Renin (REN) (NM_000537) Human Untagged Clone

CAT#: SC321832

REN (untagged)-Human renin (REN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: REN
• Synonyms: HNFJ2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000537.2 CTCAGAGAGAGCCCCAGACTGAGGGAAGCATGGATGGATGGAGAAGGATGCCTCGCTGGG
  GACTGCTGCTGCTGCTCTGGGGCTCCTGTACCTTTGGTCTCCCGACAGACACCACCACCT
  TTAAACGGATCTTCCTCAAGAGAATGCCCTCAATCCGAGAAAGCCTGAAGGAACGAGGTG
  TGGACATGGCCAGGCTTGGTCCCGAGTGGAGCCAACCCATGAAGAGGCTGACACTTGGCA
  ACACCACCTCCTCCGTGATCCTCACCAACTACATGGACACCCAGTACTATGGCGAGATTG
  GCATCGGCACCCCACCCCAGACCTTCAAAGTCGTCTTTGACACTGGTTCGTCCAATGTTT
  GGGTGCCCTCCTCCAAGTGCAGCCGTCTCTACACTGCCTGTGTGTATCACAAGCTCTTCG
  ATGCTTCGGATTCCTCCAGCTACAAGCACAATGGAACAGAACTCACCCTCCGCTATTCAA
  CAGGGACAGTCAGTGGCTTTCTCAGCCAGGACATCATCACCGTGGGTGGAATCACGGTGA
  CACAGATGTTTGGAGAGGTCACGGAGATGCCCGCCTTACCCTTCATGCTGGCCGAGTTTG
  ATGGGGTTGTGGGCATGGGCTTCATTGAACAGGCCATTGGCAGGGTCACCCCTATCTTCG
  ACAACATCATCTCCCAAGGGGTGCTAAAAGAGGACGTCTTCTCTTTCTACTACAACAGAG
  ATTCCGAGAATTCCCAATCGCTGGGAGGACAGATTGTGCTGGGAGGCAGCGACCCCCAGC
  ATTACGAAGGGAATTTCCACTATATCAACCTCATCAAGACTGGTGTCTGGCAGATTCAAA
  TGAAGGGGGTGTCTGTGGGGTCATCCACCTTGCTCTGTGAAGACGGCTGCCTGGCATTGG
  TAGACACCGGTGCATCCTACATCTCAGGTTCTACCAGCTCCATAGAGAAGCTCATGGAGG
  CCTTGGGAGCCAAGAAGAGGCTGTTTGATTATGTCGTGAAGTGTAACGAGGGCCCTACAC
  TCCCCGACATCTCTTTCCACCTGGGAGGCAAAGAATACACGCTCACCAGCGCGGACTATG
  TATTTCAGGAATCCTACAGTAGTAAAAAGCTGTGCACACTGGCCATCCACGCCATGGATA
  TCCCGCCACCCACTGGACCCACCTGGGCCCTGGGGGCCACCTTCATCCGAAAGTTCTACA
  CAGAGTTTGATCGGCGTAACAACCGCATTGGCTTCGCCTTGGCCCGCTGAGGCCCTCTGC
  CACCCAGGCAGGCCCTGCCTTCAGCCCTGGCCCAGAGCTGGAACACTCTCTGAGATGCCC
  CTCTGCCTGGGCTTATGCCCTCAGATGGAGACATTGGATGTGGAGCTCCTGCTGGATGCG
  TGCCCTGACCCCTGCACCAGCCCTTCCCTGCTTTGAGGACAAAGAGAATAAAGACTTCAT
  GTTCACAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000537
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000537.2, NP_000528.1
• RefSeq Size: 1461 bp
• RefSeq ORF: 1221 bp
• Locus ID: 5972
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Renin-angiotensin system
• Gene Summary: 'This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis. [provided by RefSeq, May 2020]'