PDK1 (NM_002610) Human Untagged Clone

CAT#: SC321678

PDK1 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDK1
• Synonyms: mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002610.3 GAAACCTGGCGTACTGGCTGTGGCTTCTCTAGCGGGACTCGGCATGAGGCTGGCGCGGCT
  GCTTCGCGGAGCCGCCTTGGCCGGCCCGGGCCCGGGGCTGCGCGCCGCCGGCTTCAGCCG
  CAGCTTCAGCTCGGACTCGGGCTCCAGCCCGGCGTCCGAGCGCGGCGTTCCGGGCCAGGT
  GGACTTCTACGCGCGCTTCTCGCCGTCCCCGCTCTCCATGAAGCAGTTCCTGGACTTCGG
  ATCAGTGAATGCTTGTGAAAAGACCTCATTTATGTTTCTGCGGCAAGAGTTGCCTGTCAG
  ACTGGCAAATATAATGAAAGAAATAAGTCTCCTTCCAGATAATCTTCTCAGGACACCATC
  CGTTCAATTGGTACAAAGCTGGTATATCCAGAGTCTTCAGGAGCTTCTTGATTTTAAGGA
  CAAAAGTGCTGAGGATGCTAAAGCTATTTATGACTTTACAGATACTGTGATACGGATCAG
  AAACCGACACAATGATGTCATTCCCACAATGGCCCAGGGTGTGATTGAATACAAGGAGAG
  CTTTGGGGTGGATCCTGTCACCAGCCAGAATGTTCAGTACTTTTTGGATCGATTCTACAT
  GAGTCGCATTTCAATTAGAATGTTACTCAATCAGCACTCTTTATTGTTTGGTGGAAAAGG
  CAAAGGAAGTCCATCTCATCGAAAACACATTGGAAGCATAAATCCAAACTGCAATGTACT
  TGAAGTTATTAAAGATGGCTATGAAAATGCTAGGCGTCTGTGTGATTTGTATTATATTAA
  CTCTCCCGAACTAGAACTTGAAGAACTAAATGCAAAATCACCAGGACAGCCAATACAAGT
  GGTTTATGTACCATCCCATCTCTATCACATGGTGTTTGAACTTTTCAAGAATGCAATGAG
  AGCCACTATGGAACACCATGCCAACAGAGGTGTTTACCCCCCTATTCAAGTTCATGTCAC
  GCTGGGTAATGAGGATTTGACTGTGAAGATGAGTGACCGAGGAGGTGGCGTTCCTTTGAG
  GAAAATTGACAGACTTTTCAACTACATGTATTCAACTGCACCAAGACCTCGTGTTGAGAC
  CTCCCGCGCAGTGCCTCTGGCTGGTTTTGGTTATGGATTGCCCATATCACGTCTTTACGC
  ACAATACTTCCAAGGAGACCTGAAGCTGTATTCCCTAGAGGGTTACGGGACAGATGCAGT
  TATCTACATTAAGGCTCTGTCAACAGACTCAATAGAAAGACTCCCAGTGTATAACAAAGC
  TGCCTGGAAGCATTACAACACCAACCACGAGGCTGATGACTGGTGCGTCCCCAGCAGAGA
  ACCCAAAGACATGACGACGTTCCGCAGTGCCTAGACACACTTGGGACATCGGAAAATCCA
  AATGTGGCTTTTGTATTAAATTTGGAAGTGTGGCCCAGAGTTGCTCAGAATTGGAGCAGA
  GCCTGAGACGTATCTGCAGATCCTGTCATCAGCTGGCAAGTCCAGGAGACTGTGTCATTT
  AGAGACTGTGTTGTTAGTTATCCCTCAACATCTTCTAAGGTGGCAGGAAATAATATTGGA
  AATAACATTTTAAAGTAAAAATTTTAAAGTTTAAAGAAGAGTTTTGCCACTTAAACAGGG
  GAGCTTTGTCTGGAAAATACACTGAGTTGAAACACTTCATCCTTGGAAGGATTATATAAG
  ATGAACAGTTGTGATAAATGTGTAGATTAGAGGGATGTGAATGGGCAGTTAGTCCAGTGC
  CCTCATTTAAGAGGCCAAGATCCTGATTCAGAGGAGGCATCCTTTGCCCAGAGCTGCTTA
  GCTAATCTGACCAAATGTTGGGAAAAATGTCTCACCTAACCCACTATTCCTTAATTATGG
  ATTTTGTGAAAAACAATAGAACATGTTAATGAGTAATTTATATTAGTTCGATGTATTACA
  ATTTTTTAGCTTTAAATTACAGTTTTCTTATAATGTTGAAATGTTTTAGAATCCTTTGAA
  TCTAAGTATTTGTTTCCTAAATGAAACATTTGTACAACATTTGATGTTTTTACTTATGAA
  ATATTCTCCTCCCCCAAGAAAATTTAAACTTTTTCTCTCTATTTAAAAGCTAAGAAATGT
  TTTAAAGGAAAAATGAAATTATCTTCCTTTAGCTTATTTTTAAGGTAAAACAGCTTTTTA
  CTCTGTTATTGTGGTAATGGACAGAATATTACATACAAAAATATTCTGGGAGAGCTTTTT
  CCTAGTTGGTTTTAAATCATTGTGCCACCTGAAAGGTTTTTAGATTTTATAGGAGCTAAT
  TTGTCCACCAGCATTAATGTAACACAGTGTAGTTATGAAAATATATTGAAGGACAGGAAG
  TGGACACGAAGTGATTTTTGTAACCTGAGCAGTTAATGAATGTGCCAACATTTTCTAGGA
  AGGGACAGCAAGAATATTCTGCTCTGTAGTTAAAATACTGGCTGGCTTTTGATGTCTTCA
  TGCTTAATTGTGATCACTTTCTTGCACTGTGATGTTTTTACGTGAATATGTTGAAGTAGA
  AGTCTACCATATTATTTTATAAAATGTTTTCTGTATGGCAATAAACTGAAAACATGGATC
  AACCCTTCTTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002610
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002610.3, NP_002601.1
• RefSeq Size: 4576 bp
• RefSeq ORF: 1311 bp
• Locus ID: 5163
• Cytogenetics: 2q31.1
• Domains: HATPase_c
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Fc epsilon RI signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway
• Gene Summary: 'Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.