MMP2 (NM_004530) Human Untagged Clone

CAT#: SC321560

MMP2 (untagged)-Human matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) (MMP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMP2
• Synonyms: CLG4; CLG4A; MMP-2; MMP-II; MONA; TBE-1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004530.1 CTCAGGCGGTGGCTGGAGGCTGCGCATCTGGGGCTTTAAACATACAAAGGGATTGCCAGG
  ACCTGCGGCGGCGGCGGCGGCGGCGGGGGCTGGGGCGCGGGGGCCGGACCATGAGCCGCT
  GAGCCGGGCAAACCCCAGGCCACCGAGCCAGCGGACCCTCGGAGCGCAGCCCTGCGCCGC
  GGAGCAGGCTCCAACCAGGCGGCGACGCGGCCACACGCACCGAGCCAGCGACCCCCGGGC
  GACGCGCGGGGCCAGGGAGCGCTACGATGGAGGCGCTAATGGCCCGGGGCGCGCTCACGG
  GTCCCCTGAGGGCGCTCTGTCTCCTGGGCTGCCTGCTGAGCCACGCCGCCGCCGCGCCGT
  CGCCCATCATCAAGTTCCCCGGCGATGTCGCCCCCAAAACGGACAAAGAGTTGGCAGTGC
  AATACCTGAACACCTTCTATGGCTGCCCCAAGGAGAGCTGCAACCTGTTTGTGCTGAAGG
  ACACACTAAAGAAGATGCAGAAGTTCTTTGGACTGCCCCAGACAGGTGATCTTGACCAGA
  ATACCATCGAGACCATGCGGAAGCCACGCTGCGGCAACCCAGATGTGGCCAACTACAACT
  TCTTCCCTCGCAAGCCCAAGTGGGACAAGAACCAGATCACATACAGGATCATTGGCTACA
  CACCTGATCTGGACCCAGAGACAGTGGATGATGCCTTTGCTCGTGCCTTCCAAGTCTGGA
  GCGATGTGACCCCACTGCGGTTTTCTCGAATCCATGATGGAGAGGCAGACATCATGATCA
  ACTTTGGCCGCTGGGAGCATGGCGATGGATACCCCTTTGACGGTAAGGACGGACTCCTGG
  CTCATGCCTTCGCCCCAGGCACTGGTGTTGGGGGAGACTCCCATTTTGATGACGATGAGC
  TATGGACCTTGGGAGAAGGCCAAGTGGTCCGTGTGAAGTATGGCAACGCCGATGGGGAGT
  ACTGCAAGTTCCCCTTCTTGTTCAATGGCAAGGAGTACAACAGCTGCACTGATACCGGCC
  GCAGCGATGGCTTCCTCTGGTGCTCCACCACCTACAACTTTGAGAAGGATGGCAAGTACG
  GCTTCTGTCCCCATGAAGCCCTGTTCACCATGGGCGGCAACGCTGAAGGACAGCCCTGCA
  AGTTTCCATTCCGCTTCCAGGGCACATCCTATGACAGCTGCACCACTGAGGGCCGCACGG
  ATGGCTACCGCTGGTGCGGCACCACTGAGGACTACGACCGCGACAAGAAGTATGGCTTCT
  GCCCTGAGACCGCCATGTCCACTGTTGGTGGGAACTCAGAAGGTGCCCCCTGTGTCTTCC
  CCTTCACTTTCCTGGGCAACAAATATGAGAGCTGCACCAGCGCCGGCCGCAGTGACGGAA
  AGATGTGGTGTGCGACCACAGCCAACTACGATGACGACCGCAAGTGGGGCTTCTGCCCTG
  ACCAAGGGTACAGCCTGTTCCTCGTGGCAGCCCACGAGTTTGGCCACGCCATGGGGCTGG
  AGCACTCCCAAGACCCTGGGGCCCTGATGGCACCCATTTACACCTACACCAAGAACTTCC
  GTCTGTCCCAGGATGACATCAAGGGCATTCAGGAGCTCTATGGGGCCTCTCCTGACATTG
  ACCTTGGCACCGGCCCCACCCCCACACTGGGCCCTGTCACTCCTGAGATCTGCAAACAGG
  ACATTGTATTTGATGGCATCGCTCAGATCCGTGGTGAGATCTTCTTCTTCAAGGACCGGT
  TCATTTGGCGGACTGTGACGCCACGTGACAAGCCCATGGGGCCCCTGCTGGTGGCCACAT
  TCTGGCCTGAGCTCCCGGAAAAGATTGATGCGGTATACGAGGCCCCACAGGAGGAGAAGG
  CTGTGTTCTTTGCAGGGAATGAATACTGGATCTACTCAGCCAGCACCCTGGAGCGAGGGT
  ACCCCAAGCCACTGACCAGCCTGGGACTGCCCCCTGATGTCCAGCGAGTGGATGCCGCCT
  TTAACTGGAGCAAAAACAAGAAGACATACATCTTTGCTGGAGACAAATTCTGGAGATACA
  ATGAGGTGAAGAAGAAAATGGATCCTGGCTTTCCCAAGCTCATCGCAGATGCCTGGAATG
