CTSA (NM_001127695) Human Untagged Clone

CAT#: SC323092

CTSA (untagged)-Human cathepsin A (CTSA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTSA
• Synonyms: GLB2; GSL; NGBE; PPCA; PPGB
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001127695, the custom clone sequence may differ by one or more nucleotides
  ATGATCCGAGCCGCGCCGCCGCCGCTGTTCCTGCTGCTGCTGCTGCTGCTGCTGCTAGTG
  TCCTGGGCGTCCCGAGGCGAGGCAGCCCCCGACCAGGACGAGATCCAGCGCCTCCCCGGG
  CTGGCCAAGCAGCCGTCTTTCCGCCAGTACTCCGGCTACCTCAAAGGCTCCGGCTCCAAG
  CACCTCCACTACTGGTTTGTGGAGTCCCAGAAGGATCCCGAGAACAGCCCTGTGGTGCTT
  TGGCTCAATGGGGGTCCCGGCTGCAGCTCACTAGATGGGCTCCTCACAGAGCATGGCCCC
  TTCCTGGTCCAGCCAGATGGTGTCACCCTGGAGTACAACCCCTATTCTTGGAATCTGATT
  GCCAATGTGTTATACCTGGAGTCCCCAGCTGGGGTGGGCTTCTCCTACTCCGATGACAAG
  TTTTATGCAACTAATGACACTGAGGTCGCCCAGAGCAATTTTGAGGCCCTTCAAGATTTC
  TTCCGCCTCTTTCCGGAGTACAAGAACAACAAACTTTTCCTGACCGGGGAGAGCTATGCT
  GGCATCTACATCCCCACCCTGGCCGTGCTGGTCATGCAGGATCCCAGCATGAACCTTCAG
  GGGCTGGCTGTGGGCAATGGACTCTCCTCCTATGAGCAGAATGACAACTCCCTGGTCTAC
  TTTGCCTACTACCATGGCCTTCTGGGGAACAGGCTTTGGTCTTCTCTCCAGACCCACTGC
  TGCTCTCAAAACAAGTGTAACTTCTATGACAACAAAGACCTGGAATGCGTGACCAATCTT
  CAGGAAGTGGCCCGCATCGTGGGCAACTCTGGCCTCAACATCTACAATCTCTATGCCCCG
  TGTGCTGGAGGGGTGCCCAGCCATTTTAGGTATGAGAAGGACACTGTTGTGGTCCAGGAT
  TTGGGCAACATCTTCACTCGCCTGCCACTCAAGCGGATGTGGCATCAGGCACTGCTGCGC
  TCAGGGGATAAAGTGCGCATGGACCCCCCCTGCACCAACACAACAGCTGCTTCCACCTAC
  CTCAACAACCCGTACGTGCGGAAGGCCCTCAACATCCCGGAGCAGCTGCCACAATGGGAC
  ATGTGCAACTTTCTGGTAAACTTACAGTACCGCCGTCTCTACCGAAGCATGAACTCCCAG
  TATCTGAAGCTGCTTAGCTCACAGAAATACCAGATCCTATTATATAATGGAGATGTAGAC
  ATGGCCTGCAATTTCATGGGGGATGAGTGGTTTGTGGATTCCCTCAACCAGAAGATGGAG
  GTGCAGCGCCGGCCCTGGTTAGTGAAGTACGGGGACAGCGGGGAGCAGATTGCCGGCTTC
  GTGAAGGAGTTCTCCCACATCGCCTTTCTCACGATCAAGGGCGCCGGCCACATGGTTCCC
  ACCGACAAGCCCCTCGCTGCCTTCACCATGTTCTCCCGCTTCCTGAACAAGCAGCCATAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001127695
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127695.1, NP_001121167.1
• RefSeq Size: 2300 bp
• RefSeq ORF: 1443 bp
• Locus ID: 5476
• Cytogenetics: 20q13.12
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Renin-angiotensin system
• Gene Summary: 'This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform a.