Carbonic Anhydrase III (CA3) (NM_005181) Human Untagged Clone

CAT#: SC321507

CA3 (untagged)-Human carbonic anhydrase III, muscle specific (CA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CA3
• Synonyms: CAIII; Car3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005181.2 GAGAAAGCAGGAGCCGTCCAGCACGGAGGAAGGCGACCATGGCCAAGGAGTGGGGCTACG
  CCAGTCACAACGGTCCTGACCACTGGCATGAACTTTTCCCAAATGCCAAGGGGGAAAACC
  AGTCGCCCATTGAGCTGCATACTAAAGACATCAGGCATGACCCTTCTCTGCAGCCATGGT
  CTGTGTCTTATGATGGTGGCTCTGCCAAGACCATCCTGAATAATGGGAAGACCTGCCGAG
  TTGTATTTGATGATACTTATGATAGGTCAATGCTGAGAGGGGGTCCTCTCCCTGGACCCT
  ACCGACTTCGCCAGTTTCATCTTCACTGGGGCTCTTCGGATGATCATGGCTCTGAGCACA
  CCGTGGATGGAGTCAAGTATGCAGCGGAGCTTCATTTGGTTCACTGGAACCCGAAGTATA
  ACACTTTTAAAGAAGCCCTGAAGCAGCGCGATGGGATCGCTGTGATTGGCATTTTTCTGA
  AGATAGGACATGAGAATGGCGAGTTCCAGATTTTCCTTGATGCATTGGACAAGATTAAGA
  CAAAGGGCAAGGAGGCGCCCTTCACAAAGTTTGACCCATCCTGCCTGTTCCCGGCATGCC
  GGGACTACTGGACCTACCAGGGCTCATTCACCACGCCGCCCTGCGAGGAATGCATTGTGT
  GGCTGCTGCTGAAGGAGCCCATGACCGTGAGCTCTGACCAGATGGCCAAGCTGCGGAGCC
  TCCTCTCCAGTGCTGAGAACGAGCCCCCAGTGCCTCTTGTGAGCAACTGGCGACCTCCAC
  AGCCTATCAATAACAGGGTGGTGAGAGCTTCCTTCAAATGAGGCTGCTGGATCTTGCCCT
  CTTCAGGAAAGGAAACCTACCATTGGAGAGCTTGGTTCCTTGCCTCCTTCTGGTGCTCTT
  ACTCCAAGTCTATTTCATTTTTCCACACTGAGCAATGAATGTGAGAGATGTGGTCACCAA
  GATCTAAGTTACTTGTTGAAAGAAAGTTACTTTCGACAAGATCTAATATGAAAGCATAGA
  TTTCACATTTGATCTCTGTAATAATCATCTTTCCTATAAAAGTAGCATTTTTGGTAAAGT
  TTCAAAGAAGAAGAAACAGAGATGGAAGAGTAAAGATATTTTTAAAATGGCTAGCTATTG
  GGCACCAGTTTTTCTGTTATCTAAAATTTCACACAACTTCATTGTTTTTATTTTTATATT
  ATGAGTTGTCCATCTTAAAGAAATATGAGTAATTCTACATGTAGTAGAGGTGTATGAAGA
  TCATATAACAATTAAACATAAGCCAGAAATTAAAATGACTATAGACAGCAAGAATTGAGC
  TAATAATATGTTTTAACTCTTAACACCAGCAAGAAGTCAGTCATTTATTGAAGTTTTAGC
  TACTAAGATTACTTGGTTTTGATTACCAGTGAAAAGAAAACACAATACAATCAGGAGTTT
  TCAAATTTTTGATTCAGTATTTGAATTTCTTCTTCATAAATGTAGTTGAATTTATCCTAG
  TATTTTTCTTTACCTGAAGTAGGGCCATTTATTTTTAATTTCACTACATTTTTCTTTGCA
  TGATTATTAAAATAAAAACTGCCTCTGTTGTGTTTCTCACTGGAGGCTGGAATGAATGAT
  CACTAGAACACAAAAGAGTGAATGATGACACTTGAAGTCAAAGCAGTTGTACTGATCACC
  AGAACCAATAAAGACATAAATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005181
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005181.2, NP_005172.1
• RefSeq Size: 2357 bp
• RefSeq ORF: 783 bp
• Locus ID: 761
• Cytogenetics: 8q21.2
• Domains: carb_anhydrase
• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism
• Gene Summary: 'Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]'