PSMD5 (NM_005047) Human Untagged Clone

CAT#: SC321629

PSMD5 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 (PSMD5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD5
• Synonyms: S5B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005047.2 GATGGCAGCCCAGGCTTTGGCGCTGCTGAGAGAGGTAGCGAGGCTGGAAGCGCCGCTGGA
  GGAGCTACGCGCGCTTCACTCCGTGCTGCAGGCAGTGCCGCTCAACGAGCTTCGCCAGCA
  AGCGGCGGAGCTGCGCCTCGGCCCGCTCTTCTCCCTGCTTAACGAGAACCATAGGGAAAA
  GACTACTTTGTGTGTATCCATTCTGGAGAGATTGCTCCAAGCTATGGAACCGGTTCACGT
  GGCCCGGAACCTCAGGGTTGACCTGCAGAGGGGACTAATTCACCCTGATGATTCTGTAAA
  AATCCTCACTCTTTCCCAGATTGGAAGAATTGTTGAAAATTCTGATGCTGTTACTGAGAT
  TCTAAATAATGCTGAATTACTAAAACAAATTGTTTATTGCATTGGTGGAGAGAATCTATC
  TGTAGCAAAAGCGGCTATCAAATCCCTGTCAAGAATATCACTAACCCAAGCTGGACTGGA
  GGCTTTATTTGAAAGCAATCTGCTGGATGATTTGAAAAGTGTAATGAAAACAAATGACAT
  TGTTCGATACAGGGTGTATGAGCTAATTATAGAGATTTCTTCCGTGTCACCAGAATCTTT
  AAACTACTGTACCACAAGTGGATTGGTAACCCAGCTCCTGAGAGAGCTGACTGGTGAGGA
  TGTGTTGGTCAGAGCCACCTGTATAGAAATGGTGACATCACTGGCATATACTCATCATGG
  GCGACAATATCTTGCTCAAGAAGGAGTAATTGACCAAATTTCTAATATAATTGTTGGGGC
  AGATTCAGACCCTTTCTCTAGCTTCTATCTGCCAGGATTCGTGAAGTTTTTTGGAAACCT
  GGCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTTGTGGAAAAAGT
  CTTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTAGACACAGTTGG
  AATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGAACTCGCTTTGA
  ACGCTTGCTTATGAGAATAGGACATCAATCAAAGAATGCCCCAGTGGAGCTAAAAATTAG
  ATGTTTGGATGCAATTTCATCTCTTCTGTACTTACCACCTGAGCAGCAGACTGATGACCT
  TCTGAGGATGACAGAATCCTGGTTTTCTTCTTTATCTCGGGATCCACTGGAGCTCTTCCG
  TGGCATTAGTAGTCAGCCCTTCCCTGAACTACACTGTGCTGCCTTAAAAGTGTTTACGGC
  CATTGCAAACCAACCCTGGGCTCAGAAACTTATGTTTAACAGTCCAGGTTTTGTAGAATA
  TGTGGTGGACCGGTCTGTGGAGCATGACAAAGCTTCAAAGGATGCCAAATATGAACTAGT
  GAAAGCACTTGCCAATTCCAAGACAATTGCAGAAATCTTTGGGAACCCAAATTATTTGAG
  GCTCAGAACTTACCTGAGTGAAGGGCCATACTATGTGAAACCTGTTTCCACGACAGCAGT
  AGAAGGAGCCGAATGATTTCTTCTAGAGCTCATCTATAGAGGACCACGTTTTGACCAAAA
  CTTCTCCTAAGGCATTTGACTCCATCTATATTTCACAAAAGAGACTTCCTTTCCCCAAGA
  ATTATCATGGAATGTCAGATGTTACTTTGTTACCAACACTGTTATATTTCTACATTGAAA
