RBM3 (NM_006743) Human Untagged Clone

CAT#: SC322227

RBM3 (untagged)-Human RNA binding motif (RNP1, RRM) protein 3 (RBM3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBM3
• Synonyms: IS1-RNPL; RNPL
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322227 GCTCCTCCGAGCTCGCTGTTCGTCCGGGTTTTTTACGTTTTAATTTCCAGGACTTGAACT
  GCCATGTCCTCTGAAGAAGGAAAGCTCTTCGTGGGAGGGCTCAACTTTAACACCGACGAG
  CAGGCACTGGAAGACCACTTCAGCAGTTTCGGACCTATCTCTGAGGTGGTCGTTGTCAAG
  GACCGGGAGACTCAGCGGTCCAGGGGTTTTGGTTTCATCACCTTCACCAACCCAGAGCAT
  GCTTCAGTTGCCATGAGAGCCATGAACGGAGAGTCTCTGGATGGTCGTCAGATCCGTGTG
  GATCATGCAGGCAAGTCTGCTCGGGGAACCAGAGGAGGTGGCTTTGGGGCCCATGGGCGT
  GGTCGCAGCTACTCTAGAGGTGGTGGGGACCAGGGCTATGGGAGTGGCAGGTATTATGAC
  AGTCGACCTGGAGGGTATGGATATGGATATGGACGTTCCAGAGACTATAATGGCAGAAAC
  CAGGGTGGTTATGACCGCTACTCAGGAGGAAATTACAGAGACAATTATGACAACTGAAAT
  GAGACATGCACATAATATAGATACACAAGGAATAATTTCTGATCCAGGATCGTCCTTCCA
  AATGGCTGTATTTATAAAGGTTTTTGGAGCTGCACTGAAGCATCTTATTTTATAGTATAT
  CAACCTTTTGTTTTTAAATTGACCTGCCAAGGTAGCTGAAGACCTTTTAGACAGTTCCAT
  CTTTTTTTTTAAATTTTTTCTGCCTATTTAAAGACAAATTATGGGACGTTTGTAGAACCT
  GAGTATTTTTCTTTTTACCAGTTTTTTAGTTTGAGCTCTTAGGTTTATTGGAGCTAGCAA
  TAATTGGTTCTGGCAAGTTTGGCCAGACTGACTTCAAAAAATTAATGTGTATCCAGGGAC
  ATTTTAAAAACCTGTACACAGTGTTTATTGTGGTTAGGAAGCAATTTCCCAATGTACCTA
  TAAGAAATGTGCATCAAGCCAGCCTGACCAACATGGTGAAACCCCATCTGTACTAAACAT
  AAAAAAATTAGCCTGGCATGGTGGTGTACGCCTGTAATCCCAGTGACTTGGGAGGCTGAG
  GCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTAAGATCGCGCCACTG
  TACTCCAGCCTGGGCAACAGCGAGACTCCATCTCAAAAAAAAAGGAAATGTGTATCAAGA
  ACATGATTATCCAGGGGTATTTTCTAATTCAGATCATCAAACTGATTATATAGAAGAGTT
  GGCTTTAAAATGTTTGCAAATGTCTTTTTTTTTTTAATACTGGAAGAAAAAATATTCTGT
  TGTGTCTCATACAGTGCTTAGGATGTCTTTCACAGAGCTTATTAAAAAGATGAAACCTGA
  AAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006743
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006743.3, NP_006734.1
• RefSeq Size: 1588 bp
• RefSeq ORF: 474 bp
• Locus ID: 5935
• Cytogenetics: Xp11.23
• Domains: RRM
• Gene Summary: 'This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (a).