SLC14A1 (NM_001128588) Human Untagged Clone

CAT#: SC323066

SLC14A1 (untagged)-Human solute carrier family 14 (urea transporter), member 1 (Kidd blood group) (SLC14A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC14A1
• Synonyms: HsT1341; HUT11; JK; RACH1; RACH2; UT-B1; UT1; UTE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001128588, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATGGACGGTCTTTGATTGGCGGCGCTGGTGACGCCCGTCATGGTCCTGTTTGGAAGGACCCTTTTG
  GAACTAAAGCTGGTGACGCAGCGCGCAGAGGCATCGCCCGGCTAAGCTTGGCCCTGGCAGATGGGTCGCA
  GGAACAGGAGCCAGAGGAAGAGATAGCCATGGAGGACAGCCCCACTATGGTTAGAGTGGACAGCCCCACT
  ATGGTTAGGGGTGAAAACCAGGTTTCGCCATGTCAAGGGAGAAGGTGCTTCCCCAAAGCTCTTGGCTATG
  TCACCGGTGACATGAAAGAACTTGCCAACCAGCTTAAAGACAAACCCGTGGTGCTCCAGTTCATTGACTG
  GATTCTCCGGGGCATATCCCAAGTGGTGTTCGTCAACAACCCCGTCAGTGGAATCCTGATTCTGGTAGGA
  CTTCTTGTTCAGAACCCCTGGTGGGCTCTCACTGGCTGGCTGGGAACAGTGGTCTCCACTCTGATGGCCC
  TCTTGCTCAGCCAGGACAGGTCATTAATAGCATCTGGGCTCTATGGCTACAATGCCACCCTGGTGGGAGT
  ACTCATGGCTGTCTTTTCGGACAAGGGAGACTATTTCTGGTGGCTGTTACTCCCTGTATGTGCTATGTCC
  ATGACTTGCCCAATTTTCTCAAGTGCATTGAATTCCATGCTCAGCAAATGGGACCTCCCCGTCTTCACCC
  TCCCTTTCAACATGGCGTTGTCAATGTACCTTTCAGCCACAGGACATTACAATCCATTCTTTCCAGCCAA
  ACTGGTCATACCTATAACTACAGCTCCAAATATCTCCTGGTCTGACCTCAGTGCCCTGGAGTTGTTGAAA
  TCTATACCAGTGGGAGTTGGTCAGATCTATGGCTGTGATAATCCATGGACAGGGGGCATTTTCCTGGGAG
  CCATCCTACTCTCCTCCCCACTCATGTGCCTGCATGCTGCCATAGGATCATTGCTGGGCATAGCAGCGGG
  ACTCAGTCTTTCAGCCCCATTTGAGGACATCTACTTTGGACTCTGGGGTTTCAACAGCTCTCTGGCCTGC
  ATTGCAATGGGAGGAATGTTCATGGCGCTCACCTGGCAAACCCACCTCCTGGCTCTTGGCTGTGCCCTGT
  TCACGGCCTATCTTGGAGTCGGCATGGCAAACTTTATGGCTGAGGTTGGATTGCCAGCTTGTACCTGGCC
  CTTCTGTTTGGCCACGCTATTGTTCCTCATCATGACCACAAAAAATTCCAACATCTACAAGATGCCCCTC
  AGTAAAGTTACTTATCCTGAAGAAAACCGCATCTTCTACCTGCAAGCCAAGAAAAGAATGGTGGAAAGCC
  CTTTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001128588
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128588.3, NP_001122060.3
• RefSeq Size: 4129 bp
• RefSeq ORF: 1338 bp
• Locus ID: 6563
• Cytogenetics: 18q12.3
• Protein Families: Transmembrane
• Gene Summary: 'The protein encoded by this gene is a membrane transporter that mediates urea transport in erythrocytes. This gene forms the basis for the Kidd blood group system. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.