PRAME (NM_206955) Human Untagged Clone

CAT#: SC321921

PRAME (untagged)-Human preferentially expressed antigen in melanoma (PRAME), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRAME
• Synonyms: CT130; MAPE; OIP-4; OIP4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_206955.1 GCCAGAAGCCGGGCCTGCAGCGCCTCAGCACCGCTCCGGGACACCCCACCCGCTTCCCAG
  GCGTGACCTGTCAACAGCAACTTCGCGGTGTGGTGAACTCTCTGAGGAAAAACGTAAGTT
  CGAGCCCTGATTCCTCCGCTTCCCCGCAGGGTGACCTTGGGCTTGTGCCCCCGGCACCAC
  CCCTGTCCCGGGTCCCTGTTTTCTCTCTGGAAATGGGTTGAAGACCAAAGAAAATAATGT
  GCGCCACTTGGGTCACCCCGGGCCGCCTGCCCCGGAAAATTGGCCCCAGTTGAGGAGTTG
  TGGCTGTAAGGATGCCTTGAACCGAGGCGGCGGTGCTCGTGGTTGGAGCTCTCCAGGGTG
  GGTGCGCATTTGTAATGCGGTGGATGCTCTGGGACTCGGCCCCTCTGAAGGTGCTTGGGG
  GTTGGGGACGGCCCAGGCAGCAGTGGCGTAGGCGTCCTAGGAAGGCGGGAGCAGAGGCAG
  AAATGTCGCTGCAAGACCGTAGTCAGGGTCCTTGACCACAGGGGTCACTTGTGACCAACC
  ACATGGTCTGTTGTTCCTCCTGCCCCCTGGTTCAGCCCAGGAAACACTGGTGCTCAGGTT
  TGGAGCCAGAGATTTGCACTGAAAGGGCGGGATTGAGTCGCCAGTTGTCAGTTTCCTCAG
  CAGTATTCGCGGAGGTTTTCACAGGAGGCCGTTGCTTCGTAAATATTATACATGTATCTT
  TCTTTTTGGAGCATTTTGATTATTACTCTCAGACGTGCGTGGCAACAAGTGACTGAGACC
  TAGAAATCCAAGCGTTGGAGGTCCTGAGGCCAGCCTAAGTCGCTTCAAAATGGAACGAAG
  GCGTTTGCGGGGTTCCATTCAGAGCCGATACATCAGCATGAGTGTGTGGACAAGCCCACG
  GAGACTTGTGGAGCTGGCAGGGCAGAGCCTGCTGAAGGATGAGGCCCTGGCCATTGCCGC
  CCTGGAGTTGCTGCCCAGGGAGCTCTTCCCGCCACTCTTCATGGCAGCCTTTGACGGGAG
  ACACAGCCAGACCCTGAAGGCAATGGTGCAGGCCTGGCCCTTCACCTGCCTCCCTCTGGG
  AGTGCTGATGAAGGGACAACATCTTCACCTGGAGACCTTCAAAGCTGTGCTTGATGGACT
  TGATGTGCTCCTTGCCCAGGAGGTTCGCCCCAGGAGGTGGAAACTTCAAGTGCTGGATTT
  ACGGAAGAACTCTCATCAGGACTTCTGGACTGTATGGTCTGGAAACAGGGCCAGTCTGTA
  CTCATTTCCAGAGCCAGAAGCAGCTCAGCCCATGACAAAGAAGCGAAAAGTAGATGGTTT
  GAGCACAGAGGCAGAGCAGCCCTTCATTCCAGTAGAGGTGCTCGTAGACCTGTTCCTCAA
  GGAAGGTGCCTGTGATGAATTGTTCTCCTACCTCATTGAGAAAGTGAAGCGAAAGAAAAA
  TGTACTACGCCTGTGCTGTAAGAAGCTGAAGATTTTTGCAATGCCCATGCAGGATATCAA
  GATGATCCTGAAAATGGTGCAGCTGGACTCTATTGAAGATTTGGAAGTGACTTGTACCTG
  GAAGCTACCCACCTTGGCGAAATTTTCTCCTTACCTGGGCCAGATGATTAATCTGCGTAG
  ACTCCTCCTCTCCCACATCCATGCATCTTCCTACATTTCCCCGGAGAAGGAAGAGCAGTA
  TATCGCCCAGTTCACCTCTCAGTTCCTCAGTCTGCAGTGCCTGCAGGCTCTCTATGTGGA
  CTCTTTATTTTTCCTTAGAGGCCGCCTGGATCAGTTGCTCAGGCACGTGATGAACCCCTT
  GGAAACCCTCTCAATAACTAACTGCCGGCTTTCGGAAGGGGATGTGATGCATCTGTCCCA
