DNase II (DNASE2) (NM_001375) Human Untagged Clone

CAT#: SC322699

DNASE2 (untagged)-Human deoxyribonuclease II, lysosomal (DNASE2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNASE2
• Synonyms: DNASE2A; DNL; DNL2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322699 GAAAGGCGCCAGGTGCCAGTCCTGGCCTCTGATGTAACCCAGCGCCCCGCAGTCCCGACA
  CAGATTCCTGGATCTCAGCCCCATAGCAGCTATGATCCCGCTGCTGCTGGCAGCGCTGCT
  GTGCGTCCCCGCCGGGGCCCTGACCTGCTACGGGGACTCCGGGCAGCCTGTAGACTGGTT
  CGTGGTCTACAAGCTGCCAGCTCTTAGAGGGTCCGGGGAGGCGGCGCAGAGAGGGCTGCA
  GTACAAGTATCTGGACGAGAGCTCCGGAGGCTGGCGGGACGGCAGGGCACTCATCAACAG
  CCCGGAGGGGGCCGTGGGCCGAAGCCTGCAGCCGCTGTACCGGAGCAACACCAGCCAGCT
  CGCCTTCCTGCTCTACAATGACCAACCGCCTCAACCCAGCAAGGCTCAGGACTCTTCCAT
  GCGTGGGCACACGAAGGGTGTCCTGCTCCTTGACCACGATGGGGGCTTCTGGCTGGTCCA
  CAGTGTACCTAACTTCCCTCCACCGGCCTCCTCTGCTGCATACAGCTGGCCTCATAGCGC
  CTGTACCTACGGGCAGACCCTGCTCTGTGTGTCTTTTCCCTTCGCTCAGTTCTCGAAGAT
  GGGCAAGCAGCTGACCTACACCTACCCCTGGGTCTATAACTACCAGCTGGAAGGGATCTT
  TGCCCAGGAATTCCCCGACTTGGAGAATGTGGTCAAGGGCCACCACGTTAGCCAAGAACC
  CTGGAACAGCAGCATCACACTCACATCCCAGGCCGGGGCTGTTTTCCAGAGCTTTGCCAA
  GTTCAGCAAATTTGGAGATGACCTGTACTCCGGCTGGTTGGCAGCAGCCCTTGGTACCAA
  CCTGCAGGTCCAGTTCTGGCACAAAACTGTAGGCATCCTGCCCTCTAACTGCTCGGATAT
  CTGGCAGGTTCTGAATGTGAACCAGATAGCTTTCCCTGGACCAGCCGGCCCAAGCTTCAA
  CAGCACAGAGGACCACTCCAAATGGTGCGTGTCCCCAAAAGGGCCCTGGACCTGCGTGGG
  TGACATGAATCGGAACCAGGGAGAGGAGCAACGGGGTGGGGGCACACTGTGTGCCCAGCT
  GCCAGCCCTCTGGAAAGCCTTCCAGCCGCTGGTGAAGAACTACCAGCCCTGTAATGGCAT
  GGCCAGGAAGCCCAGCAGAGCTTATAAGATCTAACCCTTATGGCCAGGTGCAGTGGCTCA
  CGTATGTAATCCCAGCACTTTGGGAAGCCAAGGAGGGAGGATCACTTGAACTCAGGAATT
  CGAGACCAGCCTGGGCTACATAGTGAGACCACATCTCTACTAGAACTTAAAAAAAGTTAG
  CCAGGCACGGTGATAAATGCCTGTAGTCCCAGCCACTGAAGCCAGAGGATCGATTGAACC
  AGGGAGATCATGGTCACAGTGAACTATGATTACGCCAACCTGGGTCACATAGCAAGACTC
  TGTTTCAAAAAAAAAGGGGGGGCGGGGGACGGGTGGGTGCAGTGGCTCACATCTGTAACC
  CCAGCACTTTGGGAGGCTGAGATGGGCAGATCACTTGAGGTCAGGAGTTCGAGACCAGCC
  TGGCCAACATGGTGAAACCCCATATCCATTAAAAATATTTAAAAATTAGCCAGACATGGT
  GGCACGCGTCTGTGGTCCTAGCTCCTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACTCG
  GGAGGCAGAGGTTGTCATGAGCTGAGCTAACACCACGGCACTTCAGCCTGGGTGACAGAA
  TGAGACTCTGTGTCAAAAAAATAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001375
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001375.2, NP_001366.1
• RefSeq Size: 2011 bp
• RefSeq ORF: 1083 bp
• Locus ID: 1777
• Cytogenetics: 19p13.13
• Domains: DNase_II
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• Gene Summary: 'This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]'