PSME3 (NM_005789) Human Untagged Clone

CAT#: SC321554

PSME3 (untagged)-Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) (PSME3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSME3
• Synonyms: HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; REG-GAMMA
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005789.2 GGCGAAAGCCGGGAGGGCGAGCGAGAGAGCAAGCAGGCAGCAGGCTGCCGGCGGGCGGGC
  GGACGGCACAGAGGGAGGGAGCGAGCGAGCAGTGAGTAAGCCAGCAAGGGCGGTCGGGTC
  CCGAGGTCAGCCGAGATTTCTCAGGTCCCTCCGGCCCCCTCCCTGGAGTCCACAGCGCCT
  CCGGTGTCCAGAGGATCGGACACGGCCCGGCCCGGCCATGGCCTCGTTGCTGAAGGTGGA
  TCAGGAAGTGAAGCTCAAGGTTGATTCTTTCAGGGAGCGGATCACAAGTGAGGCAGAAGA
  CTTGGTGGCAAATTTTTTCCCAAAGAAGTTATTAGAACTTGATAGTTTTCTGAAGGAACC
  AATCTTAAACATCCATGACCTAACTCAGATCCACTCTGACATGAATCTCCCAGTCCCTGA
  CCCCATTCTTCTCACCAATAGCCATGATGGACTGGATGGTCCCACTTATAAGAAGCGAAG
  GTTGGATGAGTGTGAAGAAGCCTTCCAAGGAACCAAGGTGTTTGTGATGCCCAATGGGAT
  GCTGAAAAGCAACCAGCAGCTGGTGGACATTATTGAGAAAGTGAAACCTGAGATCCGGCT
  GTTGATTGAGAAATGTAACACGGTCAAAATGTGGGTACAGCTCCTGATTCCCAGGATAGA
  AGATGGAAACAACTTTGGGGTGTCCATTCAGGAGGAAACAGTTGCAGAGCTAAGAACTGT
  TGAGAGTGAAGCTGCATCTTATCTGGACCAGATTTCTAGATATTATATTACAAGAGCCAA
  ATTGGTTTCTAAAATAGCTAAATATCCCCATGTGGAGGACTATCGCCGCACCGTGACAGA
  GATTGATGAGAAAGAATATATCAGCCTTCGGCTCATCATATCAGAGCTGAGGAATCAATA
  TGTCACTCTACATGACATGATCCTGAAAAATATCGAGAAGATCAAACGGCCCCGGAGCAG
  CAATGCAGAGACTCTGTACTGAGGCCAGGGCCAGGGCCAGGGGACTCTGTGAGTCTGGCT
  CAAGACCGACATTGCCTTGGTTTGTTACATGACTATCGTGATGGGGAAACTGGCTGGAAA
  TAGTAATCACACCTCTCTGTTTTTAGTTAGAGTCTAATGAAACTCTCATCTAGTTCTGTG
  ATGTGTTTACCTCTTTTTTCAGGCCTCAGGAACTCTTCTATTTCCTTCCCTAATACCCCA
  CACCCAACCTGTCGTAATTTCTGGAGAACTCCAGGTTTGTGTGTGCAGGATGTTGGCACA
  AAAATACCTGTGTTTTCATTCTCCCCCTCTCTCCCTCCTGTGTCTTGCGCTTTATGTTTT
  CTTCCGTTTGATAATTAGTTGGTTAAAAGCTGAGGGAACCGGAAGGAAAGTGCTAGGTGT
  TTTTTAGGAACTAGGGTGGCGGGGGGACGAACTTCTCTTCCTCACATGAGGTTACTGTTT
  CTTTCCTCTGTGGGGCATTGGATCCTCCCACAGTTGCCCTGGTGATGACTTAGGGCTTCC
  CATCTGTGTACATCCCACTTTGAATCTTGATCGTGACAAGAAATACCTTAGGCCTTCAGT
  CAATTCCGAAGCTCCTTCAGTTGTTTTTATAATGGGCGTTTTCACATGCACATATGTGTA
  TGCATGTATACGCCCATACAGACATGCACACACAGACTCCTACTCCATTAGCTAACATAC
  CCTCCCTCTCCACAACCCCTGTCACATACCTTTCAGGAGGTGACAGTTGTCTTAGTTGTC
  ATCTACCCAGACAAACGTCCTGGGCCCGTCCTCCCTCCTGATACTGTAGCCTCTTGGTAC
  CCAGGGTGAGTTGGTGGAGAACAGAGAGATGAGAAGCAGAGGGCTTGGGGAAAGCCTGTT
