DPAGT1 (NM_203316) Human Untagged Clone

CAT#: SC321827

DPAGT1 (untagged)-Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPAGT1
• Synonyms: GPT, ALG7, DGPT, G1PT, UAGT, UGAT, DPAGT, CDG-Ij, DPAGT2, D11S366
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_203316.1 CCGGTGTCCCGACGGCGGCTCAAGTCAGAGTTGCTGGGTTTTGCTCAGATTGGTGTGGGA
  AGAGCCTGCCTGTGGGGAGCGGCCACTCCATACTGCTGAGGCCTCAGGACTGCTGCTCAG
  CTTGCCCGTTACCTGAAGAGGCGGCGGAGCCGGGCCCCTGACCGGTCACCATGTGGGCCT
  TCTCGGAATTGCCCATGCCGCTGCTGATCAATTTGATCGTCTCGCTGCTGGGATTTGTGG
  CCACAGTCACCCTCATCCCGGCCTTCCGGGGCCACTTCATTGCTGCGCGCCTCTGTGGTC
  AGGACCTCAACAAAACCAGCCGACAGCAGATTTTGTGGCCCTGATAGGTGCCCTCCTTGC
  CATCTGCTGCATGATCTTCCTGGGCTTTGCGGATGATGTACTGAATCTGCGCTGGCGCCA
  TAAGCTGCTGCTACCTACAGCTGCCTCACTACCTCTCCTCATGGTCTATTTCACCAACTT
  TGGCAACACGACCATTGTGGTGCCCAAGCCCTTCCGCCCGATACTTGGCCTGCATCTGGA
  CTTGGGAATCCTGTACTATGTCTACATGGGGCTGCTGGCAGTGTTCTGTACCAATGCCAT
  CAATATCCTAGCAGGAATTAACGGCCTAGAGGCTGGCCAGTCACTAGTCATTTCTGCTTC
  CATCATTGTCTTCAACCTGGTAGAGTTGGAAGGTGATTGTCGGGATGATCATGTCTTTTC
  CCTCTACTTCATGATACCCTTTTTTTTCACCACTTTGGGATTGCTCTACCACAACTGGTA
  CCCATCACGGGTGTTTGTGGGAGATACCTTCTGTTACTTTGCTGGCATGACCTTTGCCGT
  GGTGGGCATCTTGGGACACTTCAGCAAGACCATGCTACTATTCTTCATGCCCCAGGTGTT
  CAACTTCCTCTACTCACTGCCTCAGCTCCTGCATATCATCCCCTGCCCTCGCCACCGCAT
  ACCCAGACTCAATATCAAGACAGGCAAACTGGAGATGAGCTATTCCAAGTTCAAGACCAA
  GAGCCTCTCTTTCTTGGGCACCTTTATTTTAAAGGTGGCAGAGAGCCTCCAGCTGGTGAC
  AGTACACCAGAGTGAGACTGAAGATGGTGAATTCACTGAATGTAACAACATGACCCTCAT
  CAACTTGCTACTTAAAGTCCTTGGGCCCATACATGAGAGAAACCTCACATTGCTCCTGCT
  GCTGCTGCAGATCCTGGGCAGTGCCGTCACCTTCTCCATTCGATATCAGCTCGTTCGACT
  CTTCTATGATGTCTGAGTCCCTTGATCATTGTCCTTTACCTCACAGTCTCTAGGATTCCT
  GACTCAGGCTGACCTCTCTCTCTGGTCCCAGACTGCCTCCTTGCCCAGGCCTCTCTCACT
  CTTCATACTCCTCCAGATTTTGTTCTCAGCATTTTCCTTTCTCTGTGATCATTGGCATCC
  TGGGCGTTTCTTGCCCTCTACTGACTACTGATTGGATTTTACCTATGGCTTTCTGCAACT
  TGCTACTCTCTCCCTCTCCATCCCATCTTTGCAGCCTCATAGGGTGGGATACAGCAGCTT
  TTTTTGCAGTTATCCACACTCACATTTCAGAGTCCTGACTCTCAAGGAACCACTGGTTTT
  TGGGATAGAACTTGGGCCAGGGCTAGGAACACAGGCTCCACGGTGACATGTCATTTGATT
  GTAAATTAAGTGCTCTGATTAGTAAGAACTAAGCAGGGGGCCACATGCTCTCAATGGAGA
  CAATAAAGTGTTGTCTTTTTCTTAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_203316
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203316.1, NP_976061.1
• RefSeq Size: 2038 bp
• RefSeq ORF: 906 bp
• Locus ID: 1798
• Cytogenetics: 11q23.3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an in-frame segment, compared to variant 1. It causes translation initiation at a downstream ATG and an isoform (b) with a shorter N-terminus compared to isoform a.