TRP2 (DCT) (NM_001129889) Human Untagged Clone

CAT#: SC323133

DCT (untagged)-Human dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) (DCT), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCT
• Synonyms: TRP-2; TYRP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001129889, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCCCCTTTGGTGGGGGTTTCTGCTCAGTTGCTTGGGCTGCAAAATCCTGCCAGGAGCCCAGGGTC
  AGTTCCCCCGAGTCTGCATGACGGTGGACAGCCTAGTGAACAAGGAGTGCTGCCCACGCCTGGGTGCAGA
  GTCGGCCAATGTCTGTGGCTCTCAGCAAGGCCGGGGGCAGTGCACAGAGGTGCGAGCCGACACAAGGCCC
  TGGAGTGGTCCCTACATCCTACGAAACCAGGATGACCGTGAGCTGTGGCCAAGAAAATTCTTCCACCGGA
  CCTGCAAGTGCACAGGAAACTTTGCCGGCTATAATTGTGGAGACTGCAAGTTTGGCTGGACCGGTCCCAA
  CTGCGAGCGGAAGAAACCACCAGTGATTCGGCAGAACATCCATTCCTTGAGTCCTCAGGAAAGAGAGCAG
  TTCTTGGGCGCCTTAGATCTCGCGAAGAAGAGAGTACACCCCGACTACGTGATCACCACACAACACTGGC
  TGGGCCTGCTTGGGCCCAATGGAACCCAGCCGCAGTTTGCCAACTGCAGTGTTTATGATTTTTTTGTGTG
  GCTCCATTATTATTCTGTTAGAGATACATTATTAGGACCAGGACGCCCCTACAGGGCCATAGATTTCTCA
  CATCAAGGACCTGCATTTGTTACCTGGCACCGGTACCATTTGTTGTGTCTGGAAAGAGATCTCCAGCGAC
  TCATTGGCAATGAGTCTTTTGCTTTGCCCTACTGGAACTTTGCCACTGGGAGGAACGAGTGTGATGTGTG
  TACAGACCAGCTGTTTGGGGCAGCGAGACCAGACGATCCGACTCTGATTAGTCGGAACTCAAGATTCTCC
  AGCTGGGAAACTGTCTGTGATAGCTTGGATGACTACAACCACCTGGTCACCTTGTGCAATGGAACCTATG
  AAGGTTTGCTGAGAAGAAATCAAATGGGAAGAAACAGCATGAAATTGCCAACCTTAAAAGACATACGAGA
  TTGCCTGTCTCTCCAGAAGTTTGACAATCCTCCCTTCTTCCAGAACTCTACCTTCAGTTTCAGGAATGCT
  TTGGAAGGGTTTGATAAAGCAGATGGGACTCTGGATTCTCAAGTGATGAGCCTTCATAATTTGGTTCATT
  CCTTCCTGAACGGGACAAACGCTTTGCCACATTCAGCCGCCAATGATCCCATTTTTGTGGTGATTTCTAA
  TCGTTTGCTTTACAATGCTACAACAAACATCCTTGAACATGTAAGAAAAGAGAAAGCGACCAAGGAACTC
  CCTTCCCTGCATGTGCTGGTTCTTCATTCCTTTACTGATGCCATCTTTGATGAGTGGATGAAAAGATTTA
  ATCCTCCTGCAGATGCCTGGCCTCAGGAGCTGGCCCCTATTGGTCACAATCGGATGTACAACATGGTTCC
  TTTCTTCCCTCCAGTGACTAATGAAGAACTCTTTTTAACCTCAGACCAACTTGGCTACAGCTATGCCATC
  GATCTGCCAGTTTCAGTTGAAGAAACTCCAGGTTGGCCCACAACTCTCTTAGTAGTCATGGGAACACTGG
  TGGCTTTGGTTGGTCTTTTTGTGCTGTTGGCTTTTCTTCAATATAGAAGACTTCGAAAAGGATATACACC
  CCTAATGGAGACACATTTAAGCAGCAAGAGATACACAGAAGAAGCCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001129889
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129889.2, NP_001123361.1
• RefSeq Size: 4680 bp
• RefSeq ORF: 1659 bp
• Locus ID: 1638
• Cytogenetics: 13q32.1
• Protein Families: Transmembrane
• Protein Pathways: Melanogenesis, Metabolic pathways, Tyrosine metabolism
• Gene Summary: ''
  Transcript Variant: This variant (2) includes two alternate in-frame exons and is predicted to encode a slightly longer protein isoform (2) compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.