RAD21 (NM_006265) Human Untagged Clone

CAT#: SC322657

RAD21 (untagged)-Human RAD21 homolog (S. pombe) (RAD21)


  "NM_006265" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RAD21
Synonyms CDLS4; hHR21; HR21; HRAD21; MCD1; MGS; NXP1; SCC1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for SC322657 CACCTCGCAAGGGCTCCCCCCTTTCCCCACCCCCTCCCCCGACCCTTTTCCCCTCCCCGG
GCCACCCAGCCCGCCCAACTCCCAGCGGAGAGCAAGGTTTTCTTCTGTTTTCATAGCCAG
CCAGAACAATGTTCTACGCACATTTTGTTCTCAGTAAAAGAGGGCCTCTGGCCAAAATTT
GGCTAGCGGCCCATTGGGATAAGAAGCTAACCAAAGCCCATGTGTTCGAGTGTAATTTAG
AGAGCAGCGTGGAGAGTATCATCTCACCAAAGGTGAAAATGGCATTACGGACATCAGGAC
ATCTCTTACTGGGAGTAGTTCGAATCTATCACAGGAAAGCCAAATACCTTCTTGCAGACT
GTAATGAAGCATTCATTAAGATAAAGATGGCTTTTCGGCCAGGTGTGGTTGACCTGCCTG
AGGAAAATCGGGAAGCAGCTTATAATGCCATTACTTTACCTGAAGAATTTCATGACTTTG
ATCAGCCACTGCCTGACTTAGATGACATCGATGTGGCCCAGCAGTTCAGCTTGAATCAGA
GTAGAGTGGAAGAGATAACCATGAGAGAAGAAGTTGGGAACATCAGTATTTTACAAGAAA
ATGATTTTGGTGATTTTGGAATGGATGATCGTGAGATAATGAGAGAAGGCAGTGCTTTTG
AGGATGACGACATGTTAGTAAGCACTACTACTTCTAACCTCCTATTAGAGTCTGAACAGA
GCACCAGCAATCTGAATGAGAAAATTAACCATTTAGAATATGAAGATCAATATAAGGATG
ATAATTTTGGAGAAGGAAATGATGGTGGAATATTAGATGACAAACTTATTAGTAATAATG
ATGGCGGTATCTTTGATGATCCCCCTGCCCTCTCTGAGGCAGGGGTGATGTTGCCAGAGC
AGCCTGCACATGACGATATGGATGAGGATGATAATGTATCAATGGGTGGGCCTGATAGTC
CTGATTCAGTGGATCCCGTTGAACCAATGCCAACCATGACTGATCAAACAACACTTGTTC
CAAATGAGGAAGAAGCATTTGCATTGGAGCCTATTGATATAACTGTTAAAGAAACAAAAG
CCAAGAGGAAGAGGAAGCTAATTGTTGACAGTGTCAAAGAGTTGGATAGCAAGACAATTA
GAGCCCAACTTAGTGATTATTCAGATATTGTTACTACTTTGGATCTGGCACCGCCCACCA
AGAAATTGATGATGTGGAAAGAGACAGGAGGAGTAGAAAAACTGTTTTCTTTACCTGCTC
AGCCTTTGTGGAATAACAGACTACTGAAGCTCTTTACACGCTGTCTTACACCGCTTGTAC
CAGAAGACCTTAGAAAAAGGAGGAAAGGAGGAGAGGCAGATAATTTGGATGAATTCCTCA
AAGAATTTGAAAATCCAGAGGTTCCTAGAGAGGACCAGCAACAGCAGCATCAGCAGCGTG
ATGTTATCGATGAGCCCATTATTGAAGAGCCAAGCCGCCTCCAGGAGTCAGTGATGGAGG
CCAGCAGAACAAACATAGATGAGTCAGCTATGCCTCCACCACCACCTCAGGGAGTTAAGC
GAAAAGCTGGACAAATTGACCCAGAGCCTGTGATGCCTCCTCAGCAGGTAGAGCAGATGG
AAATACCACCTGTAGAGCTTCCCCCAGAAGAACCTCCAAATATCTGTCAGCTAATACCAG
AGTTAGAACTTCTGCCAGAAAAAGAGAAGGAGAAAGAGAAGGAAAAAGAAGATGATGAAG
AGGAAGAGGATGAAGATGCATCAGGGGGCGATCAAGATCAGGAAGAAAGAAGATGGAACA
AAAGGACTCAGCAGATGCTTCATGGTCTTCAGCGTGCTCTTGCTAAAACTGGAGCTGAAT
