COX15 (NM_078470) Human Untagged Clone

CAT#: SC322747

COX15 (untagged)-Human COX15 homolog, cytochrome c oxidase assembly protein (yeast) (COX15), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COX15
• Synonyms: CEMCOX2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322747 GAAGAGGTGGCTGTTCCCTGTCATCAGTATGCAGCGATTGCTCTTTCCGCCGTTGAGGGC
  CTTGAAGGGGAGGCAGTATCTGCCGCTCCTGGCTCCTAGGGCAGCGCCTAGAGCACAGTG
  TGATTGCATCAGGCGCCCTTTGAGGCCAGGGCAATACAGCACCATCTCTGAAGTAGCTTT
  GCAATCTGGAAGGGGTACAGTGTCCCTTCCCTCAAAGGCTGCTGAGCGGGTGGTGGGCCG
  ATGGCTCCTGGTCTGCAGTGGAACAGTGGCTGGAGCAGTTATTCTTGGTGGAGTAACTAG
  GTTGACAGAGTCTGGCCTCTCGATGGTAGATTGGCATTTAATAAAGGAGATGAAGCCACC
  TACAAGCCAAGAGGAATGGGAAGCAGAATTCCAAAGATACCAGCAATTTCCAGAATTTAA
  AATCTTGAATCATGATATGACACTGACAGAATTCAAGTTCATCTGGTACATGGAGTACTC
  ACACCGAATGTGGGGTCGCCTTGTAGGCCTTGTGTACATCCTGCCTGCTGCCTACTTTTG
  GAGAAAGGGCTGGCTCAGCCGTGGCATGAAAGGACGTGTTCTTGCCCTCTGTGGCCTCGT
  CTGCTTCCAGGGTCTGTTGGGATGGTATATGGTGAAAAGTGGACTAGAAGAAAAATCAGA
  CTCCCATGACATCCCTCGGGTCAGTCAGTACCGCCTTGCTGCCCACCTGGGATCAGCCCT
  GGTTCTTTATTGTGCCAGCTTGTGGACCTCACTGTCACTGCTACTCCCTCCGCACAAGTT
  GCCTGAAACCCACCAACTCCTACAGTTGAGACGATTTGCTCATGGAACAGCAGGTCTGGT
  GTTCCTTACGGCCCTCTCAGGGGCTTTTGTGGCAGGGCTAGATGCTGGGCTTGTTTATAA
  CTCCTTTCCCAAAATGGGAGAATCCTGGATCCCGGAGGACCTCTTTACCTTCTCCCCCAT
  CCTGAGGAATGTTTTTGAGAATCCCACCATGGTGCAGTTTGATCACCGGATTCTGGGAAT
  CACTTCAGTCACTGCCATTACAGTGCTCTACTTCCTCTCTCGGAGAATTCCCCTTCCTAG
  AAGGACCAAGATGGCAGCAGTGACTCTGCTGGCTTTGGCGTATACACAGGTGGGCTTGGG
  CATCAGCACGCTGCTGATGTATGTCCCAACTCCTCTGGCCGCCACTCACCAGTCAGGCTC
  CTTGGCTTTGCTCACTGGTGCTCTTTGGCTGATGAATGAACTCCGAAGAGTCCCAAAATG
  ATTCTTAGAGGACCAGCCTCCTGGGACTGTGACTGCTTTTGAGAGCTCTTCAGAGATCAT
  AAGAACTTGGGCTTTTCTACGAGATGACCTTGACATACCAAGTGGTTTCCAAATGGTCAA
  CTTACTTAAAAATCTTTTCCTGTTTTGAGATAGTCACTGGATCAAGAATGCATTAAGTGT
  GGTTACCCTAAATGTTCCCTTTTAAATCTGCTTTTCATGTTGAAAATCAGTTTTAATGTA
  GAGAAAGAAATGTCTGCCATTTGCTGCTTAACAGGCTTTGTGTCAGGTTTTTCAGTGTTG
  GCAAGCTCTTGGTTCTACGTGGATGATTTCTACGTGGATGTTCTCCTGAGTCCTTAATTC
  TGCCTAAATAGAATTTCTTTATGATCTTAACTTCACTTCCATTAGGTGAAGATTGAAAGG
  ATAGGATTGACATACCCAAAGTAGCCAGCTGGTCTTCAGCAAAAAAAAAAAAGCTAATGT
  CTTCTAATATCCTGATTTTCAGAAATGGGGAAATTGCAGATTTTGACAAGCTTAATGTGT
  ATATGTAGCAAAATGGTTTTTAAGTACTTGGAAAAGGAGGTAATCGCCAAATAGTTCCAT
  TTTTTTTTTTTTTTTTTGAGACAGAGTTTCACTCTTGTTGCCCAGGCTGGAGTACAATGG
  CGTGATCTCAGCTCACTCTGCAACCTCCACTTCCCAGGTTCAAGCGATTCTCATGCCTCA
  GCCTCCCGAGTAGCTGGGACTACAGGCACAAGTCACCATGCCTGGCTAATTTTGTATTTT
  TAGTAGAGAAGGGGTTTCACCACATTGGCCGGGCTGGTCTTGAACTCCTGACCTCAGGTA
  ATCCGCCCATCTCGGCTTCCCAAAGTGCTGAGATTACAGGCATGGGCCACCACGCCCAGC
