RBPJK (RBPJ) (NM_203284) Human Untagged Clone

CAT#: SC321953

RBPJ (untagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBPJ
• Synonyms: AOS3; CBF1; csl; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJK; RBPSUH; SUH
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC321953 GCGCCTGTTGTGACAGGTCATTGAAATTATGAGACTATCATTCAAATGGAAGCATTATAG
  TTCTTCGGAACCATTATGATCTCAAAACGAAAGGAGAATGATACAGATACACTGGCTGAG
  GTGTTTTGAGGTGCATCGAAGTGTTCCAAGCTGTGACTTACCTTAACATGTTCTTGAAGT
  ACCATGGCGTGGATTAAAAGGAAATTTGGTGAGCGGCCTCCACCTAAACGACTTACTAGG
  GAAGCTATGCGAAATTATTTAAAAGAGCGAGGGGATCAAACAGTACTTATTCTTCATGCA
  AAAGTTGCACAGAAGTCATATGGAAATGAAAAAAGGTTTTTTTGCCCACCTCCTTGTGTA
  TATCTTATGGGCAGTGGATGGAAGAAAAAAAAAGAACAAATGGAACGCGATGGTTGTTCT
  GAACAAGAGTCTCAACCGTGTGCATTTATTGGGATAGGAAATAGTGACCAAGAAATGCAG
  CAGCTAAACTTGGAAGGAAAGAACTATTGCACAGCCAAAACATTGTATATATCTGACTCA
  GACAAGCGAAAGCACTTCATGTTGTCTGTAAAGATGTTCTATGGCAACAGTGATGACATT
  GGTGTGTTCCTCAGCAAGCGGATAAAAGTCATCTCCAAACCTTCCAAAAAGAAGCAGTCA
  TTGAAAAATGCTGACTTATGCATTGCCTCAGGAACAAAGGTGGCTCTGTTTAATCGACTA
  CGATCCCAGACAGTTAGTACCAGATACTTGCATGTAGAAGGAGGTAATTTTCATGCCAGT
  TCACAGCAGTGGGGAGCCTTTTTTATTCATCTCTTGGATGATGATGAATCAGAAGGAGAA
  GAATTCACAGTCCGAGATGGCTACATCCATTATGGACAAACAGTCAAACTTGTGTGCTCA
  GTTACTGGCATGGCACTCCCAAGATTGATAATTAGGAAAGTTGATAAGCAGACCGCATTA
  TTGGATGCAGATGATCCTGTGTCACAACTCCATAAATGTGCATTTTACCTTAAGGATACA
  GAAAGAATGTATTTGTGCCTTTCTCAAGAAAGAATAATTCAATTTCAGGCCACTCCATGT
  CCAAAAGAACCAAATAAAGAGATGATAAATGATGGCGCTTCCTGGACAATCATTAGCACA
  GATAAGGCAGAGTATACATTTTATGAGGGAATGGGCCCTGTCCTTGCCCCAGTCACTCCT
  GTGCCTGTGGTAGAGAGCCTTCAGTTGAATGGCGGTGGGGACGTAGCAATGCTTGAACTT
  ACAGGACAGAATTTCACTCCAAATTTACGAGTGTGGTTTGGGGATGTAGAAGCTGAAACT
  ATGTACAGGTGTGGAGAGAGTATGCTCTGTGTCGTCCCAGACATTTCTGCATTCCGAGAA
  GGTTGGAGATGGGTCCGGCAACCAGTCCAGGTTCCAGTAACTTTGGTCCGAAATGATGGA
  ATCATTTATTCCACCAGCCTTACCTTTACCTACACACCAGAACCAGGGCCGCGGCCACAT
  TGCAGTGCAGCAGGAGCAATCCTTCGAGCCAATTCAAGCCAGGTGCCCCCTAACGAATCA
  AACACAAACAGCGAGGGAAGTTACACAAACGCCAGCACAAATTCAACCAGTGTCACATCA
  TCTACAGCCACAGTGGTATCCTAACTACCGTCTTTTTGCTAGGACTTAAACTGACTTGAG
  TGTGGCAAAAAGTTAACAAAAAAGGAGAAAAAATGAACAATCGTTTGTGGTTTCTTGGGA
  AAACTTTTCATACCAGGTGATACTATTCAAAAACCCCGTTGTCTCCCTGCAAGTGCTGAT
  TTGAAATGCAGAAGCCACAGTAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_203284
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203284.1, NP_976029.1
• RefSeq Size: 2499 bp
• RefSeq ORF: 1461 bp
• Locus ID: 3516
• Cytogenetics: 4p15.2
• Protein Families: Transcription Factors
• Protein Pathways: Notch signaling pathway
• Gene Summary: 'The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.