  CCTCTCTGACTCAGCCCTTTTTGGCATTATTGCAAGAGCTTGACTCCTGGTTGCCTTTTC
  CCAGCCAGTTTTCAGTTGGGGTGAAGGTTTCTGCAAGTGTGAGGTCCAGATGCTGCTGCT
  CATGTTGGGCTTTCCTTTTGGGAACTATTTCTCTTTATTTATAGTGTCGGGCTTCCGGGG
  AAAGCAATCATTGGTGTGTATGTGTATGTGCATGCACACACGTGCATATACACATTTGTG
  TATGTGGAAATGTGCTGGGCAAGTCAAAACTATAGAAGAGTTGCCTCCTGTCTCTCGAAT
  CTTCCAGAGATATCACTTAATTGTTAACAGCTTTTGTGTTAATCCCCTTCAGCCCCTAGC
  TCTTTTATTCTACCACGGCTGGAGAGTTGATACCTGCAGTCAGCCTGCCAGTGACTCTTA
  GTGTCTGTTTCTGACTTATTTTTCCTGTCTCTGTCTTCCAACCCCCAATAATATTTCCAC
  CGGGGATGCATCATTTTTACTCCCAATATTCTGTAGAGAGGGAGTCAGGATGCTGTCTTC
  CCACGAATAGTACTCAGTAACAAACCAATTGCATTTTAGTTGGGCAGTGCTCCCACCCAC
  CCTCCAGATCCCTTCCAGCTAAAACCCTTCCCCCTTCCCTCCATGTGTTTCTCAGTTTCC
  CGTTTCGTTTGTTGGACTGTTCCACTGCCCCTCCTCCTCACCCTATCACCCATGGATCGT
  AATGTAAAATTCTTTTACCATGTCAAGAAATTATTAAAAATACAGGTACTTTGACCTCTT
  TCTAAAGCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005789.2 unedited
  GCGATTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGAGGGGCGAAAGCCGGG
  AGGGCGAGCGAGAGAGCAAGCAGGCAGCAGGCTGCCGGCGGGCGGGCGGACGGCACAGAG
  GGAGGGAGCGAGCGAGCAGTGAGTAAGCCAGCAAGGGCGGTCGGGTCCCGAGGTCAGCCG
  AGATTTCTCAGGTCCCTCCGGCCCCCTCCCTGGAGTCCACAGCGCCTCCGGTGTCCAGAG
  GATCGGACACGGCCCGGCCCGGCCATGGCCTCGTTGCTGAAGGTGGATCAGGAAGTGAAG
  CTCAAGGTTGATTCTTTCTTGTTTCGTATCACAAGTGAGGCAGAAGACTTGGTGGCAAAT
  TTTTTCCCAAAGAAGTTATTAGAACTTGATAGTTTTCTGAAGGAACCAATCTTAAACATC
  CATGACCTAACTCAGATCCACTCTGACATGAATCTCCCAGTCCCTGACCCCATTCTTCTC
  ACCAATAGCCATGATGGACTGGATGGTCCCACTTATAAGAAGCGAAGGTTGGATGAGTGT
  GAAGAAGCCTTCCAAGGAACCAAGGTGTTTGTGATGCCCAATGGGATGCTGAAAAGCAAC
  CAGCAGCTGGTGGACATTATTGAGAAAGTGAAACCTGAGATCCGGCTGTTGATTGAGAAA
  TGTAACACGGTCAAAATGTGGGTACAGCTCCTGATTCCCAGGATAGAAGATGGAAACAAC
  TTTGGGGTGTCCATTCAGAGGAAACAGTTGCAGAGCTAAGAACTGTTGAGAGTGAAGCTG
  CATCTTATCTGGACCAGATTTCTAGATATTATATTACAGGAGCCAAATTTGATTTCTAAA
  AATAGCTAAATATCCATGTGGGAGGACTATTCGCCCGCACCCGTGACAGAGATTGAATGA
  AAAGAATATTATTCAGGCTCCGCTCATCATATCCGAGCGTGAGATAGAATTTGTAACTCT
  ACATGACCTGCTATCTCTGGCAAATATCGGGAGAGTAGTATACAAAC
• Restriction Sites: Please inquire
• ACCN: NM_005789
• ORF Size: 765 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_005789.2, NP_005780.2
• RefSeq Size: 3189
• RefSeq ORF: 765
• Locus ID: 10197
• Domains: PA28_alpha, PA28_beta
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.