RAD21 (NM_006265) Human Untagged Clone

CAT#: SC322657

RAD21 (untagged)-Human RAD21 homolog (S. pombe) (RAD21)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD21
• Synonyms: CDLS4; hHR21; HR21; HRAD21; MCD1; MGS; NXP1; SCC1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322657 CACCTCGCAAGGGCTCCCCCCTTTCCCCACCCCCTCCCCCGACCCTTTTCCCCTCCCCGG
  GCCACCCAGCCCGCCCAACTCCCAGCGGAGAGCAAGGTTTTCTTCTGTTTTCATAGCCAG
  CCAGAACAATGTTCTACGCACATTTTGTTCTCAGTAAAAGAGGGCCTCTGGCCAAAATTT
  GGCTAGCGGCCCATTGGGATAAGAAGCTAACCAAAGCCCATGTGTTCGAGTGTAATTTAG
  AGAGCAGCGTGGAGAGTATCATCTCACCAAAGGTGAAAATGGCATTACGGACATCAGGAC
  ATCTCTTACTGGGAGTAGTTCGAATCTATCACAGGAAAGCCAAATACCTTCTTGCAGACT
  GTAATGAAGCATTCATTAAGATAAAGATGGCTTTTCGGCCAGGTGTGGTTGACCTGCCTG
  AGGAAAATCGGGAAGCAGCTTATAATGCCATTACTTTACCTGAAGAATTTCATGACTTTG
  ATCAGCCACTGCCTGACTTAGATGACATCGATGTGGCCCAGCAGTTCAGCTTGAATCAGA
  GTAGAGTGGAAGAGATAACCATGAGAGAAGAAGTTGGGAACATCAGTATTTTACAAGAAA
  ATGATTTTGGTGATTTTGGAATGGATGATCGTGAGATAATGAGAGAAGGCAGTGCTTTTG
  AGGATGACGACATGTTAGTAAGCACTACTACTTCTAACCTCCTATTAGAGTCTGAACAGA
  GCACCAGCAATCTGAATGAGAAAATTAACCATTTAGAATATGAAGATCAATATAAGGATG
  ATAATTTTGGAGAAGGAAATGATGGTGGAATATTAGATGACAAACTTATTAGTAATAATG
  ATGGCGGTATCTTTGATGATCCCCCTGCCCTCTCTGAGGCAGGGGTGATGTTGCCAGAGC
  AGCCTGCACATGACGATATGGATGAGGATGATAATGTATCAATGGGTGGGCCTGATAGTC
  CTGATTCAGTGGATCCCGTTGAACCAATGCCAACCATGACTGATCAAACAACACTTGTTC
  CAAATGAGGAAGAAGCATTTGCATTGGAGCCTATTGATATAACTGTTAAAGAAACAAAAG
  CCAAGAGGAAGAGGAAGCTAATTGTTGACAGTGTCAAAGAGTTGGATAGCAAGACAATTA
  GAGCCCAACTTAGTGATTATTCAGATATTGTTACTACTTTGGATCTGGCACCGCCCACCA
  AGAAATTGATGATGTGGAAAGAGACAGGAGGAGTAGAAAAACTGTTTTCTTTACCTGCTC
  AGCCTTTGTGGAATAACAGACTACTGAAGCTCTTTACACGCTGTCTTACACCGCTTGTAC
  CAGAAGACCTTAGAAAAAGGAGGAAAGGAGGAGAGGCAGATAATTTGGATGAATTCCTCA
  AAGAATTTGAAAATCCAGAGGTTCCTAGAGAGGACCAGCAACAGCAGCATCAGCAGCGTG
  ATGTTATCGATGAGCCCATTATTGAAGAGCCAAGCCGCCTCCAGGAGTCAGTGATGGAGG
  CCAGCAGAACAAACATAGATGAGTCAGCTATGCCTCCACCACCACCTCAGGGAGTTAAGC
  GAAAAGCTGGACAAATTGACCCAGAGCCTGTGATGCCTCCTCAGCAGGTAGAGCAGATGG
  AAATACCACCTGTAGAGCTTCCCCCAGAAGAACCTCCAAATATCTGTCAGCTAATACCAG
  AGTTAGAACTTCTGCCAGAAAAAGAGAAGGAGAAAGAGAAGGAAAAAGAAGATGATGAAG
  AGGAAGAGGATGAAGATGCATCAGGGGGCGATCAAGATCAGGAAGAAAGAAGATGGAACA
  AAAGGACTCAGCAGATGCTTCATGGTCTTCAGCGTGCTCTTGCTAAAACTGGAGCTGAAT
