NCF1 (NM_000265) Human Untagged Clone

CAT#: SC321404

NCF1 (untagged)-Human neutrophil cytosolic factor 1 (NCF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCF1
• Synonyms: CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000265.4 CGACTTCCTCTTTCCAGTGCATTTAAGGCGCAGCCTGGAAGTGCCAGGGAGCACTGGAGG
  CCACCCAGTCATGGGGGACACCTTCATCCGTCACATCGCCCTGCTGGGCTTTGAGAAGCG
  CTTCGTACCCAGCCAGCACTATGTGTACATGTTCCTGGTGAAATGGCAGGACCTGTCGGA
  GAAGGTGGTCTACCGGCGCTTCACCGAGATCTACGAGTTCCATAAAACCTTAAAAGAAAT
  GTTCCCTATTGAGGCAGGGGCGATCAATCCAGAGAACAGGATCATCCCCCACCTCCCAGC
  TCCCAAGTGGTTTGACGGGCAGCGGGCCGCCGAGAACCGCCAGGGCACACTTACCGAGTA
  CTGCAGCACGCTCATGAGCCTGCCCACCAAGATCTCCCGCTGTCCCCACCTCCTCGACTT
  CTTCAAGGTGCGCCCTGATGACCTCAAGCTCCCCACGGACAACCAGACAAAAAAGCCAGA
  GACATACTTGATGCCCAAAGATGGCAAGAGTACCGCGACAGACATCACCGGCCCCATCAT
  CCTGCAGACGTACCGCGCCATTGCCAACTACGAGAAGACCTCGGGCTCCGAGATGGCTCT
  GTCCACGGGGGACGTGGTGGAGGTCGTAGAGAAGAGCGAGAGCGGTTGGTGGTTCTGTCA
  GATGAAAGCAAAGCGAGGCTGGATCCCAGCGTCCTTCCTCGAGCCCCTGGACAGTCCTGA
  CGAGACGGAAGACCCTGAGCCCAACTATGCAGGTGAGCCATACGTCGCCATCAAGGCCTA
  CACTGCTGTGGAGGGGGACGAGGTGTCCCTGCTCGAGGGTGAAGCTGTTGAGGTCATTCA
  CAAGCTCCTGGACGGCTGGTGGGTCATCAGGAAAGACGACGTCACAGGCTACTTCCCGTC
  CATGTACCTGCAAAAGTCAGGGCAAGACGTGTCCCAGGCCCAACGCCAGATCAAGCGGGG
  GGCGCCGCCCCGCAGGTCGTCCATCCGCAACGCGCACAGCATCCACCAGCGGTCGCGGAA
  GCGCCTCAGCCAGGACGCCTATCGCCGCAACAGCGTCCGTTTTCTGCAGCAGCGACGCCG
  CCAGGCGCGGCCGGGACCGCAGAGCCCCGGGAGCCCGCTCGAGGAGGAGCGGCAGACGCA
  GCGCTCTAAACCGCAGCCGGCGGTGCCCCCGCGGCCGAGCGCCGACCTCATCCTGAACCG
  CTGCAGCGAGAGCACCAAGCGGAAGCTGGCGTCTGCCGTCTGAGGCTGGAGCGCAGTCCC
  CAGCTAGCGTCTCGGCCCTTGCCGCCCCGTGCCTGTACATACGTGTTCTATAGAGCCTGG
  CGTCTGGACGCCGAGGGCAGCCCCGACCCCTGTCCAGCGCGGCTCCCGCCACCCTCAATA
  AATGTTGCTTGGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000265
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000265.4, NP_000256.3
• RefSeq Size: 1409 bp
• RefSeq ORF: 1173 bp
• Locus ID: 653361
• Cytogenetics: 7q11.23
• Domains: SH3, PX
• Protein Pathways: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]