  TAGTTCCTCTTTTGATAAGGCTGTTAACATTACAAGGTCAGAGAGAGGAATGGAATCGAC
  AGTATAAATTGGTTCCTGAGAGGGTTTCCTAACAGCTTTGTAATAAGAAAAATATGGCCT
  TGATGGTGTAGTGCATGGACATCTACCCAGATAAACAAATTGTTGACTGCTGAATTAATG
  TGATTTTGGTCTCTATTGATATTTCATAGGGCCCTGTCTTATTCAACTTTACTTTTAAAA
  TCAGTGATTTGGATGAAGGCATCAGAAACATCTGATTTGAGGATGGAGCAACCCAATATT
  GCTAATATAATAGATGACAGTGGCAACTGAAAACAGTCCCAGAATGACCCCATCGAGATG
  AAATTAAACAGATTAGAAATGTAACATACTCATTTAAATTAAATAAGTTGTATAAGTTTG
  GGCTGAAGAAACCTGGCCTTATATCATTTCATATAAAAAAGACGGGATTTTAGTTGTCCT
  CAGGTTCAATATGAACCAAGTAGTATTCATGTTGTTATTATATGATAACAGAAAAATTAG
  TGGTACTTCAGGTTTTATCAGTAGAAGTGTCACAGCCATAGTCAAAATACCCCTAACATA
  CTGCATTTCACTCTGGGTACCAAATTTAAGCAAGATATTGATGGCCACTAAGTGTGTATC
  CAGAAGAAGAGATCAGAATGATGAGTCCAGAACCCCTGTCACACAGGGAATAGGTGAAGG
  AACTAGGGATATTTAACTGGAGCAGAGAACATCTGGAGAGAGAGCAAGATTCCTGTCCTT
  GAGAAGTTGAAGCCGTCTCATGCAGAAGAGGGAATGAGCTTCCATTATGCTGCAGAGCTG
  GCAGCAATGGATGAAAGTTGCAGGAAGTTAGTTTTAGTTCTGTAGAAGGCAAGAGTAGGG
  TAGACCAAATCAAATGCTCACTGGGGTCAGGCTGATGGAGACAGTAACACTGGCGAGCAG
  GTGCCCCAGCTCGGACTATACTGCTGAGTTCTGGTCTGTTGCTGCCACTCTACAGGTCCA
  GGGTTGTTAGATCTTCTGGGTTTTTGTTTTGCTTTTTGAAATAAGGAAAATAAGCTTATC
  TTAAGTTTATTTTTCCTTGGATATACCTAGATTCCATACAGTGACAATACAAATACAAAG
  GAAGAACATCTGGATTTCATCCTTGACCTCATTTACAAAGCAAAAAGAGATGCTGGATTC
  AGATATAAATGTTTAATTTTGCAGTGTTTAAGTCAGCAAATCTTCTGTTTTTTAAAAATA
  AGCCACATATCTAGATTTTTCTGTGAAAGCTCTCAATTAAGTGTTGGGAACTAATTTCAA
  GATTTTAAAAAATGTTATGCAGGCTTAACGTGTCTGAGAGCCATAAATGACCTAACGTTT
  CCCGTTAGTCTCTGAACTAGGTGATCTCAGTTCTCTTTCAACTCCAGTACTCTGTAAGTT
  TCTGTGACCTGTAGTGTACCATTCTCAGGTGGTGATATGGTTTGGATGTTTTGTCCCCTC
  CAAATCTCATGTTCAAAGTGTGACATTCAGTGTTAGAGGTGGGCCTAGTAGGAGGTATTT
  GGGTCATGGGGGCGGATCCCTTATGTATGACTTTGTGCCATCCCCATAGTAATGAGTGAG
  TTCTCACTCTGGTTGTTTATGCAAGAGCTGGTTGTTTAAAAGAGCCTGGAACTTCCTCCT
  CTCTCGCTTGCTCTCTCTCACCATGTGACACACTGGCTCCCCTTCACCTTCTGCCATGAT
  TGTAAGCTTCGTGAGGCCCTCACCAGAAGCAGATGCCAGCGCCATGCTTCCTATACAGTT
  CTATGCAGAACAGTGAGCCAAATAAACCTCTTTTCTTTCTAAAAAAAAAAAAAAAAAAAA
  AAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_078470
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_078470.2, NP_510870.1
• RefSeq Size: 4081 bp
• RefSeq ORF: 1233 bp
• Locus ID: 1355
• Cytogenetics: 10q24.2
• Domains: COX15-CtaA
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Oxidative phosphorylation, Porphyrin and chlorophyll metabolism
• Gene Summary: 'Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates two transcript variants diverging in the 3' region. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.