACGTATTATCTGTTGCCTTATTTCTCAT
  TGCCTCTTCTACTTGTAAAATGCTTTTCACTTTCTGTCTAGGTTAAAGCTAAACTGAATC
  TATGGCTTTAAATAAATTAAGATCCTAAACTCTCTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001343
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001343.1, NP_001334.1
• RefSeq Size: 3268 bp
• RefSeq ORF: 2313 bp
• Locus ID: 1601
• Cytogenetics: 5p13.1
• Domains: PID
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: 'This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).