CTATCAGTTTGCTTGAGTTATGTCGAAATACGAACAGAAAACAAGCTGCCGCAAAGTTCT
ACAGCTTCTTGGTTCTTAAAAAGCAGCAAGCTATTGAGCTGACACAGGAAGAACCGTACA
GTGACATCATCGCAACACCTGGACCAAGGTTCCATATTATATAAGGAGCTAGAAGCATTA
TAGCTAGTGTTTGATTCACTAGTGCTTACAAATTGCCCCCATGTGTAGGGGACACAGAAC
CCTTTGAGAAAACTTAGATTTTTGTCTGTACAAAGTCTTTGCCTTTTTCCTTCTTCATTT
TTTTCCAGTACATTAAATTTGTCAATTTCATCTTTGAGGGAAACTGATTAGATGGGTTGT
GTTTGTGTTCTGATGGAGAAAACAGCACCCCAAGGACTCAGAAGATGATTTTAACAGTTC
AGAACAGATGTGTGCAATATTGGTGCATGTAATAATGTTGAGTGGCAGTCAAAAGTCATG
ATTTTTATCTTAGTTCTTCATTACTGCATTGAAAAGGAAAACCTGTCTGAGAAAATGCCT
GACAGTTTAATTTAAAACTATGGTGTAAGTCTTTGACAAGAAAAAAAAACAAACAAACAC
TTCTTTCCATCAGTAACACTGGCAATCTTCCTGTTAACCACTCTCCTTAGGGATGGTATC
TGAAACAACAATGGTCACCCTCTTGAGATTCGTTTTAAGTGTAATTCCATAATGAGCAGA
GGTGTACGCGAAATTGTGTTATGACTGATAGCCTTCAGCTACAAAAAGATAGGACTGACC
TGGTTTAAAGTGTTCTATTTTGTAAATCATTCCATTTGAGTCTTTCTGATGAACTTGGCT
ATACTGAAATCTGTTATTTTAGTGAGGCTCCAAAATGAGCAAAGCTAGGCCTGATTAGAG
TAGAGTGACTATTAAAAAACATAACTTTCTAGGAGCTATAAATCAAAGTTTTAAAAAGAT
GTTTGGATATATTTGAGTATTCCGATCATGAAAACAGAAATTGCCCTGCCTACTACAAGG
ACAGACTGATGGGAAATTATGCACCTGGTCAACTTAGCTTTTAAGCAGACGATGCTGTAA
AAACTAACGGCTTCTCTGATATTTATTGTAAGTTTTAGTACTGATCTCCTTTTCCAGTGC
TGCACACTCCTGGTTTGGAACTTTAATAGCGTTGCAACGAAATCCTATATCCAGTTTCCT
GTAATTTAATTGAAGAAAAATACATCCAAATAAAGACTTTATTATTAACAGACCAGATAG
CATCAGAAATCATGTGACTGTTATGATTATCAGAATGTCTTAACTTTTTAGGGCAAAGTT
AACACTGAAAGTTCTAGCTTAAGTGTTGAAACTTTTGTGGGAAAAAAAAATCACTTTTGA
AACTCAGACTTCAGTGTATACCCAATAATTTAAAATTATGTGAAATGTTTTAAATTTGTG
AACTCGTAATTACTGTTTTAATGATTCAGTTTCTTCAGAGTGGTAATTGTATAAAATTGC
TATTGCAGCTTTACATTCAATATGATGTGCCTGTAAACCAAGGAGTTTTCCCCGTTTGTA
AAAAGACATTGTAGATAATTGAATGTTTGATTTTAGAAAGGTCATTAGTTTCTTGTTACA
CATTTTGTTAGTCTGGTTTTTGTTGCTTATCGGGTTTAATATTGTTCTTGAAAATAGTTG
ATGCTATGTTATGTATAACTTTTCTAATAAAAGTTGTGTTATAAGCTGTAAAAAAAAAAA
AAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_006265
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006265.1, NP_006256.1
RefSeq Size 3647 bp
RefSeq ORF 1896 bp
Locus ID 5885
Cytogenetics 8q24.11
Domains Rad21_Rec8, Rad21_Rec8_N
Protein Families Druggable Genome
Protein Pathways Cell cycle
Gene Summary 'The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.