EIF2B4 (NM_001034116) Human Untagged Clone

CAT#: SC322020

EIF2B4 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF2B4
• Synonyms: EIF-2B; EIF2B; EIF2Bdelta
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322020 CCTAGGACTGAGGGCGATGGCTGCTGTGGCCGTGGCTGTTCGCGAGGACTCGGGATCCGG
  GATGAAGGCGGAGCTTCCCCCTGGGCCTGGGGCAGTGGGGAGGGAAATGACCAAAGAAGA
  AAAGCTGCAGCTTCGGAAGGAAAAGAAACAGCAGAAGAAGAAACGGAAGGAAGAAAAGGG
  GGCAGAACCAGAGACTGGCTCTGCTGTATCTGCAGCCCAATGTCAAGTAGGCCCAACCAG
  AGAACTGCCAGAATCGGGCATTCAGTTGGGCACTCCTCGGGAGAAAGTTCCAGCTGGTCG
  GAGTAAGGCCGAACTTCGGGCTGAGCGTCGAGCCAAGCAGGAGGCCGAGCGGGCCCTGAA
  ACAGGCAAGAAAAGGGGAACAAGGAGGACCACCTCCTAAGGCCAGCCCCAGCACAGCTGG
  AGAAACCCCCTCAGGAGTGAAGCGTCTCCCTGAGTACCCTCAGGTTGATGACCTACTTCT
  GAGAAGGCTTGTTAAAAAACCAGAGCGTCAACAGGTTCCTACACGAAAGGATTATGGATC
  CAAAGTCAGTCTCTTCTCTCACCTACCCCAGTACAGCAGACAAAACTCTCTGACCCAGTT
  TATGAGCATCCCATCCTCTGTGATCCACCCAGCCATGGTGCGACTCGGCCTGCAGTACTC
  CCAGGGCCTGGTCAGTGGCTCCAATGCCCGGTGTATTGCCCTGCTTCGTGCCTTGCAGCA
  GGTGATTCAGGATTACACAACACCGCCTAATGAAGAACTCTCCAGGGATCTAGTGAATAA
  ACTAAAACCCTACATGAGCTTCCTGACTCAGTGCCGTCCCCTGTCAGCGAGCATGCACAA
  CGCCATCAAGTTCCTTAACAAGGAAATCACCAGTGTGGGCAGTTCCAAGCGGGAAGAGGA
  GGCCAAGTCAGAACTTCGAGCAGCCATTGATCGGTATGTGCAAGAGAAGATTGTGCTAGC
  AGCTCAGGCAATTTCACGCTTTGCTTACCAGAAGATCAGTAATGGAGATGTGATCCTGGT
  ATATGGATGCTCATCTCTGGTATCACGAATTCTTCAGGAGGCTTGGACAGAGGGCCGGCG
  GTTTCGGGTGGTAGTGGTGGACAGCCGGCCATGGCTGGAAGGAAGGCACACACTACGTTC
  TCTAGTCCATGCTGGTGTCCCAGCCTCCTACCTGCTGATTCCTGCAGCCTCCTATGTGCT
  CCCAGAGGTTTCCAAGGTGCTATTGGGAGCTCATGCACTCTTGGCCAACGGGTCTGTGAT
  GTCACGGGTAGGGACAGCACAGTTAGCCCTGGTGGCTCGAGCCCATAATGTACCAGTGCT
  GGTTTGCTGTGAAACATACAAGTTCTGTGAGCGTGTGCAGACTGATGCCTTTGTCTCTAA
  TGAGCTAGATGACCCTGATGATCTGCAATGTAAGCGGGGAGAACATGTTGCGCTGGCTAA
  CTGGCAGAACCACGCATCCCTACGGTTGTTGAATCTAGTCTATGATGTGACTCCCCCAGA
  GCTTGTGGATCTGGTGATCACGGAGCTGGGGATGATCCCTTGCAGTTCTGTACCTGTTGT
  TCTACGAGTCAAGAGCAGTGACCAGTGACGGGGGAAACACAGGGTTAATAAATGCCATAC
  TCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001034116
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034116.1, NP_001029288.1
• RefSeq Size: 1763 bp
• RefSeq ORF: 1572 bp
• Locus ID: 8890
• Cytogenetics: 2p23.3
• Gene Summary: Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 4.