  CCATCCCCGATAACCTGGATGCCGTCGTGGACCTGCAGGGCGGCGGTCACAGCTACTTCT
  TCAAGGGTGCCTATTACCTGAAGCTGGAGAACCAAAGTCTGAAGAGCGTGAAGTTTGGAA
  GCATCAAATCCGACTGGCTAGGCTGCTGAGCTGGCCCTGGCTCCCACAGGCCCTTCCTCT
  CCACTGCCTTCGATACACCGGGCCTGGAGAACTAGAGAAGGACCCGGAGGGGCCTGGCAG
  CCGTGCCTTCAGCTCTACAGCTAATCAGCATTCTCACTCCTACCTGGTAATTTAAGATTC
  CAGAGAGTGGCTCCTCCCGGTGCCCAAGAATAGATGCTGACTGTACTCCTCCCAGGCGCC
  CCTTCCCCCTCCAATCCCACCAACCCTCAGAGCCACCCCTAAAGAGATCCTTTGATATTT
  TCAACGCAGCCCTGCTTTGGGCTGCCCTGGTGCTGCCACACTTCAGGCTCTTCTCCTTTC
  ACAACCTTCTGTGGCTCACAGAACCCTTGGAGCCAATGGAGACTGTCTCAAGAGGGCACT
  GGTGGCCCGACAGCCTGGCACAGGGCAGTGGGACAGGGCATGGCCAGGTGGCCACTCCAG
  ACCCCTGGCTTTTCACTGCTGGCTGCCTTAGAACCTTTCTTACATTAGCAGTTTGCTTTG
  TATGCACTTTGTTTTTTTCTTTGGGTCTTGTTTTTTTTTTCCACTTAGAAATTGCATTTC
  CTGACAGAAGGACTCAGGTTGTCTGAAGTCACTGCACAGTGCATCTCAGCCCACATAGTG
  ATGGTTCCCCTGTTCACTCTACTTAGCATGTCCCTACCGAGTCTCTTCTCCACTGGATGG
  AGGAAAACCAAGCCGTGGCTTCCCGCTCAGCCCTCCCTGCCCCTCCCTTCAACCATTCCC
  CATGGGAAATGTCAACAAGTATGAATAAAGACACCTACTGAGTGAAAAAAAAAAAAAAAA
  AAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004530.1 unedited
  GACGTTTGTATACGACTCCTATAGGGCGGGCGGAAATTCGGCACGAGGCTCAGGCGGTGG
  CTGGAGGCTGCGCATCTGGGGCTTTAAACATACAAAGGGATTGCCAGGACCTGCGGCGGC
  GGCGGCGGCGGCGGGGGCTGGGGCGCGGGGGCCGGACCATGAGCCGCTGAGCCGGGCAAA
  CCCCAGGCCACCGAGCCAGCGGACCCTCGGAGCGCAGCCCTGCGCCGCGGAGCAGGCTCC
  AACCAGGCGGCGACGCGGCCACACGCACCGAGCCAGCGACCCCCGGGCGACGCGCGGGGC
  CAGGGAGCGCTACGATGGTTTTTTTTTATGGCCCGGGGCGCGCTCACGGGTCCCCTGAGG
  GCGCTCTGTCTCCTGGGCTGCCTGCTGAGCCACGCCGCCGCCGCGCCGTCGCCCATCATC
  AAGTTCCCCGGCGATGTCGCCCCCAAAACGGACAAAGAGTTGGCAGTGCAATACCTGAAC
  ACCTTCTATGGCTGCCCCAAGGAGAGCTGCAACCTGTTTGTGCTGAAGGACACACTAAAG
  AAGATGCAGAAGTTCTTTGGACTGCCCCAGACAGGTGATCTTGACCAGAATACCATCGAG
  ACCATGCGGAAGCCACGCTGCGGCAACCCAGATGTGGCCAACTACAACTTCTTCCCTCGC
  AAGCCCAAGTGGGACAAGAACCAGATCACATACAGGATCATTGGCTACACACCTGATCTG
  GACCCAGAGACAGTGGATGATGCCTTTGCTCGTGCCTTTCCAAGTCTGGAGCGATGTGAC
  CCCCACTGGCGGTTTCTCGAATCCATGATGGAGAGGCAGACTTCATGATCAACTTTGGCC
  CGCTGGGAGCATGGCGATGGATACCCCTTGACGGTAAGACGACTCTGCTCATGCCTTTCG
  CCAGGCACTGGGTGGTTGGGGGGGAGAACTTCCAATAG
• Restriction Sites: Please inquire
• ACCN: NM_004530
• ORF Size: 1983 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_004530.1, NP_004521.1
• RefSeq Size: 3069
• RefSeq ORF: 1983
• Locus ID: 4313
• Domains: FN2, hemopexin, Peptidase_M10, ZnMc
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Bladder cancer, GnRH signaling pathway, Leukocyte transendothelial migration, Pathways in cancer
• Gene Summary: This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).