GEN1 (NM_001130009) Human Untagged Clone

CAT#: SC323232

GEN1 (untagged)-Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GEN1
• Synonyms: Gen
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001130009, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGTGAATGACTTGTGGCAAATTTTGGAGCCTGTTAAGCAACACATCCCCTTGCGTAATCTTGGTG
  GGAAAACCATTGCAGTTGATCTGAGTCTCTGGGTGTGTGAGGCACAGACAGTCAAAAAAATGATGGGCAG
  CGTCATGAAGCCCCACCTCAGGAACTTATTTTTTCGTATCTCATATTTAACACAAATGGATGTAAAACTG
  GTATTTGTTATGGAAGGGGAACCACCAAAGCTGAAAGCTGATGTCATAAGCAAGAGGAATCAGTCTCGGT
  ATGGGTCTTCTGGAAAATCGTGGTCTCAGAAAACAGGGAGATCACATTTTAAATCAGTCTTAAGAGAGTG
  CCTCCATATGCTCGAATGCTTAGGAATCCCCTGGGTTCAGGCTGCTGGGGAAGCTGAAGCCATGTGTGCT
  TATCTCAATGCTGGTGGTCATGTCGATGGCTGCCTCACCAATGATGGAGATACTTTCCTTTATGGGGCCC
  AGACTGTTTACAGGAATTTCACTATGAATACAAAGGACCCACATGTTGACTGTTACACAATGTCATCTAT
  CAAGAGTAAACTAGGTTTGGATAGAGATGCTCTGGTTGGATTAGCAATACTTCTTGGCTGTGATTATCTC
  CCAAAGGGAGTCCCTGGAGTTGGAAAAGAGCAAGCATTAAAACTTATACAGATTTTGAAAGGGCAAAGTT
  TACTTCAGAGGTTTAATCGGTGGAATGAAACATCTTGTAACTCTAGTCCACAACTGCTAGTCACTAAAAA
  ACTGGCTCATTGTTCCGTATGTTCCCATCCAGGTTCACCTAAGGATCATGAACGTAATGGATGCAGATTA
  TGTAAAAGTGATAAATATTGTGAGCCACATGACTATGAATACTGCTGTCCTTGTGAGTGGCACCGTACAG
  AACATGATAGGCAACTCAATGAAGTAGAGAACAATATTAAGAAGAAAGCTTGCTGTTGTGAGGGATTCCC
  ATTCCATGAGGTTATTCAAGAATTCCTTTTAAACAAGGATAAATTGGTGAAGGTTATCAGGTACCAAAGA
  CCTGATTTGTTATTGTTTCAGAGATTTACTCTTGAAAAAATGGAGTGGCCCAATCACTATGCATGTGAGA
  AATTGCTGGTACTTTTGACCCATTATGACATGATAGAAAGAAAGCTTGGTAGCAGAAACTCTAATCAACT
  ACAGCCAATTCGAATTGTTAAGACTCGAATCAGAAATGGAGTTCATTGTTTTGAAATAGAATGGGAAAAG
  CCTGAACATTATGCTATGGAAGATAAACAACATGGAGAATTTGCTTTATTAACAATTGAGGAAGAATCAT
  TGTTTGAAGCAGCATATCCTGAGATCGTTGCTGTTTACCAAAAACAAAAGTTAGAAATTAAAGGGAAGAA
  ACAAAAACGTATTAAGCCTAAAGAAAACAATTTGCCAGAACCAGATGAAGTAATGAGCTTTCAGTCACAC
  ATGACTTTAAAACCCACATGTGAAATCTTTCATAAGCAGAATTCCAAGTTAAATTCGGGGATTTCCCCTG
  ATCCTACATTACCACAGGAATCTATTTCTGCCTCATTGAATAGCTTGCTTTTACCTAAAAATACTCCATG
  TTTGAATGCACAAGAACAGTTCATGTCTTCTCTAAGACCTTTGGCTATACAGCAAATTAAAGCTGTCAGT
  AAGTCTCTAATTTCAGAATCTAGTCAACCCAATACCTCATCTCATAATATATCCGTGATTGCTGATCTAC
  ACTTGAGCACTATTGACTGGGAAGGTACTTCTTTTAGTAATTCTCCAGCTATTCAAAGGAATACTTTTTC
  TCATGATTTAAAATCAGAAGTTGAATCAGAGCTATCAGCCATCCCTGATGGCTTTGAAAATATCCCAGAA
  CAACTGTCCTGTGAATCAGAAAGGTACACTGCAAACATAAAGAAAGTGTTGGATGAGGATTCTGATGGGA
  TTAGTCCTGAAGAGCATCTACTTTCTGGCATTACTGATTTATGTCTTCAGGATTTGCCTTTAAAGGAACG
  AATATTTACAAAATTATCATATCCTCAGGATAATCTACAACCAGATGTCAACCTGAAAACTTTGTCCATA
  CTTAGTGTAAAAGAATCTTGTATTGCTAACAGTGGTTCTGATTGTACATCACATCTTTCAAAGGATCTTC
  CAGGAATTCCCTTGCAAAATGAATCCAGAGACTCTAAAATTCTAAAAGGAGACCAGCTGCTTCAAGAAGA
  CTATAAAGTCAATACTTCTGTCCCTTATTCTGTCAGTAACACAGTGGTAAAGACCTGCAATGTTAGACCA
  CCAAATACTGCTTTAGATCATAGTAGAAAAGTTGATATGCAAACCACTCGGAAAATTTTAATGAAGAAGA
  GTGTTTGCCTTGACAGACATTCCTCTGATGAACAAAGTGCCCCAGTGTTTGGGAAAGCTAAGTACACAAC
  TCAAAGAATGAAGCACAGTTCTCAAAAGCATAATTCATCCCATTTCAAAGAAAGTGGCCATAACAAGTTG
  AGTAGCCCTAAGATACATATTAAAGAAACTGAACAGTGTGTCAGATCTTATGAAACAGCTGAAAATGAAG
  AAAGCTGTTTCCCAGATTCAACAAAAAGTTCTCTGAGTTCTCTACAATGTCATAAGAAAGAAAACAACTC
  TGGTACTTGTTTGGATAGCCCTCTTCCTTTACGCCAGAGATTAAAACTAAGATTCCAAAGCACTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130009
• ORF Size: 2727 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001130009.2, NP_001123481.2
• RefSeq Size: 6931
• RefSeq ORF: 2727
• Locus ID: 348654
• Gene Summary: This gene encodes a member of the Rad2/xeroderma pigmentosum group G nuclease family, whose members are characterized by N-terminal and internal xeroderma pigmentosum group G nuclease domains followed by helix-hairpin-helix domains and disordered C-terminal domains. The protein encoded by this gene is involved in resolution of Holliday junctions, which are intermediate four-way structures that covalently link DNA during homologous recombination and double-strand break repair. The protein resolves Holliday junctions by creating dual incisions across the junction to produce nicked duplex products that can be ligated. In addition, this protein has been found to localize to centrosomes where it has been implicated in regulation of centrosome integrity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence data consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments.