Pyruvate Kinase (PKLR) (NM_000298) Human Untagged Clone

CAT#: SC321691

PKLR (untagged)-Human pyruvate kinase, liver and RBC (PKLR), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKLR
• Synonyms: PK1; PKL; PKRL; RPK
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000298.4 GCAGCCCCAGGCCCACACTGAAAGCATGTCGATCCAGGAGAACATATCATCCCTGCAGCT
  TCGGTCATGGGTCTCTAAGTCCCAAAGAGACTTAGCAAAGTCCATCCTGATTGGGGCTCC
  AGGAGGGCCAGCGGGGTATCTGCGGCGGGCCAGTGTGGCCCAACTGACCCAGGAGCTGGG
  CACTGCCTTCTTCCAGCAGCAGCAGCTGCCAGCTGCTATGGCAGACACCTTCCTGGAACA
  CCTCTGCCTACTGGACATTGACTCCGAGCCCGTGGCTGCTCGCAGTACCAGCATCATTGC
  CACCATCGGGCCAGCATCTCGCTCCGTGGAGCGCCTCAAGGAGATGATCAAGGCCGGGAT
  GAACATTGCGCGACTCAACTTCTCCCACGGCTCCCACGAGTACCATGCTGAGTCCATCGC
  CAACGTCCGGGAGGCGGTGGAGAGCTTTGCAGGTTCCCCACTCAGCTACCGGCCCGTGGC
  CATCGCCCTGGACACCAAGGGACCGGAGATCCGCACTGGGATCCTGCAGGGGGGTCCAGA
  GTCGGAAGTGGAGCTGGTGAAGGGCTCCCAGGTGCTGGTGACTGTGGACCCCGCGTTCCG
  GACGCGGGGGAACGCGAACACCGTGTGGGTGGACTACCCCAATATTGTCCGGGTCGTGCC
  GGTGGGGGGCCGCATCTACATTGACGACGGGCTCATCTCCCTAGTGGTCCAGAAAATCGG
  CCCAGAGGGACTGGTGACCCAAGTGGAGAACGGCGGCGTCCTGGGCAGCCGGAAGGGCGT
  GAACTTGCCAGGGGCCCAGGTGGACTTGCCCGGGCTGTCCGAGCAGGACGTCCGAGACCT
  GCGCTTCGGGGTGGAGCATGGGGTGGACATCGTCTTTGCCTCCTTTGTGCGGAAAGCCAG
  CGACGTGGCTGCCGTCAGGGCTGCTCTGGGTCCGGAAGGACACGGCATCAAGATCATCAG
  CAAAATTGAGAACCACGAAGGCGTGAAGAGGTTTGATGAAATCCTGGAGGTGAGCGACGG
  CATCATGGTGGCACGGGGGGACCTAGGCATCGAGATCCCAGCAGAGAAGGTTTTCCTGGC
  TCAGAAGATGATGATTGGGCGCTGCAACTTGGCGGGCAAGCCTGTTGTCTGTGCCACACA
  GATGCTGGAGAGCATGATTACCAAGCCCCGGCCAACGAGGGCAGAGACAAGCGATGTCGC
  CAATGCTGTGCTGGATGGGGCTGACTGCATCATGCTGTCAGGGGAGACTGCCAAGGGCAA
  CTTCCCTGTGGAAGCGGTGAAGATGCAGCATGCGATTGCCCGGGAGGCAGAGGCCGCAGT
  GTACCACCGGCAGCTGTTTGAGGAGCTACGTCGGGCAGCGCCACTAAGCCGTGATCCCAC
  TGAGGTCACCGCCATTGGTGCTGTGGAGGCTGCCTTCAAGTGCTGTGCTGCTGCCATCAT
  TGTGCTGACCACAACTGGCCGCTCAGCCCAGCTTCTGTCTCGGTACCGACCTCGGGCAGC
  AGTCATTGCTGTCACCCGCTCTGCCCAGGCTGCCCGCCAGGTCCACTTATGCCGAGGAGT
  CTTCCCCTTGCTTTACCGTGAACCTCCAGAAGCCATCTGGGCAGATGATGTAGATCGCCG
  GGTGCAATTTGGCATTGAAAGTGGAAAGCTCCGTGGCTTCCTCCGTGTTGGAGACCTGGT
  GATTGTGGTGACAGGCTGGCGACCTGGCTCCGGCTACACCAACATCATGAGGGTGCTAAG
  CATATCCTGAGACGCCCCTCCCCCCTCTGGCCCAGCCTACCCTTGTACCCCATCCCTTCC
  TCCCCAGTCTACGTTCTCCAGCCCACACCCCTCCAAAGCCCCACCTTTAAGTCCTCTCTT
  CTCTATTCCTGACCCTCCCTACCTGAGGCCTATCTGAGACTATAACTGTCATCTAGCCCC
  TTCGAGGTTGCCCCTTCCCCATCTCCATTTCACACAGGTCCTGAAAGTCTGTGTCCAATT
  ATGCACTGGCCACCCAACAGCACCAATTGTACATTCTCTGCATCCAATCTGCTCAGCAGG
  CCCTAAGATGCCTTGAGTCTTTAATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000298
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000298.4, NP_000289.1
• RefSeq Size: 2483 bp
• RefSeq ORF: 1725 bp
• Locus ID: 5313
• Cytogenetics: 1q22
• Domains: PK
• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus
• Gene Summary: 'The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Isoform 1 is also known as type R. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.