RTKN (NM_033046) Human Untagged Clone

CAT#: SC321545

RTKN (untagged)-Human rhotekin (RTKN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RTKN
• Synonyms: B5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033046.2 GGAAAATTTGTCACAGCAGCCAGAGGGGTTTAACAGGAGTGCAGAGGGATAAGGGCAGCT
  TCTGCCCTCTGCCCAAGAGCTGGCCACCTCTTTAAAGACTGAGGGAACAGTGGGAGGAGG
  AACTGTGGGACAGTGTGGTACCTATCTGTCCCCCCTCTGGAGGGGTTGACAAGGGAAAGG
  GCACCGGGGGGCACAGAGATGCAGGACAGATTGCACATCCTGGAGGACCTGAATATGCTC
  TACATTCGGCAGATGGCACTCAGCCTGGAGGACACGGAGTTGCAGAGGAAGCTAGACCAT
  GAGATCCGGATGAGGGAAGGGGCCTGTAAGCTGCTGGCAGCCTGCTCCCAGCGAGAGCAG
  GCTCTGGAGGCCACCAAGAGCCTGCTAGTGTGCAACAGCCGCATCCTCAGCTACATGGGC
  GAGCTGCAGCGGCGCAAGGAGGCGCAGGTGCTGGGGAAGACAAGCCGGCGGCCTTCTGAC
  AGTGGCCCGCCCGCTGAGCGCTCCCCCTGCCGCGGCCGGGTCTGCATCTCTGACCTCCGG
  ATTCCACTCATGTGGAAGGACACAGAATATTTCAAGAACAAAGGTGACTTGCACCGCTGG
  GCTGTGTTCCTGCTGCTGCAGCTGGGGGAACACATCCAGGACACAGAGATGATCCTAGTG
  GACAGGACCCTCACAGACATCTCCTTTCAGAGCAATGTGCTCTTCGCTGAGGCGGGGCCA
  GACTTTGAACTGCGGTTAGAGCTGTATGGGGCCTGTGTGGAAGAAGAGGGGGCCCTGACT
  GGCGGCCCCAAGAGGCTTGCCACCAAACTCAGCAGCTCCCTGGGCCGCTCCTCAGGGAGG
  CGTGTCCGGGCATCGCTGGACAGTGCTGGGGGTTCAGGGAGCAGTCCCATCTTGCTCCCC
  ACCCCAGTTGTTGGTGGTCCTCGTTACCACCTCTTGGCTCACACCACACTCACCCTGGCA
  GCAGTGCAAGATGGATTCCGCACACATGACCTCACCCTTGCCAGTCATGAGGAGAACCCT
  GCCTGGCTGCCCCTTTATGGTAGCGTGTGTTGCCGTCTGGCAGCTCAGCCTCTCTGCATG
  ACTCAGCCCACTGCAAGTGGTACCCTCAGGGTGCAGCAAGCTGGGGAGATGCAGAACTGG
  GCACAAGTGCATGGAGTTCTGAAAGGCACAAACCTCTTCTGTTACCGGCAACCTGAGGAT
  GCAGACACTGGGGAAGAGCCGCTGCTTACTATTGCTGTCAACAAGGAGACTCGAGTCCGG
  GCAGGGGAGCTGGACCAGGCTCTAGGACGGCCCTTCACCCTAAGCATCAGTAACCAGTAT
  GGGGATGATGAGGTGACACACACCCTTCAGACAGAAAGTCGGGAAGCACTGCAGAGCTGG
  ATGGAGGCTCTGTGGCAGCTTTTCTTTGACATGAGCCAATGGAAGCAGTGCTGTGATGAA
  ATCATGAAAATTGAAACTCCTGCTCCCCGGAAACCACCCCAAGCACTGGCAAAGCAGGGG
  TCCTTGTACCATGAGATGGCTATTGAGCCGCTGGATGACATCGCAGCGGTGACAGACATC
  CTGACCCAGCGGGAGGGCGCAAGGCTGGAGACACCCCCACCCTGGCTGGCAATGTTTACA
  GACCAGCCTGCCCTGCCTAACCCCTGCTCGCCTGCCTCAGTGGCCCCAGCCCCAGACTGG
  ACCCACCCCCTGCCCTGGGGGAGACCCCGAACCTTTTCCCTGGATGCTGTCCCCCCAGAC
  CACTCCCCTAGGGCTCGCTCGGTTGCCCCCCTCCCACCTCAGCGATCCCCACGGACCAGA
  GGCCTCTGCAGCAAAGGCCAACCTCGCACTTGGCTCCAGTCACCAGTGTGAGAGAGAAAG
  GTGCTGGCATAGGATCTGCCCAGAAGAGAAAATGACCCATGCGCAGTTGGGCTCTGGATA
  CGGCGCTGTCTATAGCAAGTTGGCCAGTCTGGCCTCCTGTTCCTCTGCTGGACCTGGGGT
  AGGCTGCAGGGGTGGGCAGAAGCCCCTCTTAAATTGTGGTTGCCATGGTACCGAGGGACT
  CATTCCTGGGGCTCGCTGGGACCTCCCTAAACCCTTCCTGGAAGAAAACTGGAACCAACT
  CTGCCCTACCTCCCTGCACTAACCAGCTTTGAGGATGGCACTGAAGAACCCTTGGAGCAA
  ACATACCTCCCTTGTGACTCCCACATCAACCATTAAAGTTATTTAACAGCAAAAAAAAAA
  AAAAA
• Restriction Sites: Please inquire
• ACCN: NM_033046
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033046.2, NP_149035.1
• RefSeq Size: 2638 bp
• RefSeq ORF: 1653 bp
• Locus ID: 6242
• Cytogenetics: 2p13.1
• Domains: PH
• Gene Summary: 'This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. The encoded protein (isoform b) is shorter and has a distinct N-terminus, compared to isoform a.