AMPK gamma 1 (PRKAG1) (NM_002733) Human Untagged Clone

CAT#: SC321552

PRKAG1 (untagged)-Human protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKAG1
• Synonyms: AMPKG
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002733.3 GCGAGGAAACCAAGCCGGGCGCCTCTTGCAATGGAGACGGTCATTTCTTCAGATAGCTCC
  CCAGCTGTGGAAAATGAGCATCCTCAAGAGACCCCAGAATCCAACAATAGCGTGTATACT
  TCCTTCATGAAGTCTCATCGCTGCTATGACCTGATTCCCACAAGCTCCAAATTGGTTGTA
  TTTGATACGTCCCTGCAGGTGAAGAAAGCTTTTTTTGCTTTGGTGACTAACGGTGTACGA
  GCTGCCCCTTTATGGGATAGTAAGAAGCAAAGTTTTGTGGGCATGCTGACCATCACTGAT
  TTCATCAATATCCTGCACCGCTACTATAAATCAGCCTTGGTACAGATCTATGAGCTAGAA
  GAACACAAGATAGAAACTTGGAGAGAGGTGTATCTCCAGGACTCCTTTAAACCGCTTGTC
  TGCATTTCTCCTAATGCCAGCTTGTTTGATGCTGTCTCTTCATTAATTCGGAACAAGATC
  CACAGGCTGCCAGTTATTGACCCAGAATCAGGCAATACTTTGTACATCCTCACCCACAAG
  CGCATTCTGAAGTTCCTCAAATTGTTTATCACTGAGTTCCCCAAGCCAGAGTTCATGTCC
  AAGTCTCTGGAAGAGCTACAGATTGGCACCTATGCCAATATTGCTATGGTTCGCACTACC
  ACCCCCGTCTATGTGGCTCTGGGGATTTTTGTACAGCATCGAGTCTCAGCCCTGCCAGTG
  GTGGATGAGAAGGGGCGTGTGGTGGACATCTACTCCAAGTTTGATGTTATCAATCTGGCA
  GCAGAAAAGACCTACAACAACCTAGATGTATCTGTGACTAAAGCCTTGCAACATCGATCA
  CATTACTTTGAGGGTGTTCTCAAGTGCTACCTGCATGAGACTCTGGAGACCATCATCAAC
  AGGCTAGTGGAAGCAGAGGTTCACCGACTTGTAGTGGTGGATGAAAATGATGTGGTCAAG
  GGAATTGTATCACTGTCTGACATCCTGCAGGCCCTGGTGCTCACAGGTGGAGAGAAGAAG
  CCCTGAGCTGGGGGAAGGGGTCATGCAGCACCAGGGGATATGCCCAACTCACTGCCTGCT
  GGAAGCTCTGTGGGAATCAGATGAAACTTGAGGGAATTGTGACTCTGTTCCCTGTTCAGG
  GTCCCCTGCCCTTCTATCTGGGAGCTAGGGAAGGTATGGGGGAGGAAAGAGAATGGATTT
  ATAGCTACCCTTACCCTCACACATACACTTGAAAAAACTTTCAGCCTAGCCAGTTCTAGC
  CCCTGTCCTCTTAGATATATCCCCCTTTCTGGGTGAACTATAGGCTCTGTGCCTCTCAGA
  CAAATTCTGATCTCTAAGAGATCCCCAGACCTCACTTGCCTCTGCCTCCATCTTGGCCCT
  GATTCAACCCTAAGATAATAGCACAACAAAATTCTTCATAAAGATATTTTTATTCACCTG
  TTCCGTGCTATATGGAGGAGGCCAAGTCCATTTAGTGACATTTCTTCCCATAATGTGAGT
  GGGGAGGATTGTGGGGAGGAGGGGCTTTGGGTTCCTGTGTTTGTGCATATGAAGGGAGAT
  GGGGGTTAGGTGGAGGAGGAGAGCAGCGTGGTTAGCTAAGGTTATTGCTTTTTGTGGCAA
  ATCTAATTAAATGACAGGAATCTCAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002733
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002733.3, NP_002724.1
• RefSeq Size: 1707 bp
• RefSeq ORF: 996 bp
• Locus ID: 5571
• Cytogenetics: 12q13.12
• Domains: CBS
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway
• Gene Summary: 'The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes isoform 1.