  GAGTCCCAGCGTCAGTCAGCTAAGTGTCCTGAGTCTAAGTGGGGTCATGCTGACCGATGT
  AAGTCCCGAGCCCCTCCAAGCTCTGCTGGAGAGAGCCTCTGCCACCCTCCAGGACCTGGT
  CTTTGATGAGTGTGGGATCACGGATGATCAGCTCCTTGCCCTCCTGCCTTCCCTGAGCCA
  CTGCTCCCAGCTTACAACCTTAAGCTTCTACGGGAATTCCATCTCCATATCTGCCTTGCA
  GAGTCTCCTGCAGCACCTCATCGGGCTGAGCAATCTGACCCACGTGCTGTATCCTGTCCC
  CCTGGAGAGTTATGAGGACATCCATGGTACCCTCCACCTGGAGAGGCTTGCCTATCTGCA
  TGCCAGGCTCAGGGAGTTGCTGTGTGAGTTGGGGCGGCCCAGCATGGTCTGGCTTAGTGC
  CAACCCCTGTCCTCACTGTGGGGACAGAACCTTCTATGACCCGGAGCCCATCCTGTGCCC
  CTGTTTCATGCCTAACTAGCTGGGTGCACATATCAAATGCTTCATTCTGCATACTTGGAC
  ACTAAAGCCAGGATGTGCATGCATCTTGAAGCAACAAAGCAGCCACAGTTTCAGACAAAT
  GTTCAGTGTGAGTGAGGAAAACATGTTCAGTGAGGAAAAAACATTCAGACAAATGTTCAG
  TGAGGAAAAAAAGGGGAAGTTGGGGGTAGGCAGATGTTGACTTGAGGAGTTAATGTGCTC
  TTTGGGGAGATACATCTTATAGAGTTAGAAATAGAATCTGAATTTCTAAAGGGAGATTCT
  GGCTTGGGAAGTACATGTAGGAGTTAATCCCTGTGTAGACTGTTGTAAAGAAACTGTTGA
  AAATAAAGAGAAGCAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_206955.1 unedited
  GGGGCTTTGTATACGACTCCTATAGGGCGGCGCGGCGATCGCGGCGCGCCGGTACCGGTC
  CGGAATTCCCGGGATGCCAGAAGCCGGGCCTGCAGCGCCTCAGCACCGCTCCGGGACACC
  CCACCCGCTTCCCAGGCGTGACCTGTCAACAGCAACTTCGCGGTGTGGTGAACTCTCTGA
  GGAAAAACGTAAGTTCGAGCCCTGATTCCTCCGCTTCCCCGCAGGGTGACCTTGGGCTTG
  TGCCCCCGGCACCACCCCTGTCCCGGGTCCCTGTTTTCTCTCTGGAAATGGGTTGAAGAC
  CAAAGAAAATAATGTGCGCCACTTGGGTCTCTTTTGTTTTCGCCTGCCCCGGAAAATTGG
  CCCCAGTTGAGGAGTTGTGGCTGTAAGGATGCCTTGAACCGAGGCGGCGGTGCTCGTGGT
  TGGAGCTCTCCAGGGTGGGTGCGCATTTGTAATGCGGTGGATGCTCTGGGACTCGGCCCC
  TCTGAAGGTGCTTGGGGGTTGGGGACGGCCCAGGCAGCAGTGGCGTAGGCGTCCTAGGAA
  GGCGGGAGCAGAGGCAGAAATGTCGCTGCAAGACCGTAGTCAGGGTCCTTGACCACAGGG
  GTCACTTGTGACCAACCACATGGTCTGTTGTTCCTCCTGCCCCCTGGTTCAGCCCAGGAA
  ACACTGGTGCTCAGTTTGGAGCCAGAGATTTGCACTGAAAGGGCGGGATTGAGTCGCCAG
  TTGTCAGTTTCCTCAGCAGTATTCGCGGAGTTTTCACAGGAGCCCGTTGCTTCGTAAATA
  TTTATACATGTATCTTTTCTTTTTGAGCATTTTGATTATTACTCTCAGACGTGGCGTGGC
  AACAAGTGACTGAGACCTAGAAATCAAGCGTTGAGTCTTGAGCAGCCTAGTCGCTTCAAA
  TGACGAGCGTTGCGGGTCATTCCAAGCGATCATCAGCATGGAGTGTTGTGCAAGCCACGG
  AACTTGGGGAACCTGGCAAGCG
• Restriction Sites: Please inquire
• ACCN: NM_206955
• ORF Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_206955.1, NP_996838.1
• RefSeq Size: 2220
• RefSeq ORF: 1530
• Locus ID: 23532
• Gene Summary: This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a).