NCF2 (NM_001127651) Human Untagged Clone

CAT#: SC323119

NCF2 (untagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCF2
• Synonyms: NCF-2; NOXA2; P67-PHOX; P67PHOX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001127651, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGAAGGGGTGCTGGCAGCGGACAAGAAGGACTGGAAGG
  GAGCCCTGGATGCCTTCAGTGCCGTCCAGGACCCCCACTCCCGGATTTGCTTCAACATTGGCTGCATGTA
  CACTATCCTGAAGAACATGACTGAAGCAGAGAAGGCCTTTACCAGAAGCATTAACCGAGACAAGCACTTG
  GCAGTGGCTTACTTCCAACGAGGGATGCTCTACTACCAGACAGAGAAATATGATTTGGCTATCAAAGACC
  TTAAAGAAGCCTTGATTCAGCTTCGAGGGAACCAGCTGATAGACTATAAGATCCTGGGGCTCCAGTTCAA
  GCTGTTTGCCTGTGAGGTGTTATATAACATTGCTTTCATGTATGCCAAGAAGGAGGAATGGAAAAAAGCT
  GAAGAACAGTTAGCATTGGCCACGAGCATGAAGTCTGAGCCCAGACATTCCAAAATCGACAAGGCGATGG
  AGTGTGTCTGGAAGCAGAAGCTATATGAGCCAGTGGTGATCCCTGTGGGCAAGCTGTTTCGACCAAATGA
  GAGACAAGTGGCTCAGCTGGCCAAGAAGGATTACCTAGGCAAGGCGACGGTCGTGGCATCTGTGGTGGAT
  CAAGACAGTTTCTCTGGGTTTGCCCCTCTGCAACCACAGGCAGCTGAGCCTCCACCCAGACCGAAAACCC
  CAGAGATCTTCAGGGCTCTGGAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGTGCCTGAGACAAAAGA
  AGAGCTCCAGGTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAAGGGCAATGATAACTGGGCCACGGTC
  ATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAACTACCTTGAACCAGTTGAGCTGCGGATCCACCCTC
  AGCAGCAGCCCCAGGAGGAAAGCTCTCCGCAGTCCGACATCCCAGCTCCTCCTAGTTCCAAAGCCCCTGG
  AAGACCCCAGCTGTCACCAGGCCAGAAACAAAAAGAAGAGCCTAAGGAAGTGAAGCTCAGTGTTCCCATG
  CCCTACACACTCAAGGTGCACTACAAGTACACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTACAGCC
  AGGTCCGGGACATGGTGTCTAAGAAACTGGAGCTCCGGCTGGAACACACTAAGCTGAGCTATCGGCCTCG
  GGACAGCAATGAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGATGCCTGGGGCCAGGTGAAAAACTAC
  TGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGACCAAGGCTTTCCAGATGAACCCAAGGAAAGTGAAA
  AAGCTGATGCTAATAACCAGACAACAGAACCTCAGCTTAAGAAAGGCAGCCAAGTGGAGGCACTCTTCAG
  TTATGAGGCTACCCAACCAGAGGACCTGGAGTTTCAGGAAGGGGATATAATCCTGGTGTTATCAAAGGTG
  AATGAAGAATGGCTGGAAGGGGAGTGCAAAGGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGAAGACT
  GCGCAACTACAGATTTGGAAAGCACTCGGAGAGAAGTCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001127651
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127651.2, NP_001121123.1
• RefSeq Size: 2257 bp
• RefSeq ORF: 1581 bp
• Locus ID: 4688
• Cytogenetics: 1q25.3
• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: 'This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform 1.