ELF4 (NM_001127197) Human Untagged Clone

CAT#: SC323170

ELF4 (untagged)-Human E74-like factor 4 (ets domain transcription factor) (ELF4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELF4
• Synonyms: ELFR; MEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001127197, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTATTACCCTACAGCCCAGTGACCTGATCTTTGAGTTCGCAAGCAACGGGATGGATGATGATATCC
  ACCAGCTGGAAGACCCCTCTGTGTTCCCAGCTGTGATCGTGGAGCAGGTACCCTACCCTGATTTACTGCA
  TCTGTACTCGGGACTGGAGTTGGACGACGTTCACAATGGCATCATAACAGACGGGACCTTGTGCATGACG
  CAGGATCAGATCCTGGAAGGCAGTTTTTTGCTGACAGATGACAATGAGGCCACCTCGCACACCATGTCAA
  CCGCGGAAGTCTTACTCAATATGGAGTCTCCCAGCGATATCCTGGATGAGAAGCAGATCTTCAGTACCTC
  CGAAATGCTTCCAGACTCGGACCCTGCACCAGCTGTCACTCTGCCCAACTACCTGTTTCCTGCCTCTGAG
  CCCGATGCCCTGAACAGGGCGGGTGACACTAGTGACCAGGAGGGGCATTCTCTGGAGGAGAAGGCCTCCA
  GAGAGGAAAGTGCCAAGAAGACTGGGAAATCAAAGAAGAGAATCCGGAAGACCAAGGGCAACCGAAGTAC
  CTCACCTGTCACTGACCCCAGCATCCCCATTAGGAAGAAATCAAAGGATGGCAAAGGCAGCACCATCTAT
  CTGTGGGAGTTCCTCCTGGCTCTTCTGCAAGACAGAAACACCTGTCCCAAGTACATCAAGTGGACCCAGC
  GAGAGAAAGGCATCTTCAAACTGGTGGACTCCAAAGCTGTGTCCAAGCTGTGGGGGAAGCAGAAAAACAA
  GCCTGACATGAACTATGAGACAATGGGGCGGGCACTAAGATACTACTACCAAAGAGGCATACTGGCCAAA
  GTGGAAGGGCAGAGGCTGGTGTACCAGTTTAAGGAGATGCCCAAGGACCTGGTGGTCATTGAAGATGAGG
  ATGAGAGCAGCGAAGCCACAGCAGCCCCACCTCAGGCCTCCACGGCCTCTGTGGCCTCTGCCAGTACCAC
  CCGGCGAACCAGCTCCAGGGTCTCATCCAGATCTGCCCCCCAGGGCAAGGGCAGCTCTTCTTGGGAGAAG
  CCAAAAATTCAGCATGTCGGTCTCCAGCCATCTGCGAGTCTGGAATTGGGACCGTCGCTAGACGAGGAGA
  TCCCCACTACCTCCACCATGCTCGTCTCTCCAGCAGAGGGCCAGGTCAAGCTCACCAAAGCTGTGAGTGC
  ATCTTCAGTGCCCAGCAACATCCACCTAGGAGTGGCCCCCGTGGGGTCGGGCTCGGCCCTGACCCTGCAG
  ACGATCCCACTGACCACGGTGCTGACCAATGGGCCTCCTGCCAGTACTACTGCTCCCACTCAGCTCGTTC
  TCCAGAGTGTTCCAGCGGCCTCTACTTTCAAGGACACCTTCACTTTGCAGGCCTCTTTCCCCCTGAACGC
  CAGTTTCCAAGACAGCCAGGTGGCAGCCCCAGGGGCTCCACTGATTCTCAGTGGCCTCCCCCAACTTCTG
  GCTGGGGCCAACCGTCCGACCAACCCGGCGCCACCCACGGTCACAGGGGCTGGACCAGCAGGGCCCAGCT
  CTCAGCCCCCTGGGACTGTCATTGCTGCCTTCATCAGGACTTCTGGCACTACAGCAGCCCCTAGGGTCAA
  GGAGGGGCCACTGAGGTCCTCCTCCTATGTTCAGGGTATGGTGACGGGGGCCCCCATGGAGGGGCTGCTG
  GTTCCTGAAGAGACCCTGAGGGAGCTCCTGAGAGATCAGGCTCATCTTCAGCCACTTCCAACCCAGGTGG
  TTTCCAGGGGTTCCCACAATCCGAGCCTTCTGGGCAACCAGACTTTGTCTCCTCCCAGCCGCCCCACTGT
  TGGGCTGACCCCAGTGGCTGAACTTGAGCTCTCCTCAGGCTCAGGGTCCCTGCTGATGGCTGAGCCTAGT
  GTGACCACATCTGGGAGCCTTCTGACAAGATCCCCCACCCCAGCCCCTTTCTCCCCATTCAACCCTACTT
  CCCTCATTAAGATGGAGCCCCATGACATATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001127197
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127197.1, NP_001120669.1
• RefSeq Size: 4096 bp
• RefSeq ORF: 1992 bp
• Locus ID: 2000
• Cytogenetics: Xq26.1
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a transcriptional activator that binds and activates the promoters of the CSF2, IL3, IL8, and PRF1 genes. The encoded protein is involved in natural killer cell development and function, innate immunity, and induction of cell cycle arrest in naive CD8+ cells. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.