Pepsin (PGA5) (NM_014224) Human Untagged Clone

CAT#: SC321793

PGA5 (untagged)-Human pepsinogen 5, group I (pepsinogen A) (PGA5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PGA5
• Synonyms: Pg5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014224.1 TCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCTGGGTCTGGTG
  GCGCTCTCTGAGTGCATCATGTACAAGGTCCCCCTCATCAGAAAGAAGTCCTTGAGGCGC
  ACCCTGTCCGAGCGTGGCCTGCTGAAGGACTTCCTGAAGAAGCACAACCTCAACCCAGCC
  AGAAAGTACTTCCCCCAGTGGGAGGCTCCCACCCTGGTAGATGAACAGCCCCTGGAGAAC
  TACCTGGATATGGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGGATTTCACC
  GTCGTCTTTGACACCGGCTCCTCCAACCTGTGGGTGCCCTCAGTCTACTGCTCCAGTCTT
  GCCTGCACCAACCACAACCGCTTCAACCCTGAGGATTCTTCCACCTACCAGTCCACCAGC
  GAGACAGTCTCCATCACCTACGGCACCGGCAGCATGACAGGCATCCTCGGATACGACACT
  GTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGAGACGGAACCT
  GGCTCCTTCCTGTATTATGCTCCCTTCGATGGCATCCTGGGGCTGGCCTACCCCAGCATT
  TCCTCCTCCGGGGCCACACCCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAG
  GACCTCTTCTCTGTCTACCTCAGCGCCGATGACAAGAGTGGCAGCGTGGTGATCTTTGGT
  GGCATTGACTCTTCTTACTACACTGGAAGTCTGAACTGGGTGCCTGTTACCGTCGAGGGT
  TACTGGCAGATCACCGTGGACAGCATCACCATGAACGGAGAGACCATCGCCTGTGCTGAG
  GGCTGCCAGGCCATTGTTGACACCGGCACCTCTCTGCTGACCGGCCCAACCAGCCCCATT
  GCCAACATCCAGAGCGACATCGGAGCCAGCGAGAACTCAGATGGCGACATGGTGGTCAGC
  TGCTCAGCCATCAGCAGCCTGCCCGACATCGTCTTCACCATCAATGGAGTCCAGTACCCC
  GTGCCACCCAGTGCCTACATCCTGCAGAGCGAGGGGAGCTGCATCAGTGGCTTCCAGGGC
  ATGAACGTCCCCACCGAATCTGGAGAGCTTTGGATCCTGGGTGATGTCTTCATCCGCCAG
  TACTTTACCGTCTTCGACAGGGCAAACAACCAGGTCGGCCTGGCCCCTGTGGCTTAAGCC
  TAAGTCTCTTCAGCCACCTCCCAGGAAGATCTGGCCTCCGTCCTATGCCCACTTTAGATG
  TATCTAATTCTCCTGACTGTTCTTCCCAGGGGAGTGTGAAGGTCTTGGCCCTGTTCCCTG
  TCCTACCAATAACGTAGAATAAAAACATAACCCACTGAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014224
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014224.1, NP_055039.1
• RefSeq Size: 1387 bp
• RefSeq ORF: 1167 bp
• Locus ID: 5222
• Cytogenetics: 11q12.2
• Domains: asp
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]'