ISCU (NM_213595) Human Untagged Clone

CAT#: SC321619

ISCU (untagged)-Human iron-sulfur cluster scaffold homolog (E. coli) (ISCU), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ISCU
• Synonyms: 2310020H20Rik; HML; hnifU; ISU2; NIFU; NIFUN
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_213595.1 GGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTGGCCGTCTGAGGCGGGTGGCATCGGC
  TCTGCTGCTGCGGAGCCCCCGCCTGCCCGCCCGGGAGCTGTCGGCCCCGGCCCGACTCTA
  TCACAAGAAGGTTGTTGATCATTATGAAAATCCTAGAAACGTGGGGTCCCTTGACAAGAC
  ATCTAAAAATGTTGGAACTGGACTGGTGGGGGCTCCAGCATGTGGTGACGTAATGAAATT
  ACAGATTCAAGTGGATGAAAAGGGGAAGATTGTGGATGCTAGGTTTAAAACATTTGGCTG
  TGGTTCCGCAATTGCCTCCAGCTCATTAGCCACTGAATGGGTGAAAGGAAAGACGGTGGA
  GGAAGCCTTGACTATCAAAAACACAGATATCGCCAAGGAGCTCTGCCTTCCTCCCGTGAA
  ACTGCACTGCTCCATGCTGGCTGAAGATGCAATCAAGGCCGCCCTGGCTGATTACAAATT
  GAAACAAGAACCCAAAAAAGGAGAGGCAGAGAAGAAATGAGCCCTCCCTCGGCGAAGCCT
  CCAGCAGGCCACACCAGCTGTTTCCCACCTGCTGTGCAGTCACCTTAGATGTTCAGAAGC
  CGCTTCCTCTCCACTGAAGAGCTATGAGATACGCACAATACTTGCTGTTCACGTTATGAC
  TCTCATGCAAGCAAAATACACAGTTTCATTGTTCTGAATCCTGTGGTTTCTTTCAGCCCA
  CTTTTATCGCCTTAACCTAGTTAATGTATATTTTGAATTGTGTGTATGACCTCAGAACTG
  AAATTGATAATGAAGTTGCAAGTTTTGATAGCCCGTGAAGTGCATAAGTATCTAATTTTA
  CCTGAATTGATTTGGGGGGAAATTACCAGTAGAATGCCTTGGTCTGAATATTTGATAGAA
  CCAATTGTTGTACATAAAACAGATCTGCGCATATATATATATGTATAAAAAATAATAAAA
  TAATGGAAGATGAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_213595.1 unedited
  GGCCATTGTATACGACTCCTATAGGGCGGCGCGGGAATTCGGCACGAGGGGCGCAAGCCG
  GCAAGATGGCGGCGGCTGGGGCTGGCCGTCTGAGGCGGGTGGCATCGGCTCTGCTGCTGC
  GGAGCCCCCGCCTGCCCGCCCGGGAGCTGTCGGCCCCGGCCCGACTCTATCACAAGAAGG
  TTGTTGATCATTATGAAAATCCTAGAAACGTGGGGTCCCTTGACAAGACATCTAAAAATG
  TTGGAACTGGACTGGTGGGGGCTCCAGCATGTGGTGACGTAATGAAATTACAGATTCAAG
  TGGATGAAAAGGGGAAGATTGTGGATGCTAGGTTTAAAACATTTGGCTGTGGTTCCGCAA
  TTGCCTCCAGCTCATTAGCCACTGAATGGGTGAAAGGAAAGACGGTGGAGGAAGCCTTGA
  CTATCAAAAACACAGATATCGCCAAGGAGCTCTGCCTTCCTCCCGTGAAACTGCACTGCT
  CCATGCTGGCTGAAGATGCAATCAAGGCCGCCCTGGCTGATTACAAATTGAAACAAGAAC
  CCAAAAAAGGAGAGGCAGAGAAGAAATGAGCCCTCCCTCGGCGAAGCCTCCAGCAGCCAC
  ACCAGCTGTTTCCCACCTGCTGTGCAGTCACCTTAGATGTTCAGAAGCCGCTTCCTCTCC
  ACTGAAGAGCTATGAGATACGCACAATACTTGCTGTTCACGTTATGACTCTCATGCAAGC
  AAAATACACAGTTTCATTTGTTCTGAATCCTGTGGTTTCTTTCAGCCCACTTTTTATCGC
  CTTACCCTAGTTAATGTATATTTTTGAATTGTGTTGTATGACCTCAGACTGAAATTGAAT
  AATGAAGTTGCAGTTTTGATAGCCGTGAAGTGCATAAGTATCTATTTACTGATGATTGGG
  GAATACAGTGAATGCTGTCTGATATTAGATGAACATTGTTGTACTAACGATTCCTGCGCG
  CCAAAT
• Restriction Sites: Please inquire
• ACCN: NM_213595
• ORF Size: 504 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_213595.1, NP_998760.1
• RefSeq Size: 990
• RefSeq ORF: 504
• Locus ID: 23479
• Gene Summary: This gene encodes a component of the iron-sulfur (Fe-S) cluster scaffold. Fe-S clusters are cofactors that play a role in the function of a diverse set of enzymes, including those that regulate metabolism, iron homeostasis, and oxidative stress response. Alternative splicing results in transcript variants encoding different protein isoforms that localize either to the cytosol or to the mitochondrion. Mutations in this gene have been found in patients with hereditary myopathy with lactic acidosis. A disease-associated mutation in an intron may activate a cryptic splice site, resulting in the production of a splice variant encoding a putatively non-functional protein. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (2) encodes the longest isoform (2). Isoform 2 is localized to the mitochondrion.