  TGCAAAGTGGAACTAGGAGTTTGGAATGCATTAAGAGCAGACAAGCTTGGTCATAATAGA
  TCCAGTGTTTTTCAGATTCCTTTCACTGCCTTAATCTTTGCAACAGGGTGGAAGTTTTTT
  TCTTCCCTCAAAATTTTCATGGACATGCAATCTTATCTAAAAGCCTGCTTCAGGGCTGGG
  CGCGGTGGCTAACACCTATAATTCCCAGCACTTTGGGAGGCCAAGATGGGCAAATCACTT
  GAGTCTAGGAGTTCAAGACCAGCCTGGCCAATATGGCAAAACCCTGTCTCTACTAAAAAT
  ATAAAATCAGCCAGGCATGGTGGCGCACACCTATAATCTCAGCTACTCAGGAGGCTGAGG
  CACGAGAATCGCTTGAGCCTGGGAGGCAGAGGTTGCAGTGAGCTGAGATCTTGCCACTGC
  ACACCAGCCTGGGCAACACAGCAAGACTCTGTCTCAAAAATAAATGAATAAAATAAAAAC
  CTGCCACCAAATTATTTCTGGATTCTCTTCACTATCTTTTTTTTTCTTTTTGGTCCTTTT
  TCCATTTCTGATTCCACTTTCAAAGTCTTGGATATATTCTTTGAAAAATGAGTAGTATGG
  AATATTAAGATTATGTTCCTTATACTCAGAATACTTTTTATGAGCTCTCTTGTTTTTTTG
  TTACTATTATTCCTTTGAAAAAAAGACAAAATATCTAAAAAATGTTTATAGTGTTCATGT
  AATTTTTTTTGCTGTTTACCAATGAACTTTGCCCTGTTGTGGTTTTGGGGGATCAGAAGG
  ATTCCTAAACTGTCATTTCATTTATTGGTAGGTCATGATTGCTGAGGGTTTTGCTTTGGA
  TTTTTCATACCTATAAAGTCAATCTTGAGTTGATCAGAGAAAGGGAAACTTACTCCTAAT
  AAACAGACTCAGAACAGAAGGGACATATGCATAAATCAGGATACCATCTCATTAATTTAC
  AAAGGATAATCTTTCTTTTCATTGACAACACCTACACCCACCTGTTTTCCAGTGTTCTTC
  TCTCTAAACAGCAAAGCCAAAGCACTCTGCACACCAAATCACCTGTTCAGAGGTTTATGC
  ATGAGCATATTTCTTTTAGGCCGTGGTGAAGTTGATAAACCACCCCTGCTTTGTAACAAA
  ATTATTTTAAAGTGACTGGAATTATCTAGTCCCCAGATTGATCATCTCCCCTGGCAACGT
  GACTCTGTTTTTTGTGTGTGTTTCCATGCTGACTAGTCCCCTACTGTTAATATCACTACT
  AATTAGGCTATAACCAGGTCTTTCCTGGCCTGAGAAATATTCTCTTAAAATGACCTTTGT
  TTTAATCTCATTCATGATGTTGATTTTTTTTCAATGTGGTGCAATATATACAATAAAATT
  TGTCATAACTATTTTAAGTGTACAGTTCAGTATATTACATATGTTCATAAGTTGTACAAC
  CATCACCACCATCCATCTTCAGAATTATTTTCATCTTATACAATTGAAACTGTATTCATT
  AAACAATAACTGTCTATTCCCTCTTTTCCCCAGCCTCTGGCAACCACCATTCTGCTTCAT
  GACATTGATTTTATTCACTTAACATCAGATTTTGGGGGGATGCCCTCTCTCCTATGTATG
  GTCTACACATTCTGATTTTTTTTTTAGATTGAATAAAATTTGTAAATCATTATTTTCATT
  TCATAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005047
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005047.2, NP_005038.1
• RefSeq Size: 3411 bp
• RefSeq ORF: 1515 bp
• Locus ID: 5711
• Cytogenetics: 9q33.2
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).