  CTATCAGTTTGCTTGAGTTATGTCGAAATACGAACAGAAAACAAGCTGCCGCAAAGTTCT
  ACAGCTTCTTGGTTCTTAAAAAGCAGCAAGCTATTGAGCTGACACAGGAAGAACCGTACA
  GTGACATCATCGCAACACCTGGACCAAGGTTCCATATTATATAAGGAGCTAGAAGCATTA
  TAGCTAGTGTTTGATTCACTAGTGCTTACAAATTGCCCCCATGTGTAGGGGACACAGAAC
  CCTTTGAGAAAACTTAGATTTTTGTCTGTACAAAGTCTTTGCCTTTTTCCTTCTTCATTT
  TTTTCCAGTACATTAAATTTGTCAATTTCATCTTTGAGGGAAACTGATTAGATGGGTTGT
  GTTTGTGTTCTGATGGAGAAAACAGCACCCCAAGGACTCAGAAGATGATTTTAACAGTTC
  AGAACAGATGTGTGCAATATTGGTGCATGTAATAATGTTGAGTGGCAGTCAAAAGTCATG
  ATTTTTATCTTAGTTCTTCATTACTGCATTGAAAAGGAAAACCTGTCTGAGAAAATGCCT
  GACAGTTTAATTTAAAACTATGGTGTAAGTCTTTGACAAGAAAAAAAAACAAACAAACAC
  TTCTTTCCATCAGTAACACTGGCAATCTTCCTGTTAACCACTCTCCTTAGGGATGGTATC
  TGAAACAACAATGGTCACCCTCTTGAGATTCGTTTTAAGTGTAATTCCATAATGAGCAGA
  GGTGTACGCGAAATTGTGTTATGACTGATAGCCTTCAGCTACAAAAAGATAGGACTGACC
  TGGTTTAAAGTGTTCTATTTTGTAAATCATTCCATTTGAGTCTTTCTGATGAACTTGGCT
  ATACTGAAATCTGTTATTTTAGTGAGGCTCCAAAATGAGCAAAGCTAGGCCTGATTAGAG
  TAGAGTGACTATTAAAAAACATAACTTTCTAGGAGCTATAAATCAAAGTTTTAAAAAGAT
  GTTTGGATATATTTGAGTATTCCGATCATGAAAACAGAAATTGCCCTGCCTACTACAAGG
  ACAGACTGATGGGAAATTATGCACCTGGTCAACTTAGCTTTTAAGCAGACGATGCTGTAA
  AAACTAACGGCTTCTCTGATATTTATTGTAAGTTTTAGTACTGATCTCCTTTTCCAGTGC
  TGCACACTCCTGGTTTGGAACTTTAATAGCGTTGCAACGAAATCCTATATCCAGTTTCCT
  GTAATTTAATTGAAGAAAAATACATCCAAATAAAGACTTTATTATTAACAGACCAGATAG
  CATCAGAAATCATGTGACTGTTATGATTATCAGAATGTCTTAACTTTTTAGGGCAAAGTT
  AACACTGAAAGTTCTAGCTTAAGTGTTGAAACTTTTGTGGGAAAAAAAAATCACTTTTGA
  AACTCAGACTTCAGTGTATACCCAATAATTTAAAATTATGTGAAATGTTTTAAATTTGTG
  AACTCGTAATTACTGTTTTAATGATTCAGTTTCTTCAGAGTGGTAATTGTATAAAATTGC
  TATTGCAGCTTTACATTCAATATGATGTGCCTGTAAACCAAGGAGTTTTCCCCGTTTGTA
  AAAAGACATTGTAGATAATTGAATGTTTGATTTTAGAAAGGTCATTAGTTTCTTGTTACA
  CATTTTGTTAGTCTGGTTTTTGTTGCTTATCGGGTTTAATATTGTTCTTGAAAATAGTTG
  ATGCTATGTTATGTATAACTTTTCTAATAAAAGTTGTGTTATAAGCTGTAAAAAAAAAAA
  AAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006265
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006265.1, NP_006256.1
• RefSeq Size: 3647 bp
• RefSeq ORF: 1896 bp
• Locus ID: 5885
• Cytogenetics: 8q24.11
• Domains: Rad21_Rec8, Rad21_Rec8_N
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle
• Gene Summary: 'The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]'