PMS1 (NM_001128143) Human Untagged Clone

CAT#: SC323228

PMS1 (untagged)-Human PMS1 postmeiotic segregation increased 1 (S. cerevisiae) (PMS1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMS1
• Synonyms: HNPCC3; hPMS1; MLH2; PMSL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001128143, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAACAATTGCCTGCGGCAACAGTTCGACTCCTTTCAAGTTCTCAGATCATCACTTCGGTGGTCAGTG
  TTGTAAAAGAGCTTATTGAAAACTCCTTGGATGCTGGTGCCACAAGCGTAGATGTTAAACTGGAGAACTA
  TGGATTTGATAAAATTGAGGTGCGAGATAACGGGGAGGGTATCAAGGCTGTTGATGCACCTGTAATGGCA
  ATGAAGTACTACACCTCAAAAATAAATAGTCATGAAGATCTTGAAAATTTGACAACTTACGGTTTTCGTG
  GAGAAGCCTTGGGGTCAATTTGTTGTATAGCTGAGGTTTTAATTACAACAAGAACGGCTGCTGATAATTT
  TAGCACCCAGTATGTTTTAGATGGCAGTGGCCACATACTTTCTCAGAAACCTTCACATCTTGGTCAAGGT
  ACAACTGTAACTGCTTTAAGATTATTTAAGAATCTACCTGTAAGAAAGCAGTTTTACTCAACTGCAAAAA
  AATGTAAAGATGAAATAAAAAAGATCCAAGATCTCCTCATGAGCTTTGGTATCCTTAAACCTGACTTAAG
  GATTGTCTTTGTACATAACAAGATTTATCTCAGTGGATTTCTTCCAAAGTGTGATGCAGACCACTCTTTC
  ACTAGTCTTTCAACACCAGAAAGAAGTTTCATCTTCATAAACAGTCGACCAGTACATCAAAAAGATATCT
  TAAAGTTAATCCGACATCATTACAATCTGAAATGCCTAAAGGAATCTACTCGTTTGTATCCTGTTTTCTT
  TCTGAAAATCGATGTTCCTACAGCTGATGTTGATGTAAATTTAACACCAGATAAAAGCCAAGTATTATTA
  CAAAATAAGGAATCTGTTTTAATTGCTCTTGAAAATCTGATGACGACTTGTTATGGACCATTACCTAGTA
  CAAATTCTTATGAAAATAATAAAACAGATGTTTCCGCAGCTGACATCGTTCTTAGTAAAACAGCAGAAAC
  AGATGTGCTTTTTAATAAAGTGGAATCATCTGGAAAGAATTATTCAAATGTTGATACTTCAGTCATTCCA
  TTCCAAAATGATATGCATAATGATGAATCTGGAAAAAACACTGATGATTGTTTAAATCACCAGATAAGTA
  TTGGTGACTTTGGTTATGGTCATTGTAGTAGTGAAATTTCTAACATTGATAAAAACACTAAGAATGCATT
  TCAGGACATTTCAATGAGTAATGTATCATGGGAGAACTCTCAGACGGAATATAGTAAAACTTGTTTTATA
  AGTTCCGTTAAGCACACCCAGTCAGAAAATGGCAATAAAGACCATATAGATGAGAGTGGGGAAAATGAGG
  AAGAAGCAGGTCTTGAAAACTCTTCGGAAATTTCTGCAGATGAGTGGAGCAGGGGAAATATACTTAAAAA
  TTCAGTGGGAGAGAATATTGAACCTGTGAAAATTTTAGTGCCTGAAAAAAGTTTACCATGTAAAGTAAGT
  AATAATAATTATCCAATCCCTGAACAAATGAATCTTAATGAAGATTCATGTAACAAAAAATCAAATGTAA
  TAGATAATAAATCTGGAAAAGTTACAGCTTATGATTTACTTAGCAATCGAGTAATCAAGAAACCCATGTC
  AGCAAGTGCTCTTTTTGTTCAAGATCATCGTCCTCAGTTTCTCATAGAAAATCCTAAGACTAGTTTAGAG
  GATGCAACACTACAAATTGAAGAACTGTGGAAGACATTGAGTGAAGAGGAAAAACTGAAATATGAAGAGA
  AGGCTACTAAAGACTTGGAACGATACAATAGTCAAATGAAGAGAGCCATTGAACAGGAGTCACAAATGTC
  ACTAAAAGATGGCAGAAAAAAGATAAAACCCACCAGCGCATGGAATTTGGCCCAGAAGCACAAGTTAAAA
  ACCTCATTATCTAATCAACCAAAACTTGATGAACTCCTTCAGTCCCAAATTGAAAAAAGAAGGAGTCAAA
  ATATTAAAATGGTACAGATCCCCTTTTCTATGAAAAACTTAAAAATAAATTTTAAGAAACAAAACAAAGT
  TGACTTAGAAGAGAAGGATGAACCTTGCTTGATCCACAATCTCAGGTTTCCTGATGCATGGCTAATGACA
  TCCAAAACAGAGGTAATGTTATTAAATCCATATAGAGTAGAAGAAGCCCTGCTATTTAAAAGACTTCTTG
  AGAATCATAAACTTCCTGCAGAGCCACTGGAAAAGCCAATTATGTTAACAGAGAGTCTTTTTAATGGATC
  TCATTATTTAGACGTTTTATATAAAATGACAGCAGATGACCAAAGATACAGTGGATCAACTTACCTGTCT
  GATCCTCGTCTTACAGCGAATGGTTTCAAGATAAAATTGATACCAGGAGTTTCAATTACTGAAAATTACT
  TGGAAATAGAAGGAATGGCTAATTGTCTCCCATTCTATGGAGTAGCAGATTTAAAAGAAATTCTTAATGC
  TATATTAAACAGAAATGCAAAGGAAGTTTATGAATGTAGACCTCGCAAAGTGATAAGTTATTTAGAGGGA
  GAAGCAGTGCGTCTATCCAGACAATTACCCATGTACTTATCAAAAGAGGACATCCAAGACATTATCTACA
  GAATGAAGCACCAGTTTGGAAATGAAATTAAAGAGTGTGTTCATGGTCGCCCATTTTTTCATCATTTAAC
  CTATCTTCCAGAAACTACATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001128143
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128143.1, NP_001121615.1
• RefSeq Size: 3421 bp
• RefSeq ORF: 2682 bp
• Locus ID: 5378
• Cytogenetics: 2q32.2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a protein belonging to the DNA mismatch repair mutL/hexB family. This protein is thought to be involved in the repair of DNA mismatches, and it can form heterodimers with MLH1, a known DNA mismatch repair protein. Mutations in this gene cause hereditary nonpolyposis colorectal cancer type 3 (HNPCC3) either alone or in combination with mutations in other genes involved in the HNPCC phenotype, which is also known as Lynch syndrome. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) is also known as PMS1 nirs variant 7.