ARSB (NM_198709) Human Untagged Clone

CAT#: SC322561

ARSB (untagged)-Human arylsulfatase B (ARSB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARSB
• Synonyms: ASB; G4S; MPS6
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322561 CTTGAAAGTAACCGCACCTTCCAAAGGGCACCGTGCAATCAGACTGAAACCACGGTGCAA
  ATTTAATTGCCGGGGAAGATAACGGGCCTTGGTGCCCTCCAAGCGTCAGCTGAGTTTCCA
  AGAAGCCGGGCAGCGGGCGCCCGCGGGTTCGTCTCTGGCTCCTCCTCCGCCACAGCAGCC
  GGGGGCCCGGGTCGGAGGCGGCGGGGGCCGAGCGCCCGGCCTCGCAAGCCCACGGCCCGC
  TGGGGGTGCCGTCCCGCGCCGGGGCGGAGCAGGCCCCGGCAGCCCAGTTCCTCATTCTAT
  CAGCGGTACAAGGGGCTGGTGGCGCCACAGGCGCTGGGACCGCGGGCGGACAAGGATGGG
  TCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTCCCCGT
  CGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGGGCCAG
  CCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTCGGCTT
  CCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTGCTCCT
  GGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACTGGCCG
  CTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGCTGTGT
  TCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACCCATAT
  GGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGAGGATT
  TGATACCTACTTTGGATATCTCCTGGGTAGTGAAGATTATTATTCCCATGAACGCTGTAC
  ATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAAGAAGT
  TGCAACAGGATATAAAAATATGTATTCAACAAACATATTCACCAAAAGGGCTATAGCCCT
  CATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCTGTGCA
  TGAGCCCCTTCAGGTCCCTGAGGAATACTTGAAGCCATATGACTTTATCCAAGACAAGAA
  CAGGCATCACTATGCAGGAATGGTGTCCCTTATGGATGAAGCAGTAGGAAATGTCACTGC
  AGCTTTAAAAAGCAGTGGGCTCTGGAACAACACGGTGTTCATCTTTTCTACAGATAACGG
  AGGGCAGACTTTGGCAGGGGGTAATAACTGGCCCCTTCGAGGAAGAAAATGGAGCCTGTG
  GGAAGGAGGCGTCCGAGGGGTGGGCTTTGTGGCAAGCCCCTTGCTGAAGCAGAAGGGCGT
  GAAGAACCGGGAGCTCATCCACATCTCTGACTGGCTGCCAACACTCGTGAAGCTGGCCAG
  GGGACACACCAATGGCACAAAGCCTCTGGATGGCTTCGACGTGTGGAAAACCATCAGTGA
  AGGAAGCCCATCCCCCAGAATTGAGCTGCTGCATAATATTGACCCGAACTTCGTGGACTC
  TTCACCGTACTGGCCTGAGTGCTCGCTGCTGTTGTAGCTACCACCAACTTTCTACTGAAG
  ATGATAACCCAGGGCATAAGAAATGACTTCAGACCCAAGGTTCTGAAAGGGCCCCTCAAG
  GCCTCGGGTGGCTCCTGCAGAAGTGGCAGAAGAGGCGGGAACTAGGAACCTGGCATCATA
  GGAAAAGTGCCTTCTCCAAGAAAGAAGGGGCCCCAAGAGGCTGTCTTACTTAGATCAACC
  ATAAACTACCACAGATGGGTCATTTCTTATACTATTTCAAAATATCTTTGAAGATGAGAA
  TTCATTTGTGTCCTTCATAGACCAAAGTTCTTTGTGTTACCTTTTCCCAAAAGTAAATTC
  CTTTCCCTTTATTCATTCCTTGTGGAAATAAAATGCAAGCCCTTTAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_198709
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198709.1, NP_942002.1
• RefSeq Size: 1983 bp
• RefSeq ORF: 1242 bp
• Locus ID: 411
• Cytogenetics: 5q14.1
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: 'Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (2) has an alternate 3' sequence, as compared to variant 1. The resulting isoform 2 has a distinct and shorter C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments.