NCF2 (NM_001127651) Human Untagged Clone

CAT#: SC323119

NCF2 (untagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2


  "NM_001127651" in other vectors (6)

Reconstitution Protocol

USD 760.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NCF2
Synonyms NCF-2; NOXA2; P67-PHOX; P67PHOX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001127651, the custom clone sequence may differ by one or more nucleotides


ATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGAAGGGGTGCTGGCAGCGGACAAGAAGGACTGGAAGG
GAGCCCTGGATGCCTTCAGTGCCGTCCAGGACCCCCACTCCCGGATTTGCTTCAACATTGGCTGCATGTA
CACTATCCTGAAGAACATGACTGAAGCAGAGAAGGCCTTTACCAGAAGCATTAACCGAGACAAGCACTTG
GCAGTGGCTTACTTCCAACGAGGGATGCTCTACTACCAGACAGAGAAATATGATTTGGCTATCAAAGACC
TTAAAGAAGCCTTGATTCAGCTTCGAGGGAACCAGCTGATAGACTATAAGATCCTGGGGCTCCAGTTCAA
GCTGTTTGCCTGTGAGGTGTTATATAACATTGCTTTCATGTATGCCAAGAAGGAGGAATGGAAAAAAGCT
GAAGAACAGTTAGCATTGGCCACGAGCATGAAGTCTGAGCCCAGACATTCCAAAATCGACAAGGCGATGG
AGTGTGTCTGGAAGCAGAAGCTATATGAGCCAGTGGTGATCCCTGTGGGCAAGCTGTTTCGACCAAATGA
GAGACAAGTGGCTCAGCTGGCCAAGAAGGATTACCTAGGCAAGGCGACGGTCGTGGCATCTGTGGTGGAT
CAAGACAGTTTCTCTGGGTTTGCCCCTCTGCAACCACAGGCAGCTGAGCCTCCACCCAGACCGAAAACCC
CAGAGATCTTCAGGGCTCTGGAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGTGCCTGAGACAAAAGA
AGAGCTCCAGGTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAAGGGCAATGATAACTGGGCCACGGTC
ATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAACTACCTTGAACCAGTTGAGCTGCGGATCCACCCTC
AGCAGCAGCCCCAGGAGGAAAGCTCTCCGCAGTCCGACATCCCAGCTCCTCCTAGTTCCAAAGCCCCTGG
AAGACCCCAGCTGTCACCAGGCCAGAAACAAAAAGAAGAGCCTAAGGAAGTGAAGCTCAGTGTTCCCATG
CCCTACACACTCAAGGTGCACTACAAGTACACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTACAGCC
AGGTCCGGGACATGGTGTCTAAGAAACTGGAGCTCCGGCTGGAACACACTAAGCTGAGCTATCGGCCTCG
GGACAGCAATGAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGATGCCTGGGGCCAGGTGAAAAACTAC
TGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGACCAAGGCTTTCCAGATGAACCCAAGGAAAGTGAAA
AAGCTGATGCTAATAACCAGACAACAGAACCTCAGCTTAAGAAAGGCAGCCAAGTGGAGGCACTCTTCAG
TTATGAGGCTACCCAACCAGAGGACCTGGAGTTTCAGGAAGGGGATATAATCCTGGTGTTATCAAAGGTG
AATGAAGAATGGCTGGAAGGGGAGTGCAAAGGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGAAGACT
GCGCAACTACAGATTTGGAAAGCACTCGGAGAGAAGTCTAG


Restriction Sites SgfI-MluI     
ACCN NM_001127651
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001127651.2, NP_001121123.1
RefSeq Size 2257 bp
RefSeq ORF 1581 bp
Locus ID 4688
Cytogenetics 1q25.3
Protein Families Druggable Genome
Protein Pathways Leukocyte transendothelial migration
Gene Summary 'This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]'
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.