PPCS (NM_001077447) Human Untagged Clone

CAT#: SC322766

PPCS (untagged)-Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPCS
• Synonyms: CMD2C
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322766 GGCGCCGGCCGCTGCGCTGCAGATGGCGGAAATGGATCCGGTAGCCGAGTTCCCCCAGCC
  TCCCGGTGCTGCGCGCTGGGCTGAGGTTATGGCTCGCTTCGCGGCCAGGCTGGGCGCGCA
  GGGCCGGCGGGTGGTGTTGGTTACGTCAGGCGGCACCAAGGTCCCACTGGAAGCGCGGCC
  GGTGCGCTTCCTGGACAACTTCAGCAGCGGGCGGCGCGGTGCAACCTCGGCCGAGGCCTT
  CCTAGCCGCCGGCTACGGGGTCCTGTTCTTGTATCGCGCTCGCTCTGCCTTCCCCTATGC
  CCACCGCTTCCCACCCCAGACTTGGCTGTCCGCTCTGCGGCCTTCGGGCCCAGCCCTTTC
  GGGCTTGCTGAGCCTGGAGGCCGAGGAGAATGCACTTCCGGGTTTTGCTGAGGCTCTGAG
  GAGCTACCAGGAGGCTGCGGCTGCAGGCACCTTCCTGGCAGTAGAGTTCACCACTTTGGC
  GGACTATTTGCATCTGTTGCAGGCTGCGGCCCAGGCACTCAATCCGCTAGGCCCTTCTGC
  GATGTTTTACCTGGCTGCGGCTGTGTCAGATTTCTATGTTCCTGTCTCTGAAATGCCTGA
  ACACAAGATCCAGTCATCTGGGGGCCCACTGCAGATAACAATGAAGATGGTGCCAAAACT
  GCTTTCTCCTTTGGTTAAAGATTGGGCTCCCAAAGCATTTATAATTTCCTTTAAGTTGGA
  GACTGACCCCGCCATTGTAATTAATCGAGCTCGGAAGGCTTTGGAAATTTATCAGCATCA
  AGTGGTGGTGGCTAATATCCTTGAGTCACGACAGTCCTTTGTGTTTATTGTAACCAAAGA
  CTCGGAAACCAAGTTATTGCTATCAGAGGAAGAAATAGAAAAAGGCGTAGAGATAGAAGA
  GAAGATAGTGGATAATCTTCAGTCTCGACACACAGCTTTTATAGGTGACAGAAACTGAAG
  TAAAAAGCCCTTATAGGATCAAAAATTGTTCAGGGCTCTTAGAGATGGTGAAAACTACAA
  AAAAAACCATGGCTTTCATATGGACAGAGAAAATGAAAGAAAGGGAAAAGGCAGTGGTGT
  GTAGGCAAATATGGTTTGGCATTTGTCTTTTAATGACACCTGATATGATGTCATTTTGAT
  TTTGAAATTGAACACTAGAACTGTTAATCACCTTTAAAAAGAAGAGCTTATTGGGAATTA
  TATATTCCTTAAAATATACATGGGGGCCTGAATGTCAGCCATCTTTATACTATAGAAAAA
  GGATTATGGATGCATGAATGGTCATGCTTTGGAGATCAAATATTGGTTGAATGCCTATGT
  ATGTCAGGCCCTGTGCTGAGCCATGAGGATTAAAAAGATGAATAAACATATCTTGTTTAG
  GAAATGGATGTATAAAAAAATCAAGTGCAATAAAGTGTGTGTCCAAAAGCTGACACAATG
  GAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for SC322766 unedited
  GCAGTATCTTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGT
  CCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGCGCCGGCCGCTGCGCTGCAGAT
  GGCGGAAATGGATCCGGTAGCCGAGTTCCCCCAGCCTCCCGGTGCTGCGCGCTGGGCTGA
  GGTTATGGCTCGCTTCGCGGCCAGGCTGGGCGCGCAGGGCCGGCGGGTGGTGTTGGTTAC
  GTCAGGCGGCACCAAGGTCCCACTGGAAGCGCGGCCGGTGCGCTTCCTGGACAACTTCAG
  CAGCGGGCGGCGCGGTGCAACCTCGGCCGAGGCCTTCCTAGCCGCCGGCTACGGGGTCCT
  GTTCTTGTATCGCGCTCGCTCTGCCTTCCCCTATGCCCACCGCTTCCCACCCCAGACTTG
  GCTGTCCGCTCTGCGGCCTTCGGGCCCAGCCCTTTCGGGCTTGCTGAGCCTGGAGGCCGA
  GGAGAATGCACTTCCGGGTTTTGCTGAGGCTCTGAGGAGCTACCAGGAGGCTGCGGCTGC
  AGGCACCTTCCTGGCAGTAGAGTTCACCACTTTGGCGGACTATTTGCATCTGTTGCAGGC
  TGCGGCCCAGCACTCAATCCGCTAGGCCCTTCTGCGATGTTTTACCTGGCCTGCGGCTGT
  GTCAGATTTCTATGTTCCTGTCTCTGAAATGCCTGAACACAGATCCAGTCATCTGGGGGC
  CCCACTGCAGATAACAATGAGATGGTGACCAAAACTGCTTTCCTCTTTGTTTAAAGATTG
  GCCTCCCAAGCATTAATATTTCCTTTAGTTTGAGACTGACCCCCGCCATTGTATTATCCG
  AGCCTCTACGCCTTGAATTATCAGCATCATGATGTGCTAATATCTGATTCACGACGTCTT
  TGGCTTATGACGAGACTCGGACACCAAGCTCTATCGTC
• Restriction Sites: Please inquire
• ACCN: NM_001077447
• ORF Size: 417 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001077447.1, NP_001070915.1
• RefSeq Size: 1044
• RefSeq ORF: 417
• Locus ID: 79717
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis
• Gene Summary: Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 5, 6, and 7 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.