BMAL1 (ARNTL) (NM_001178) Human Untagged Clone

CAT#: SC322615

ARNTL (untagged)-Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARNTL
• Synonyms: bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322615 TTAGGGAAGGCAGAAAGTAGGTCAGGGACGGAGGTGCCTGTTTACCCGCGCCGGACTCAC
  CGCCGCCGCCGCCGCGGGATCCGAGTGCGGGCGCGGGCGCGGGCGCTCCCGGCGAGCCAC
  GGTGGTGCTGGCTAGAGTGTATACGTTTGGACCCAAGCTTAACTTTTCCAATGTGGAATC
  CTGGGCCTTCATTGGTTCCGATGTCATAGGAATCTACGCTAGAGGGCTTCCTGTGTGCCG
  GGCACTGTGGTTTTTGGAAGAAGCTGACCGCCTGAAAAGAAATTATAAAACATGAAAATC
  GCTTTGAGGTGACCAAGTCCAGAGGCCCCTAACTCCTCCCAAGCTGGATCTGGGGTGTAA
  GAACTGTGACTTCAGATCATCCAATGGCAGACCAGAGAATGGACATTTCTTCAACCATCA
  GTGATTTCATGTCCCCGGGCCCCACCGACCTGCTTTCCAGCTCTCTTGGTACCAGTGGTG
  TGGATTGCAACCGCAAACGGAAAGGCAGCTCCACTGACTACCAAGAAAGCATGGACACAG
  ACAAAGATGACCCTCATGGAAGGTTAGAATATACAGAACACCAAGGAAGGATAAAAAATG
  CAAGGGAAGCTCACAGTCAGATTGAAAAGCGGCGTCGGGATAAAATGAACAGTTTTATAG
  ATGAATTGGCTTCTTTGGTACCAACATGCAACGCAATGTCCAGGAAATTAGATAAACTTA
  CTGTGCTAAGGATGGCTGTTCAGCACATGAAAACATTAAGAGGTGCCACCAATCCATACA
  CAGAAGCAAACTACAAACCAACTTTTCTATCAGACGATGAATTGAAACACCTCATTCTCA
  GGGCAGCAGATGGATTTTTGTTTGTCGTAGGATGTGACCGAGGGAAGATACTCTTTGTCT
  CAGAGTCTGTCTTCAAGATCCTCAACTACAGCCAGAATGATCTGATTGGTCAGAGTTTGT
  TTGACTACCTGCATCCTAAAGATATTGCCAAAGTCAAGGAGCAGCTCTCCTCCTCTGACA
  CCGCACCCCGGGAGCGGCTCATAGATGCAAAAACTGGACTTCCAGTTAAAACAGATATAA
  CCCCTGGGCCATCTCGATTATGTTCTGGAGCACGACGTTCTTTCTTCTGTAGGATGAAGT
  GTAACAGGCCTTCAGTAAAGGTTGAAGACAAGGACTTCCCCTCTACCTGCTCAAAGAAAA
  AAGATCGAAAAAGCTTCTGCACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCA
  CAAAGATGGGGCTGGATGAAGACAACGAACCAGACAATGAGGGGTGTAACCTCAGCTGCC
  TCGTCGCAATTGGACGACTGCATTCTCATGTAGTTCCACAACCAGTGAACGGGGAAATCA
  GGGTGAAATCTATGGAATATGTTTCTCGGCACGCGATAGATGGAAAGTTTGTTTTTGTAG
  ACCAGAGGGCAACAGCTATTTTGGCATATTTACCACAAGAACTTCTAGGCACATCGTGTT
  ATGAATATTTTCACCAAGATGACATAGGACATCTTGCAGAATGTCATAGGCAAGTTTTAC
  AGACGAGAGAAAAAATTACAACTAATTGCTATAAATTTAAAATCAAAGATGGTTCTTTTA
  TCACACTACGGAGTCGATGGTTCAGTTTCATGAACCCTTGGACCAAGGAAGTAGAATATA
  TTGTCTCAACTAACACTGTTGTTTTAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCC
  CACAGCTCACAGCATCCCCCCACAGCATGGACAGCATGCTGCCCTCTGGAGAAGGTGGCC
  CAAAGAGGACCCACCCCACTGTTCCAGGGATTCCAGGGGGAACCCGGGCTGGGGCAGGAA
  AAATAGGCCGAATGATTGCTGAGGAAATCATGGAAATCCACAGGATAAGAGGGTCATCGC
  CTTCTAGCTGTGGCTCCAGCCCATTGAACATCACGAGTACGCCTCCCCCTGATGCCTCTT
  CTCCAGGAGGCAAGAAGATTTTAAATGGAGGGACTCCAGACATTCCTTCCAGTGGCCTAC
  TATCAGGCCAGGCTCAGGAGAACCCAGGTTATCCATATTCTGATAGTTCTTCTATTCTTG
  GTGAGAACCCCCACATAGGTATAGACATGATTGACAACGACCAAGGATCAAGTAGTCCCA
  GTAATGATGAGGCAGCAATGGCTGTCATCATGAGCCTCTTGGAAGCAGATGCTGGACTGG
  GTGGCCCTGTTGACTTTAGTGACTTGCCATGGCCGCTGTAAACACTACATGTTGCTTTGG
  CAACAGCTATAGTATCAAAGTGCATTACTGGTGGAGTTTTACAGTCTGTGAAGCTTACTG
  GATAAGGAGAGAATAGCTTTTATGTACTGACTTCATAAAAGCCATCTCAGAGCCATTGAT
  ACAAGTCAATCTTACTATATGTAACTTCAGACAAAGTGGAACTAAGCCTGCTCCAGTGTT
  TCCTCATCATTGATTATTGGGCTAGCTGTGGATAGCTTGCATTAATTGTATATTTTGGAT
  TCTGTTTGTGTTGAATTTTTTAATCATTGTGCACAGAAGCATCATTGGTAGCTTTTATAT
  GCAAATGGTCATTTCAGATGTATGGTGTTTTTACACTACAAAGAAGTCCCCCATGTGGAT
  ATTTCTTATACTAATTGTATCATAAAGCCGTTTATTCTTCCTTGTAAGAATCCTTTACTA
  TAAATATGGGTTAAAGTATAATGTACTAGACAGTTAAATATTTTTAATAAATGTTTCCCT
  TGTTCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAA
• Restriction Sites: Please inquire
• ACCN: NM_001178
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178.4, NP_001169.3
• RefSeq Size: 2812 bp
• RefSeq ORF: 1878 bp
• Locus ID: 406
• Cytogenetics: 11p15.3
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: 'The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (1) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 4. The resulting isoform (a) is 1 aa shorter compared to isoform c. Variants 1, 2, 10, and 11 all encode isoform a.